한국발생생물학회지:발생과생식 (Development and Reproduction)

  • 제21권4호
  • /
  • Pages.361-370
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  • 2017
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  • 2465-9525(pISSN)
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  • 2465-9541(eISSN)
한국발생생물학회 (The Korean Society of Developmental Biology)
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Immune Response to Koi Herpesvirus (KHV) of Koi and Koi × Red Common Carp (Cyprinus carpio)

  • Hwang, Ju-ae (Inland Aquaculture Research Center, National Institute of Fisheries Science (NIFS)) ;
  • Kim, Jung Eun (Inland Aquaculture Research Center, National Institute of Fisheries Science (NIFS)) ;
  • Kim, Hyeong-su (Inland Aquaculture Research Center, National Institute of Fisheries Science (NIFS)) ;
  • Lee, Jeong-Ho (Inland Aquaculture Research Center, National Institute of Fisheries Science (NIFS))
  • 투고 : 2017.10.10
  • 심사 : 2017.12.13
  • 발행 : 2017.12.31
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초록

Koi herpesvirus (KHV), also known as Cyprinid herpes virus 3 (Cyprinid 3) is lethal disease in common carp and koi (Cyprinus carpio). Two different groups (KK and RK) were infected KHV by intraperitoneal injection. Fish for gene expression analysis were sampled at 0 h, 12 h, 24 h, 48 h and 72 h post infection (p.i). The results showed that two immune related gene, Interferons (INFs) ${\alpha}{\beta}$ and Interleukin (IL)-12 p35 induced a high response in RK. The IL-12 p35 cytokine and Toll-like receptor (TLR) 9 were significantly high expressed on 48 h post infection (p.i) in RK as compared to the KK. The histopatological examination reveals focal necrosis in liver and infiltrate of lymphocytes in spleen of KK as compared to the RK. In immunohistochemistry analysis, the KHV protein high expressed in the infected kidney cell and slenocyte of KK. Therefore, the expression of IL-12 p35, IFN ${\alpha}{\beta}$ and TLR 9 may provide a potentially genes related with KHV resistance in Koi and red common carp ${\times}$ koi.

