한국응용과학기술학회지 (Journal of the Korean Applied Science and Technology)

  • 제34권2호
  • /
  • Pages.225-235
  • /
  • 2017
  • /
  • 1225-9098(pISSN)
  • /
  • 2288-1069(eISSN)
한국응용과학기술학회 (The Korean Society of Applied Science and Technology)
권호 표지
DOI QR코드

DOI QR Code

레조시놀다이펜틸에터를 함유한 O/W 나노에멀젼의 경피흡수 및 미백 효능 효과 연구

Studies on skin whitening efficacy and skin permeation using O/W Nanoemulsion system with Resorcinol Dipentyl Ether

  • 차영권 ((주)코스메카코리아 기술연구원) ;
  • 조현대 ((주)코스메카코리아 기술연구원) ;
  • 조완구 (전주대학교 바이오기능성식품학과) ;
  • 변상요 (아주대학교 응용생명공학과)
  • 투고 : 2017.05.08
  • 심사 : 2017.06.20
  • 발행 : 2017.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

본 연구에서는 피부미백제로 개발된 레조시놀다이펜틸에터[1,3-di(pentyloxyl)benzene]를 함유하여 조성 상전이 방법(Phase Inversion Composition, PIC)방법으로 제조된 RS-Nanoemulsion의 경피 흡수 및 피부 미백 효능효과에 대해서 연구하였다. 지표성분인 1,3-di(pentyloxyl)benzene를 mineral oil에 용해한 대조시료와 일반 O/W유화법으로 제조된 RS-emulsion시료를 비교 대상으로 인체 피부에 대한 경피 흡수시험을 수행하였다. 시험결과, RS-Nanoemulsion 시료는 지표성분인 1,3-di(pentyloxyl) benzene를 mineral oil에 용해한 대조시료와 RS-emulsion 시료에 비해 통계적으로 유의하게 높은 지표성분 경피 투과율을 나타내었다. RS-Nanoemulsion을 O/W크림에 함유하여 만든 크림 제형의 피부임상시험을 통하여 그 유효성을 평가하고자 하였으며, 상기 시험결과 시험제품과 대조제품 모두 8주간의 시험기간에 특별한 이상반응이 관찰되지 않아 안전한 제품으로 확인되었고, 사용 8주 후 평가에서 대조제품 사용에 비해 시험제품이 통계적으로 유의한 수준으로 피부미백에 도움을 주는 효과가 있는 것을 확인하였다.

In this study, we investigated the effect on the increase of In vitro skin permeation experiments and In-vivo skin whitening efficacy using a O/W nanoemulsion produced via PIC(Phase Inversion Composition) with 1,3-di(pentyloxyl)benzene. skin permeation experiments of RS-nanoemulsion formulated with selected condition was evaluated compared to mineral oil containing 1,3-di(pentyloxyl)benzene and normal O/W type RS-emulsion. Compared to mineral oil with 1,3-di(pentyloxyl)benzene and RS-emulsion. RS-Nanoemulsion has a statistically significant high percutaneous absorption in terms of index substance, which is 1,3-di(pentyloxyl)benzene. In vivo test were prepared in the system of O/W cream containing RS-nanoemulsion. There was no adverse reactions in both samples. After 8 weeks, the subjects was evaluated by a dermatologist's scoring and Chromameter. In conclusion, the testing product showed statistically improvement (p<0.05) compared to the controlled product and proved its whitening efficacy.

키워드

참고문헌

  1. S. Briganti, E. Camera, and M. Picardo, "Chemical and instrumental approaches to treat hyperpigmentation", Pigment. Cell. Res., Vol.16, No.2 pp. 101-110, (2003). https://doi.org/10.1034/j.1600-0749.2003.00029.x
  2. W. G. Cho, C. T. Kim, S. H. Choi, and Y. K. Cha, Report of global cosmetic projects of Ministry of Health & Welfare, Republic of Korea (Project No.HN12C0056), (2014).
  3. A. Forgiarini, J. Esquena, C. Gonzalez, and C. Solans, "Formation of nano- emulsions by low-energy emulsification methods at constant temperature", Langmuir, Vol.17, No.7 pp. 2076-2083. (2001). https://doi.org/10.1021/la001362n
  4. P. Fernandez, V. Andre, J. Rieger, and A. Kuhnle, "Nano-emulsion formation by emulsion phase inversion", Colloids Surf., Vol.251, pp. 53-58, (2004). https://doi.org/10.1016/j.colsurfa.2004.09.029
  5. W. Liu, D. Sun, C. Li, Q. Liu, and J. Xu, "Formation and stability of paraffin oil-in-water nano-emulsions prepared by the emulsion inversion point method", J. Colloid Interface Sci., Vol.303, No.2 pp. 557-563, (2006). https://doi.org/10.1016/j.jcis.2006.07.055
  6. O. Sonneville-Aubrun, D. Babayan, D. Bordeaux, P. Lindner, G. Rata, and B. Cabane, "Phase transition pathways for the production of 100 nm oil-in-water emulsions", Phys. Chem. Chem. Phys., Vol.11, No.1 pp. 101-110. 2009. https://doi.org/10.1039/B813502A
  7. Elsayed MA, Abdallah Y, Naggar F, Khalafallah M, "Deformable liposomes and ethosomes: Mechanism of enhanced skin delivery", Int. J. Pharm., Vol.332, No.1-2 pp. 60-66, (2006).
  8. Honeywell-Nguyen PL, Bouwstra JA, "Vesicles as a tool for transdermal and dermal delivery", Drug Discovery Today:Technologies, Vol.2, No.1 pp. 67-74, (2005). https://doi.org/10.1016/j.ddtec.2005.05.003
  9. J. H. Kim, H. J. Yang, B. R. Won, Y. J. Ahn, M. K. Kang, and S. N. Park, "Preparation of Vitamin E Acetate Nano-emulsion and In Vitro Research Regarding Vitamin E Acetate Transdermal Delivery System which Use Franz Diffusion Cell", J. Soc. Cosmet. Scientists Korea, Vol.35, No.2 pp. 91-101, (2009).
  10. OECD (2004). Test Guideline 428: Skin absorption: in vitro Method. OECD, Paris.
  11. OECD (2004). Test Guideline 427: Skin absorption: in vivo Method. OECD, Paris.
  12. OECD (2004). Guidance Document for the Conduct of Skin Absorption Studies. OECD, Paris.
  13. Diembeck W, Beck H, Benech-Kieffer F, Courtellemont P, Dupuis J, Lovell W, Paye M, Spengler J, Steiling W, "Test Guidelines for In Vitro Assessment of Dermal Absorption and Percutaneous Penetration of Cosmetic Ingredients", Food Chem Toxicol, Vol.37, No.1-2 pp. 191-205, (1999). https://doi.org/10.1016/S0278-6915(98)00114-8
  14. Jewell C, Heylings JR, Clowes, HM. And Williams, FM, "Percutaneous absorption and metabolism of dinitrochlorobenzene in vitro", Arch Toxicol, Nol.74, No.7 pp. 356-365, (2000). https://doi.org/10.1007/s002040000123
  15. KFDA (2010). Guideline for in vitro Skin Absorption method. KFDA, Seoul.