Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Antiamoebic Activity of Petiveria alliacea Leaves and Their Main Component, Isoarborinol

  • Zavala-Ocampo, Lizeth M. (Posgrado en Biomedicina Molecular, Escuela Nacional de Medicina y Homeopatia, Instituto Politecnico Nacional) ;
  • Aguirre-Hernandez, Eva (Laboratorio de Fitoquimica, Facultad de Ciencias, Universidad Nacional Autonoma de Mexico) ;
  • Perez-Hernandez, Nury (Posgrado en Biomedicina Molecular, Escuela Nacional de Medicina y Homeopatia, Instituto Politecnico Nacional) ;
  • Rivera, Gildardo (Laboratorio de Biotecnologia Farmaceutica, Centro de Biotecnologia Genomica, Instituto Politecnico Nacional) ;
  • Marchat, Laurence A. (Posgrado en Biomedicina Molecular, Escuela Nacional de Medicina y Homeopatia, Instituto Politecnico Nacional) ;
  • Ramirez-Moreno, Esther (Posgrado en Biomedicina Molecular, Escuela Nacional de Medicina y Homeopatia, Instituto Politecnico Nacional)
  • Received : 2017.05.02
  • Accepted : 2017.06.13
  • Published : 2017.08.28
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Abstract

Petiveria alliacea L. (Phytolacaceae) is a medicinal plant with a broad range of traditional therapeutic properties, including the treatment of dysentery and intestinal infections caused by protozoan parasites. However, its effects against Entamoeba histolytica have not been reported yet. We investigated the antiamoebic activity present in the leaves of P. alliacea Antiamoebic activity was evaluated in methanolic and aqueous extracts, as well as in the hexanic, methanolic, and EtOAc fractions. The P. alliacea methanolic extract showed a better antiamoebic activity than the aqueous extract with an $IC_{50}=0.51mg/ml$. Likewise, the hexanic fraction was the most effective fraction, showing a dose-dependent activity against E. histolytica, with an $IC_{50}=0.68mg/ml$. Hexanic subfraction 12-19 showed the highest antiamoebic activity at 0.8 mg/ml, producing 74.3% growth inhibition without any toxicity in mammal cells. A major component in subfraction 12-19 was identified as isoarborinol, which produced 51.4% E. histolytica growth inhibition at 0.05 mg/ml without affecting mammal cells. The P. alliacea leaf extract has antiamoebic activity that can be attributed to a major metabolite known as isoarborinol.

Keywords

References

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