Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Kaempferol Inhibits Enterovirus Proliferation through MAPK Signal Regulation

Kaempferol의 MAPK 신호 조절을 통한 심근염 유발 엔테로바이러스 증식 억제

  • Jang, Jin-Hwa (Department of Biomedical Science, Jungwon University) ;
  • Jeong, Hae-In (Department of Chemistry and Nano Science, Ewha Womans University) ;
  • Lim, Byung-Kwan (Department of Biomedical Science, Jungwon University) ;
  • Nam, Sang-Jip (Department of Chemistry and Nano Science, Ewha Womans University)
  • 장진화 (중원대학교 의생명과학과) ;
  • 정해인 (이화여자대학교 화학.나노과학과) ;
  • 임병관 (중원대학교 의생명과학과) ;
  • 남상집 (이화여자대학교 화학.나노과학과)
  • Received : 2017.07.24
  • Accepted : 2017.09.14
  • Published : 2017.09.30
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Abstract

We investigated the efficacy of single compound of plant extract in coxsackievirus B3 (CVB3) infection. CVB3 is a main cause of Hand-foot-mouth diseases (HFMD) and viral myocarditis in children and adult. Several single compounds of plant extract were purified by HPLC and tested as antiviral drug candidate. Among them, kaempferol was selected to effective anti-enterovirus compound by HeLa cells survival assay. CVB3 infected HeLa cells were treated with kaempferol ($100{\mu}g/ml-100ng/ml$) and their antiviral effect was confirmed. After 16 hours of treatment, HeLa cells were lysed and proteins were extracted for western blot analysis. CVB3 viral capsid protein VP1 production and transcription factor eIF4G-1 cleavage was significantly decreased in $100{\mu}g/ml$ kaempferol treatment. Virus replication was observed by virus RNA amplification. Kaempferol strongly reduced virus positive and negative strand RNA amplification. Moreover, MAPK signal induced by CVB3 infection, pERK and pmTOR, kaempferol treatment significantly inhibited the activity. Plant extract single compound, kaempferol, is a strong candidate to be developed non-toxic anti-enterovirus treatment agent.

Keywords

References

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