• Title/Summary/Keyword: Alkaline phosphatase

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Optimal Production of Thermostable Alkaline Phosphatase from Thermus caldophilus GK24 (Thermus caldophilus GK24로부터 내열성 alkaline phosphatase의 최적생산)

  • Kim, You-Jin;Chun, Myung-Sook;Kim, Hyun-Kyu;Kwon, Suk-Tae
    • Applied Biological Chemistry
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    • v.38 no.5
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    • pp.376-381
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    • 1995
  • Thermus caldophilus GK24 was selected as sources of thermostable alkaline phosphatase from a survey of extreme thermophile. T. caldophilus GK24 was tested for production of alkaline phosphatase by addition of various concentration of sodium glutamate, bactotryptone, glucose and yeast extract to basal salts. Sodium glutamate was found to be effective for the alkaline phosphatase induction. The optimal induction medium for production of alkaline phosphatase involves the addition of 0.3% sodium glutamate, 0.2% bactotryptone and 0.5% glucose to basal salts. The activity of the enzyme in optimal induction medium increased nearly 6-fold/ml than basal medium and 27.5-fold/ml than standard medium. T. caldophilus GK24 alkaline phosphatase was found to be inducible. When starved of inorganic phosphate, T. caldophilus GK24 produces the enzyme alkaline phosphatase. The addition of inorganic phosphate to growth medium had a repressive effect on enzyme synthesis.

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A Study on the Alkaline Phosphatase Activity in the Digestive Tracts of Fishes (魚類消化管의 Alkaline Phosphatase 活性에 관한 硏究)

  • 하재청;김국찬
    • The Korean Journal of Zoology
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    • v.17 no.4
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    • pp.167-176
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    • 1974
  • The authors have studied the distribution of alkaline phosphatase in the pharynx, esophagus, stomach (or intestinal bulb), anterior and posterior portions of intestine of three kinds of fishes. The results obtained are as follows: 1. Alkaline phosphatase activity of basal cells in the pharyngeal epithelium of loach and snakehead fish showed moderately positive reaction, and basal cells in the esophagel epithelium of loach and eel showed also moderately positive reaction. 2. Goblet cells of pharynx, esophagus, intestinal bulb and intestinal mucosa, and gastric glandular cells of the above fishes showed negative reaction for alkaline phosphatase. 3. Strongly positive reaction for alkaline phosphatase was observed at both intestinal bulb and the free border of intestinal epithelium, but weak positive reaction at the free border of posterior portion of loach intestine.

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Alkaline Phosphatase Activity in the Developing Pronephros and Mesonephros of the Frog Bombina orientalis (발생중의 무당개구리 前賢 및 中賢의 Alkaline Phosphatase활성)

  • Jae Chung Hah
    • The Korean Journal of Zoology
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    • v.17 no.4
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    • pp.177-184
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    • 1974
  • The cobalt capture method of Gomori's modified technique(Gomori, 1952) was employed to study the histochemistry of the developing frog kidney. Alkaline phosphatase activity was observed in association with the brush borders of pronephros and mesonephros. By the stage of transition from larva to tadpole alkaline phosphatase activity was gradually increased in the brush border of pronephros, and as the pronephros begun to degenerate the enzyme activity was decreased and disappeared. By the time of maximum development of the pronephros the mesonephros began to develop and alkaline phosphatase activity of the mesonephric tubules showed highly positive throughout the stage of metamorphosis. No activity was observed in association with the collecting tubules and ductal elements.

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The effects of continuous and intermittent compressive pressure on alkaline phosphatase activity of MC3T3-E1 cells (지속적 및 간혈적 가압력이 MC3T3-E1 세포의 Alkaline phosphatase 활성도에 미치는 영향)

  • Song, Hye-Seob;Kyung, Hee-Moon
    • The korean journal of orthodontics
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    • v.26 no.4
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    • pp.449-454
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    • 1996
  • The propose of this study was to evaluate the difference of cellular activity dependent on intermittent compressive force by determining the alkaline phosphatase activity. Alkaline phosphatase activity was measured on control and experimental groups every 24, 48, 72hours. Experimental groups consisted of continous and intermittent compressive group which were compressed by $300gm/cm^2$ of diaphram pump. The intermittent compressive group was connected by timer which was worked on 10 minutes an off 10 minutes. The results were as follows; 1. The alkaline phosphatase activity between control and experimental groups showed not significant difference at compressed 24 hours. 2. The alkaline phosphatase activity of experimental groups were more increased than control group at compressed 48 hours. 3. The alkaline phosphatase activity of intermittent compressive group showed significant increased to control group. Whereby continuous compressive group showed not significant difference to control at 72 hours. 4. The alkaline phosphatase activity of intermittent compressive group were stringly increased than continuous compressive groups. 5. Between experimental groups and control group no other morphologic changes were detected by microscopic findings.

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Studies on the Alkaline Phosphatase of Pekin Duck: I. Some Properties of Liver Alkaline Phosphatase

  • Kang, Shin-Sung;Park, Tae-Kyu
    • The Korean Journal of Zoology
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    • v.25 no.2
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    • pp.71-80
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    • 1982
  • Some properties of alkaline phosphatase, partially purified from the liver of the local Pekin duck, Anas platyrhynchos, were investigated with the following results. 1. Gel-filtration of the duck liver extract indicated the presence of two molecular-weight species of alkaline phosphatase. 2. Electrophoresis of both enzyme preparations suggests the presence of two molecular forms of alkaline phosphatase with different physicochemical characteristics. 3. The liver alkaline phosphatase has an optimum pH of 9.0, and is further activated by $Mg^2+$ but not by $Ca^2+$. 4. The enzyme was relatively heat-labile, and was competitively inhibited by phosphate ions, but uncompetitively inhibited by L-phenylalanine. 5. These results are discussed in comparison with the properties of human and other animal alkaline phosphatases.