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참고문헌

  1. Adamek M, Rakus KL, Chyb J, Brogden G, Huebner A, Irnazarow I, Steinhagen D (2012) Interferon type I responses to virus infections in carp cells: In Vitro studies on Cyprinid herpesvirus 3 and Rhabdovirus carpio infections. Fish Shellfish Immunol 33:482-493. https://doi.org/10.1016/j.fsi.2012.05.031
  2. Adamek M, Rakus KL, Brogden G, Matras M, Chyb J, Hirono I, Kondo H, Aoki T, Irnazarow I, Steinhagen D (2014) Interaction between type I interferon and Cyprinid herpesvirus 3 in different genetic lines of common carp (Cyprinus carpio L.). Dis Aquat Org 111:107-118. https://doi.org/10.3354/dao02773
  3. Adamek M, Syakuri H, Harris S, Rakus KL, Brogden G, Matras M, Irnazarow I, Steinhagen D (2013) Cyprinid herpesvirus 3 infection disrupts the skin barrier of common carp (Cyprinus carpio). Vet Microbiol 162:456-470. https://doi.org/10.1016/j.vetmic.2012.10.033
  4. Aggad D,Mazel M, Boudinot P, Mogensen KE, Hamming OJ, Hartmann R, other authors (2009) The two groups of zebrafish virus-induced interferons signal via distinct receptors with specific and shared chains. J Immunol 183:3924-3931. https://doi.org/10.4049/jimmunol.0901495
  5. Aoki T, Hirono I, Kurokawa K, Fukuda H, Nahary R, Eldar A (2007) Genome sequences of three koi herpesvirus isolates representing the expending distribution of an emerging disease threatening koi and common carp worldwide. J Virol 81:5058-5065. https://doi.org/10.1128/JVI.00146-07
  6. Bondat-Reantaso MG, Sunarto A, Subasinghe RP (2007) Managing the koi herpesvirus disease outbreak in Indonesia and the lessons learned. Dev Biol (Basel) 129:21-8.
  7. Dixon PF, Joiner CL, Way K, Reese RA, Jeney G, Jeney Z (2009) Comparison of the resistance of selected families of a common carp, Cyprinus carpio L., to koi herpesvirus: preliminary study. J. Fish Dis 32:1035-1039. https://doi.org/10.1111/j.1365-2761.2009.01081.x
  8. Gilad O, Yun S, Andree KB, Adkison MA, Zlotkin A, Bercovier H, Eldar A, Hedrick RP (2002) Initial characteristics of koi herpesvirus and development of a polymerase chain reaction assay to detect the virus in koi, Cyprinus carpio koi. Dis Aquat Org 48:101-108. https://doi.org/10.3354/dao048101
  9. Gomez DK, Joh SJ, Jang H, Shin SP, Choresca CH, Han JE, Kim JH, Jun JW, Park SC (2011) Detection of koi herpesvirus (KHV) from koi (Cyprinus carpio koi) broodstock in South Korea. Aquaculture 311: 42-47. https://doi.org/10.1016/j.aquaculture.2010.11.021
  10. Hedrick RP, Gilad O, Yun S, Spangenberg JV, Marty GD, Nordhausen RW, Kebus MJ, Bercovier H, Eldar A (2000) A herpesvirus associated with mass mortality of juvenile and adult koi, a strain of a common carp. J Aquat Anim Health 12:44-57. https://doi.org/10.1577/1548-8667(2000)012<0044:AHAWMM>2.0.CO;2
  11. Hedrick RP, Waltzek TB, McDowell TS (2006) Susceptibility of koi carp, common carp, goldfish and goldfish ${\times}$ common carp hybrids to cyprinid herpesvirus-2 and herpesvirus-3. J Aquat Anim Health 18:26-34. https://doi.org/10.1577/H05-028.1
  12. Hines RS, Wohlfarth G, Moav R, Hulata G (1974) Genetic differences in susceptibility to two diseases among strains of common carp. Aquaculture 3:187-97. https://doi.org/10.1016/0044-8486(74)90112-4
  13. Kongchum P, Palti Y, Hallerman EM, Hulata G, David L (2010) SNP discovery and development of genetic markers for mapping innate immune response genes in common carp (Cyprinus carpio). Fish & Shellfish Immunology 29:356-361. https://doi.org/10.1016/j.fsi.2010.04.013
  14. Kongchum P, Sandel E, Lutzky S, Hallerman EM, Hulata G, David L, Palti Y (2011) Association between IL-10a single nucleotide polymorphism and resistance to cyprinid herpesvirus-3 infection in common carp (Cyprinus carpio). J Aquacult 315:417-421. https://doi.org/10.1016/j.aquaculture.2011.02.035
  15. Li XS, Liu ZZ, Zhao XJ, Ma XQ, Feng W, Wang CH (2011) Major histocompatibility complex DAB allele polymorphism and its association with resistance/susceptibility to Aeromonas hydrophila in "whole red" color patterns of Cyprinus carpio var. color. J Fish Sci China 35:1293-1301.
  16. McGeoch DJ, Rixon FR, Davison AJ (2006)Topics in herpesvirus genomics and evolution. Virus Res 117:90-104. https://doi.org/10.1016/j.virusres.2006.01.002
