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Antimutagenic Effect of Mulberry Leaf Extract

뽕잎 추출물의 돌연변이 억제효과

  • Lim, Bum Hyuk (Department of Industrial and System Engineering, KAIST) ;
  • Park, Chang Gyun (Department of Industrial Plant Science and Technology, Chungbuk National University) ;
  • Cho, Hyun Ki (Department of Industrial Plant Science and Technology, Chungbuk National University) ;
  • Lim, Heung Bin (Department of Industrial Plant Science and Technology, Chungbuk National University)
  • 임범혁 (한국과학기술원) ;
  • 박창균 (충북대학교 응용생명공학부) ;
  • 조현기 (충북대학교 응용생명공학부) ;
  • 임흥빈 (충북대학교 응용생명공학부)
  • Received : 2017.06.26
  • Accepted : 2017.08.16
  • Published : 2017.08.30

Abstract

Background: The present study was carried out to asses whether mulberry leaves (MLs) have the potential to inhibit the mutagenic effect of cigarette smoke condensates (CSCs). Methods and Results: ML powder was extracted with 70% ethanol, and a yield of 35.1% by weight was obtained. The 70% ethanol extract of ML was further extracted sequentially using diethyl ether, chloroform, butanol, dichloromethane and water. The crude 70% ethanol extract of MLs and its solvent fractions did not show any mutagenic effect when tested at concentrations up to 1 mg/plate against Salmonella typhimurium TA98. In contrast, the crude 70% ethanol extract showed an inhibitory activity against the mutagenicity of CSCs in the presence of S-9 mixture. Among the solvent fractions, the diethyl ether fraction showed the highest inhibitory activity, which increased in a dose-dependent manner, inhibiting mutagenesis by approximately 97.1% at a concentration of 1 mg/plate. Conclusions: In this study, we found that a crude 70% ethanol extract of MLs and the diethyl ether fraction themselves are potentially not mutagenic, but inhibit the mutagenic effect of CSCs.

Keywords

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