Mass Spectrometry Letters

Korean Society for Mass Spectrometry (한국질량분석학회)
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The Influence of Mixed Solvents Volatility on Charge State Distribution of Peptides During Positive Electrospray Ionization Mass Spectrometry

  • Nielsen, Birthe V. (University of Greenwich, Faculty of Engineering and Science, Chatham Maritime) ;
  • Abaye, Daniel A. (University of Greenwich, Faculty of Engineering and Science, Chatham Maritime) ;
  • Nguyen, Minh T.L. (University of Greenwich, Faculty of Engineering and Science, Chatham Maritime)
  • Received : 2017.04.24
  • Accepted : 2017.06.03
  • Published : 2017.06.30
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Abstract

Understanding the mechanisms that control and concentrate the observed electrospray ionisation (ESI) response from peptides is important. Controlling these mechanisms can improve signal-to-noise ratio in the mass spectrum, and enhances the generation of intact ions, and thus, improves the detection of peptides when analysing mixtures. The effects of different mixtures of aqueous: organic solvents (25, 50, 75%; v/v): formic acid solution (at pH 3.26) compositions on the ESI response and charge-state distribution (CSD) during mass spectrometry (MS) were determined in a group of biologically active peptides (molecular wt range 1.3 - 3.3 kDa). The ESI response is dependent on type of organic solvent in the mobile phase mixture and therefore, solvent choice affects optimal ion intensities. As expected, intact peptide ions gave a more intense ESI signal in polar protic solvent mixtures than in the low polarity solvent. However, for four out of the five analysed peptides, neither the ESI response nor the CSD were affected by the volatility of the solvent mixture. Therefore, in solvent mixtures, as the composition changes during the evaporation processes, the $pK_b$ of the amino acid composition is a better predictor of multiple charging of the peptides.

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References

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