  17. OIE (2013) In: Manual of Diagnostic Tests for Aquatic Animals.
  18. Ouyang P, Rakus K, Boutier M, Reschner A, Leroy B, Ronsmans M, Fournier G, Scohy S, Costes B, other authors (2013). The IL-10 homologue encoded by cyprinid herpesvirus 3 is essential neither for viral replication in vitro nor for virulence in vivo. Vet Res 1:44-53.
  19. Pikarsky E, Ronen A, Abramowitz J, Levavi-Sivan B, others (2004) Pathogenesis of acute viral disease induced in fish by carp interstitial nephritis and gill necrosis virus. J Virol 78:9544-9551. https://doi.org/10.1128/JVI.78.17.9544-9551.2004
  20. Purcell MK, LaPatra SE, Woodson JC, Kurath G, Winton JR (2010) Early viral replication and induced or constitutive immunity in rainbow trout families with differential resistance to Infectious hematopoietic necrosis virus (IHNV). Fish Shellfish Immunol 28:98-105. https://doi.org/10.1016/j.fsi.2009.10.005
  21. Rakus KL, Irnazarow I, Adamek M, Palmeira L, Kawana Y, Hirono I, Kondo H, Matras M, Steinhagen D, Flasz B, Brogden G, Vanderplasschen A, Aoki T (2012) Gene expression analysis of common carp (Cyprinus carpio L.) lines during Cyprinid herpesvirus 3 infection yields insights into differential immune responses. Dev Comp Immunol 37:65-76. https://doi.org/10.1016/j.dci.2011.12.006
  22. Rakus KL, Wiegertjes GF, Admek M, Siwicki AK, Lepa A, Irnazaro I (2009) Resistance of common carp (Cypprinus carpio L.) to Cyprinid herpesvirus-3 is influenced by major histocompatibility (MH) class II B gene polymorphism. Fish shellfish Immunol 26:737-743. https://doi.org/10.1016/j.fsi.2009.03.001
  23. Robertsen B (2006) The interferon system of teleost fish. Fish Shellfish Immuol 20:172-191. https://doi.org/10.1016/j.fsi.2005.01.010
  24. Samuel CE (2001) Antiviral actions of interferons Clin Microbiol Rev 14 (4), pp. 778-809 https://doi.org/10.1128/CMR.14.4.778-809.2001
  25. Sano M, Ito T, Kurita J, Yanai T, Watanabe N, Satoshi M, et al. (2004) First detection of koi herpesvirus in cultured common carp Cyprinus carpio in Japan. Fish Pathol 39:165-167. https://doi.org/10.3147/jsfp.39.165
  26. Shapira Y, Magen Y, Zak T, Ktler M, Hulata G, Levavi-Sivan B (2005) Differential resistance to koi herpes virus (KHV)/carp interstitial nephritis and gill necrosis virus (CNGV) among common carp (Cyprinus carpio L.) strains and crossbreds. Aquaculture 245:1-11. https://doi.org/10.1016/j.aquaculture.2004.11.038
  27. Sunarto A, McColl KA (2015) Expression of immune-related genes of common carp during cyprinid herpesvirus 3 infection. Dis Aquat Organ 9:127-135.
  28. Trinchieri G (2003) Interleukin-12 and the regulation of innate resistance and adaptive immunity. Nat Rev Immunol 3:133-146. https://doi.org/10.1038/nri1001
  29. Tu C, Weng MC, Shiau JR, Lin SY (2004) Detection of koi herpesvirus in koi Cyprinus carpio in Taiwan. Fish Pathol 39:109-110. https://doi.org/10.3147/jsfp.39.109
  30. Verrier ER, Langevin C, Tohry C, Houel A, others (2012) Genetic resistance to rhabdovirus infection in teleost fish is paralleled to the derived cell resistance status. PLoS ONE 7:e33935. https://doi.org/10.1371/journal.pone.0033935
  31. Wang T, Husain M (2014) The expanding repertoire of the IL-12 cytokine family in teleost fish: Identification of three paralogues each of the p35 and p40 genes in salmonids, and comparative analysis of their expression and modulation in Atlantic salmon Salmo salar. Dev Comp Immunol 46:194-207. https://doi.org/10.1016/j.dci.2014.04.008
  32. Watford WT, Moriguchi M, Morinobu A, O'Shea JJ (2003) The biology of IL-12: coordinating innate and adaptive immune responses. Cytokine Growth Factor Rev 14:361-368. https://doi.org/10.1016/S1359-6101(03)00043-1
  33. Zhang S, Wang Q (2008) Factors determining the formation and release of bioactive IL-12: regulatory mechanisms for IL-12p70 synthesis and inhibition. Biochem Biophys Res Commun 372:509-512. https://doi.org/10.1016/j.bbrc.2008.05.081
  34. Zhao XJ, Liu ZZ, Li XS, Wang Y, Wang CH (2012) Major histocompatibility complex class II B allele polymorphism and its association with resistance/susceptibility to Aeromonas hydrophila in the "whole white" color pattern of Cyprinus carpio var. color. J Fish Sci China 19:399-407.