Parasites, Hosts and Diseases

  • 제55권4호
  • /
  • Pages.451-455
  • /
  • 2017
  • /
  • 2982-5164(pISSN)
  • /
  • 1738-0006(eISSN)
대한기생충학·열대의학회 (The Korea Society for Parasitology and Tropical Medicine)
권호 표지
DOI QR코드

DOI QR Code

Characterization of Echinostoma cinetorchis endoribonuclease, RNase H

  • Lim, Sung-Bin (Department of Tropical Medicine and Parasitology, Inha University School of Medicine) ;
  • Cha, Seok Ho (Department of Tropical Medicine and Parasitology, Inha University School of Medicine) ;
  • Jegal, Seung (Department of Tropical Medicine and Parasitology, Inha University School of Medicine) ;
  • Jun, Hojong (Department of Tropical Medicine and Parasitology, Inha University School of Medicine) ;
  • Park, Seo Hye (Department of Tropical Medicine and Parasitology, Inha University School of Medicine) ;
  • Jeon, Bo-Young (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, School of Public Health) ;
  • Pak, Jhang Ho (Department of Convergence Medicine, University of Ulsan, College of Medicine, Asan Institute for Life Sciences, Asan Medical Center) ;
  • Bakh, Young Yil (Department of Biotechnology, Konkuk University) ;
  • Kim, Tong-Soo (Department of Tropical Medicine and Parasitology, Inha University School of Medicine) ;
  • Lee, Hyeong-Woo (Institute of Research and Development, Scorpiogen Co.)
  • 투고 : 2016.09.16
  • 심사 : 2017.08.02
  • 발행 : 2017.08.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Echinostoma cinetorchis is an oriental intestinal fluke causing significant pathological damage to the small intestine. The aim of this study was to determine a full-length cDNA sequence of E. cinetorchis endoribonuclease (RNase H; EcRNH) and to elucidate its molecular biological characters. EcRNH consisted of 308 amino acids and showed low similarity to endoribonucleases of other parasites (<40%). EcRNH had an active site centered on a putative DDEED motif instead of DEDD conserved in other species. A recombinant EcRNH produced as a soluble form in Escherichia coli showed enzymatic activity to cleave the 3'-O-P bond of RNA in a DNA-RNA duplex, producing 3'-hydroxyl and 5'-phosphate. These findings may contribute to develop antisense oligonucleotides which could damage echinostomes and other flukes.

키워드

참고문헌

  1. Yamashita J. Echinostome. Progr Med Parasitol Japan 1964; 1:289-313.
  2. Ando R, Ozaki Y. On four new species of trematodes of the family Echinostomatidae. Dobutsugaku Zasshi 1923; 5: 108-119 (in Japanese).
  3. Takahashi S, Ishii T, Ueno N. A human case of Echinostoma cinetorchis. Tokyo Iji Shinshi 1930; 2: 1326-1328 (in Japanese).
  4. Takahashi S, Ishii T, Ueno N. The second human case of Echinostoma cinetorchis and a case of tapeworm in man. Tokyo Iji Shinshi 1930; 2: 1326-1327 (in Japanese).
  5. Kawahara S, Yamamoto E. Human cases of Echinostoma cinetorchis. Tokyo Iji Shinshi 1933; 2: 1794-1796 (in Japanese).
  6. Takahashi S. The life cycle of Echinostoma cinetorchis and Echinostoma macrorchis, particularly their first and second intermediate hosts. Fukuoka Ika Daigaku Zasshi 1927; 20: 712-723 (in Japanese).
  7. Komiya Y. Metacercariae in Japan and adjacent territories. Section II. Special treatise. Progr Med Parasitol Japan 1965; 2: 1-328.
  8. Seo BS, Cho SY, Hong ST, Hong SJ, Lee SH. Studies on parasitic helminths of Korea. 5. Survey on intestinal trematodes of house rats. Korean J Parasitol 1981; 19; 131-136. https://doi.org/10.3347/kjp.1981.19.2.131
  9. Li WM, Barnes T, Lee CH. Endoribonucleases-enzymes gaining spotlight in mRNA metabolism. FEBS J 2010; 277: 627-641. https://doi.org/10.1111/j.1742-4658.2009.07488.x
  10. Bennett JR. Departments-leading the way-ribonuclease H: a tool in the battle against leishmaniasis. Chem Innov 2001; 31: 3-4.
  11. Parniak MA, Min KL, Budihas SR, Le Grice SF, Beutler JA. A fluorescence-based high-throughput screening assay for inhibitors of human immunodeficiency virus-1 reverse transcriptase-associated ribonuclease H activity. Anal Biochem 2003; 322: 33-39. https://doi.org/10.1016/j.ab.2003.06.001
  12. Nowak E, Miller JT, Bona MK, Studnicka J, Szczepanowski RH, Jurkowski J, Le Grice SF, Nowotny M. Ty3 reverse transcriptase complexed with an RNA-DNA hybrid shows structural and functional asymmetry. Nat Struct Mol Biol 2014; 21: 389-396. https://doi.org/10.1038/nsmb.2785