Journal of the Korean Society of Marine Environment & Safety (해양환경안전학회지)

The Korean Society of Marine Environment and safety (해양환경안전학회)
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Limiting Nutrient Based on Alkaline Phosphatase Activity in the Frontal Area of the Southern Sea, Korea

춘계 남해 전선역에서 알칼리 인산분해 효소를 통한 제한 영양염의 평가

  • Oh, Seok Jin (Department of Oceanography, Pukyong National University) ;
  • Jang, Minik (Department of Oceanography, Pukyong National University) ;
  • Nam, Ki Taek (Department of Oceanography, Pukyong National University) ;
  • Kim, Seok-Yun (Department of Oceanography, Pukyong National University)
  • Received : 2017.11.22
  • Accepted : 2017.12.28
  • Published : 2017.12.31
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Abstract

We estimated the limiting nutrient and DIP limiting history based on alkaline phosphatase (APase) activity during the spring of 2017 in the Southern Sea, Korea. In the frontal area, concentration of dissolved inorganic phosphorus (DIP), dissolved inorganic nitrogen (DIN): DIP ratio and Chlorophyll a (Chl-a) were < $0.2{\mu}M$, 23.2 and $2.2{\mu}g/L$, respectively, indicating high productivity despite DIP limiting. The relationship between APase and DIP indicates that the study area had limited DIP because of a strongly reverse correlation (r= -0.81; P<0.001). Relationship between APase and Chl-a (r=0.61, p<0.001) also indicated that APase may have been induced by phytoplankton (ca. 60 %) and bacteria (ca. 40 %). In DIP limiting history in this study area, frontal area and non-frontal areas might have induced long-term DIP limitation and the recent relief from DIP-limitation, respectively, based on distributions of dissolved APase and particulate APase. Thus, these results suggest that by measuring the enzyme that hydrolyzes organic matter such as APase in frontal area, it is possible to estimate temporal and spatial characteristics of limiting nutrient, thereby improving our understanding of biogeochemistry cycles.

2017년 추계에 남해 전선역을 파악하고, 알칼리 인산분해 효소(Alkaline Phosphatase; APase) 활성을 이용하여 제한 영양염과 제한 영양염의 시간적인 변화를 평가하였다. 전선역이 형성된 인근해역의 경우, 용존무기인(dissolved inorganic phosphorus; DIP)의 농도와 용존무기질소(dissolved inorganic nitrogen; DIN): DIP 비가 각각 $0.2{\mu}M$ 이하와 최대 23.2로, DIP가 제한된 환경임에도 불구하고 Chlorophyll a(Chl.-a)가 $0.2{\mu}g/L$로 높은 생물생산력을 보였다. APase와 DIP는 중요한 역의 상관관계(r = -0.81; P<0.001)를 보여, DIP가 제한되어진 해역임을 알 수 있었으며, APase와 Chl-a 관계는 APase의 60 %가 식물플랑크톤, 40 %가 박테리아 기원인 것으로 평가되었다. 용존태 APase와 입자태 APase의 분포로부터 전선역은 장기간 DIP가 제한된 해역이며, 그 외의 해역은 최근에 DIP 제한이 해소된 것으로 판단되었다. 따라서 전선역에서 APase와 같이 가수분해효소의 측정은 제한 영양염의 시공간적인 변화 특성을 평가할 수 있으며, 전선역에서 생지화학 순환의 이해를 높일 수 있을 것으로 생각된다.

Keywords

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