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Bone Alkaline Phosphatase Levels in Serum of Normal Dogs (정상적인 개에서의 Serum Bone Alkaline Phosphatase의 활성치)

  • 조성진;김남수;최인혁
    • Journal of Veterinary Clinics
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    • v.14 no.1
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    • pp.65-69
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    • 1997
  • The bone alkaline phosphatase(BALP) in 130 sera of normal dogs were assayed according to the lectin precipitation method of Rosalki and Foo. The serum BALP activities showed a wide variation as $23.27({\pm}14.73)$ IU/L in young dogs from 6weeks to 12 months old and were lower in magnitude as $9.24({\pm}3.36)$ IU/L in elder dogs from 1 years to 6years old. The serum BALP activities in normal dogs were no significant correlation in sex.

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Activity of Alkaline Phosphatase from the Mosquito, Culex pipiens pallens (홍모기(Culex pipiens pallens)의 난성숙 과정 중 alkaline phosphase의 활성)

  • 이영수;이승훈;박영민;성기창
    • The Korean Journal of Zoology
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    • v.36 no.3
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    • pp.425-432
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    • 1993
  • Alkaline phosphatase from Culex pipiens pallens was examined to determine the optimal assay condition and to assay the activity during ovarian development. The activity of alkaline phosphatase in a male and a nongravid female continuously were declined after eclosion. But by the stimulus of a blood meal, the enzyme activity was increased dramatically. At 30 hr. after a blood meal, the maximal activity was reached and then declined. And after 48 hr. after a blood meal, the second activity increase was revealed. This second increase was maintained up to oviposition. The first activity increase was revealed in the midgut and the second increase was done in the ovary to assay the organ distribution of alkaline phosphatase. In electrophresis data, it was shown 5 isozyme bands, ALP-1 and ALP-2 in the ovary, ALP-3 in the thorax and the midgut, and ALP-4 and ALP-5 in the thorax, the fatbody and the midgut in crude extract at 30 hr. after a blood meal. One the same ovary pattern were shown at 72 hr. after a blood meal.

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Histochemical Distribution of Glycogen and Alkaline Phosphatase in Placenta of Golden Hamster (Golden Hamster 태반(胎盤)의 Glycogen 및 Alkaline Phosphatase의 조직화학적분포(組織化學的分布))

  • Lee, Cha Soo
    • Korean Journal of Veterinary Research
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    • v.15 no.2
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    • pp.233-240
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    • 1975
  • Glycogen and alkaline phosphatase were studied histochemically in the placenta of the golden hamster (Mesocricetus auratus). The results were summarized as followings: 1. The histochemical distribution of glycogen and alkaline phosphatase was described in the various cells of both the placenta and uterus from implantation (5 days past coitum) parturition. 2. Glycogen appeared in the extraembryonic ectorm of the implanting ovum and increased gradually in the ectoplacental cone with the development of the placenta. 3. Glycogen granules began to accumulate in the labylinthine trophoblast of chorioallantoic placenta at 10.5 days and in the endodermal cells the visceral yolk sac sac at 10 days, respectively, and in these both cells glycogen increased gradually until the 13 days post coitum and after this time decreased progressively. 4. Alkaline phosphatase activity was high in the labyrinth, junctional zone and decidua, but was not observed in the yolk sac and trophospongium.

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Serum Alkaline Phosphatase Activity of Korean Cattle (한우(韓牛)의 혈청(血淸) alkaline phosphatase 활성도(活性度)에 관(關)하여)

  • Yong, Mahn J.;Nam, Tchi C.;Cheong, Chang K.
    • Korean Journal of Veterinary Research
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    • v.11 no.2
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    • pp.141-143
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    • 1971
  • The serum alkaline phosphatase activity was determined with 64 Korean cattles in different sexe and age groups, using p-nitrophenylphosphate as the substrate. The results obtained were as follows: 1. The mean value of serum alkaline phosphatase activity in Korean cattle was $1.78{\pm}0.21$ Bessey-Lowry units/ml, and $1.71{\pm}0.21$ units/ml. in female and $1.86{\pm}0.21$ units/ml. in male, respectively. 2. The serum alkaline phosphatase activity decreased with age from 4 years group. 3. No statistical significance was recognized among the sexes, but difference among the age groups was found to be highly significant.

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Mass Spectrometry in the Determination of Glycosylation Site and N-Glycan Structures of Human Placental Alkaline Phosphatase

  • Solakyildirim, Kemal;Li, Lingyun;Linhardt, Robert J.
    • Mass Spectrometry Letters
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    • v.9 no.3
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    • pp.67-72
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    • 2018
  • Alkaline phosphatase (AP) is a membrane-bound glycoprotein that is widely distributed in the plasma membrane of cells of various organs and also found in many organisms from bacteria to humans. The complete amino acid sequence and three-dimensional structure of human placental alkaline phosphatase have been reported. Based on the literature data, AP consists of two presumptive glycosylation sites, at Asn-144 and Asn-271. However, it only contains a single occupied N-linked glycosylation site and no occupied O-linked glycosylation sites. Hydrophilic interaction chromatography (HILIC) has been primarily employed for the characterization of the glycan structures derived from glycoproteins. N-glycan structures from human placental alkaline phosphatase (PLAP) were investigated using HILIC-Orbitrap MS, and subsequent data processing and glycan assignment software. 16 structures including 10 sialylated N-glycans were identified from PLAP.