Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Comparative Genomic Analysis of Lactobacillus plantarum GB-LP1 Isolated from Traditional Korean Fermented Food

  • Yu, Jihyun (Department of Agricultural Biotechnology and Research Institute of Population Genomics, Seoul National University) ;
  • Ahn, Sojin (Interdisciplinary Program in Bioinformatics, Seoul National University) ;
  • Kim, Kwondo (Interdisciplinary Program in Bioinformatics, Seoul National University) ;
  • Caetano-Anolles, Kelsey (Department of Agricultural Biotechnology and Research Institute of Population Genomics, Seoul National University) ;
  • Lee, Chanho (Genebiotech Co. Ltd.) ;
  • Kang, Jungsun (Genebiotech Co. Ltd.) ;
  • Cho, Kyungjin (Genebiotech Co. Ltd.) ;
  • Yoon, Sook Hee (Department of Agricultural Biotechnology and Research Institute of Population Genomics, Seoul National University) ;
  • Kang, Dae-Kyung (Department of Animal Resources Science, Dankook University) ;
  • Kim, Heebal (Department of Agricultural Biotechnology and Research Institute of Population Genomics, Seoul National University)
  • Received : 2017.04.06
  • Accepted : 2017.06.13
  • Published : 2017.08.28
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Abstract

As probiotics play an important role in maintaining a healthy gut flora environment through antitoxin activity and inhibition of pathogen colonization, they have been of interest to the medical research community for quite some time now. Probiotic bacteria such as Lactobacillus plantarum, which can be found in fermented food, are of particular interest given their easy accessibility. We performed whole-genome sequencing and genomic analysis on a GB-LP1 strain of L. plantarum isolated from Korean traditional fermented food; this strain is well known for its functions in immune response, suppression of pathogen growth, and antitoxin effects. The complete genome sequence of GB-LP1 is a single chromosome of 3,040,388 bp with 2,899 predicted open reading frames. Genomic analysis of GB-LP1 revealed two CRISPR regions and genes showing accelerated evolution, which may have antibiotic and antitoxin functions. The aim of the present study was to predict strain specific-genomic characteristics and assess the potential of this new strain as lactic acid bacteria at the genomic level using in silico analysis. These results provide insight into the L. plantarum species as well as confirm the possibility of its utility as a candidate probiotic.

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