Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Overexpression of rice NAC transcription factor OsNAC58 on increased resistance to bacterial leaf blight

전사인자 OsNAC58 과발현을 통한 벼 흰잎마름병 저항성 증진 벼

  • Park, Sang Ryeol (Gene Engineering Div., National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Hye Seon (Gene Engineering Div., National Academy of Agricultural Science, Rural Development Administration) ;
  • Lee, Kyong Sil (Gene Engineering Div., National Academy of Agricultural Science, Rural Development Administration) ;
  • Hwang, Duk-Ju (Gene Engineering Div., National Academy of Agricultural Science, Rural Development Administration) ;
  • Bae, Shin-Chul (Gene Engineering Div., National Academy of Agricultural Science, Rural Development Administration) ;
  • Ahn, Il-Pyung (Gene Engineering Div., National Academy of Agricultural Science, Rural Development Administration) ;
  • Lee, Seo Hyun (Department of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University) ;
  • Kim, Sun Tae (Department of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University)
  • 박상렬 (농촌진흥청 국립농업과학원 농업생명자원부 유전자공학과) ;
  • 김혜선 (농촌진흥청 국립농업과학원 농업생명자원부 유전자공학과) ;
  • 이경실 (농촌진흥청 국립농업과학원 농업생명자원부 유전자공학과) ;
  • 황덕주 (농촌진흥청 국립농업과학원 농업생명자원부 유전자공학과) ;
  • 배신철 (농촌진흥청 국립농업과학원 농업생명자원부 유전자공학과) ;
  • 안일평 (농촌진흥청 국립농업과학원 농업생명자원부 유전자공학과) ;
  • 이서현 (부산대학교 식물생명과학과) ;
  • 김선태 (부산대학교 식물생명과학과)
  • Received : 2017.06.08
  • Accepted : 2017.06.20
  • Published : 2017.06.30
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Abstract

Bacterial blight in rice caused by Xanthomonas oryzae pv. oryzae (Xoo) greatly reduces the growth and productivity of this important food crop. Therefore, we sought to increase the resistance of rice to bacterial blight by using a NAC (NAM, ATAF, and CUC) transcription factor, one of the plant-specific transcription factors that is known to be involved in biotic/abiotic stress resistance. By isolating the OsNAC58 gene from rice and analyzing its biological functions related to Xoo resistance, phylogenetic analysis showed that OsNAC58 belongs to group III. To investigate the biological relationship between bacterial leaf blight (BLB) and OsNAC58 in rice, we constructed a vector for overexpression in rice and generated transgenic rice. The expression analysis resulting from use of RT-PCR showed that OsNAC58-overexpressed transgenic rice exhibited higher levels of OsNAC58 expression than wild types. Further, subcellular localization analysis using rice protoplasts showed that the 35S/OsNAC58-SmGFP fusion protein was localized in the nuclei. Thirteen OsNAC58-overexpressed transgenic rice lines, with high expression levels of OsNAC58, showed more resistant to Xoo than did the wild types. Together, these results suggest that the OsNAC58 gene of rice regulates the rice disease resistance mechanism in the nucleus upon invasion of the rice bacterial blight pathogen Xoo.

벼는 중요한 식량작물이며 지속적으로 벼흰잎마름병균, 도열병균, 잎집무늬마름병균, 바이러스 등 여러 병원균에 의해 수확량이 영향을 받고 있다. 이들 중 Xanthomonas oryzae pv. oryzae (Xoo)에 의해 유발되는 벼흰잎마름병은 세계 벼 재배지역에 발병하여 막대한 피해를 주고 있어 문제가 되고 있다. 따라서 생물적/비생물적 스트레스 저항성에 관여한다고 알려져 있는 식물 특이 전사인자 중의 하나인 NAC(NAM, ATAF, and CUC) 전사인자를 이용하여 벼의 벼흰잎마름병에 대한 저항성을 증진시키고자 하였다. 본 연구에서는 벼에서 NAC 전사인자 중 하나인 OsNAC58 유전자를 분리해 냈으며 아미노산 서열을 바탕으로 분석해 본 결과이 유전자는 5개의 NAC전사인자 group 중에서도 stress와 많은 관련이 있다고 알려진 group III에 속하였다. 또한 세포 내 위치를 확인하기 위해 GFP와 융합한 단백질을 이용해 조사해 본 세포 내에서도 핵에 위치하는 것으로 조사되었다. OsNAC58 유전자의 생물학적 기능 분석을 위해 이 유전자를 과발현시킨 벼 형질전환체를 만들었다. 동진벼를 기준으로 보다 발현이 높은 13개 계통을 선발하였으며, 이들 계통에 벼흰잎마름병균을 접종하여 병저항성을 검정한 결과 동진벼에 비해 벼흰잎마름병에 대한 저항성이 크게 증대함을 보였다. 이것은 벼의 OsNAC58 유전자가 벼흰잎마름병균 침입 시 숙주인 벼 핵 내에서 벼의 병저항성 기작을 조절하여 나타난 결과로 추정된다.

Keywords

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