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신규 Sporichthyaceae Bacterium Strain K-07 배양액의 피부장벽, 보습 및 항염증 활성

Novel Sporichthyaceae Bacterium Strain K-07 Skin Barrier, Moisturizing and Anti-inflammatory Activity

  • 투고 : 2017.05.02
  • 심사 : 2017.06.27
  • 발행 : 2017.06.30

초록

피부의 생태계는 미생물에게 다양한 형태의 서식처를 제공하며, 광범위한 미생물들이 살고 있다. 숙주인 사람은 이들과 공생관계를 이루고 있으며, 이들은 숙주에 많은 긍정적인 영향을 미친다. 피부에 분포하는 미생물들의 다양한 대사물질은 피부세포에 영향을 미치고, 피부장벽 기능, 노화방지 및 항염증에 광범위한 효능을 나타내는 것으로 알려져 있다. 본 연구에서는 사람의 피부에서 신규한 Sporichthyaceae bacterium strain K-07을 분리하였고, 해당 미생물의 16S rRNA 분석결과 Sporichthya속의 미생물과 상동성이 93.4%인 것으로 신규속(genus)으로 확인되었다. 그리고 신규로 분리된 K-07 배양액을 처리하였을 때, HaCaT cell에 어떤 변화가 나타나는지에 대한 분석을 실시하였다. 분리된 신규 미생물 strain K-07의 16S rRNA sequence 분석결과 상동성이 93.4% 이하로 확인되었고, 보고되지 않은 새로운 종으로 확인되었다. Filaggrin, cluadin1, claudin4, ${\alpha}SMase$, 및 CerS3 / HAS3 및 aquaporin3 / IL-6 및 TNF-${\alpha}$ / TSLP 및 TARC를 대상으로 strain K-07 배양액을 처리하여 변화를 관찰하였다. 그 결과 filaggrin, cluadin1, claudin4, ${\alpha}$SMase, 및 CerS3 / HAS3 및 aquaporin3에서는 음성 대조군 대비 우수한 증가 효과가 나타남을 확인하였다. 그리고 IL-6 및 TNF-${\alpha}$ / TSLP 및 TARC에 대해서도 우수한 억제능을 나타내는 것을 확인하였다. 결론적으로 신규 미생물 strain K-07 배양액은 피부 장벽활성 증진에 매우 효과적인 작용을 하며, 염증 억제에 우수한 효능을 나타내는 것으로 확인되므로 피부에 효과적인 소재로 사용될 수 있을 것이다.

The human skin is an ecosystem that provides habitat to various microorganisms. These comprise the skin microbiome and provide numerous benefits in addition to maintaining a symbiotic relation with the host. Various metabolites generated by the skin microbiome exert beneficial effects such as strengthening the skin barrier, and anti-aging and anti-inflammatory functions. In this study, we isolated a novel bacterium, designated Sporichthyacae strain K-07, from the human skin. Analysis of 16S rRNA gene sequences showed that the newly found bacterium shares 93.4% homology with the genus Sporichthya, thus corroborating the discovery of a novel genus. We further analyzed the effect of the novel strain in vitro, by treating HaCaT cells with bacterial metabolite products. Treatment resulted in changes in the mRNA expression levels of filaggrin, claudin1, claudin4, SMase, CERS3, HAS3, aquaporin3, IL-6, TNF-${\alpha}$, TSLP, and TARC. Specifically, the levels of filaggrin, claudin1, claudin4, SMase, CERS3, HAS3, and aquaporin3 were higher in strain K-07 metabolite product-treated cells than in control cells. These results showed that metabolite products of the novel strain K-07 enhanced the skin barrier and exert anti-inflammatory effects. Therefore, these metabolite products could be potentially used for treatment of skin conditions.

키워드

참고문헌

  1. K. Chiller, B. A. Selkin, and G. J. Murakawa, Skin microflora and bacterial infections of the skin, J. Investig. Dermatol. Symp. Proc., 6, 170 (2001). https://doi.org/10.1046/j.0022-202x.2001.00043.x
  2. D. N. Fredricks, Microbial ecology of human skin in health and disease, J. Investig. Dermatol. Symp. Proc., 6, 167 (2001). https://doi.org/10.1046/j.0022-202x.2001.00039.x
  3. R. R. Roth and W. D. James, Microbial ecology of the skin, Annu. Rev. Microbiol., 42, 441 (1988). https://doi.org/10.1146/annurev.mi.42.100188.002301
  4. W. C. Noble, Skin microbiology: coming of age, J. Med. Microbiol., 17, 1 (1984). https://doi.org/10.1099/00222615-17-1-1
  5. R. R. Roth and W. D. James, Microbiology of the skin: resident flora, ecology, infection, J. Am. Acad. Dermatol., 20, 367 (1989). https://doi.org/10.1016/S0190-9622(89)70048-7
  6. A. L. Cogen, V. Nizet, and R. L. Gallo, Skin microbiota: a source of disease or defence?, Br. J. Dermatol., 158, 442 (2008). https://doi.org/10.1111/j.1365-2133.2008.08437.x
  7. C. Callewaert, F. M. Kerckhof, M. S. Granitsioti, M. Van Gele, T. Van de Wiele, and N. Boon, Characterization of Staphylococcus and Corynebacterium clusters in the human axillary region, PLoS One, 8, e70538 (2013) https://doi.org/10.1371/journal.pone.0070538
  8. E. A. Grice and J. A. Segre, The skin microbiome, Nat. Rev. Microbiol., 9, 244 (2011). https://doi.org/10.1038/nrmicro2537
  9. A. Martin, M. A. Saathoff, F. Kuhn, H. Max, L. Terstegen, and A. Natsch, A functional ABCC11 allele is essential in the biochemical formation of human axillary odor, J. Invest. Dermatol., 130, 529 (2010). https://doi.org/10.1038/jid.2009.254
  10. A. L. Cogen, K. Yamasaki, K. M. Sanchez, R. A. Dorschner, Y. Lai, D. T. MacLeod, J. W. Torpey, M. Otto, V. Nizet, J. E. Kim, and R. L. Gallo, Selective antimicrobial action is provided by phenol-soluble modulins derived from Staphylococcus epidermidis, a normal resident of the skin, J. Invest. Dermatol., 130, 192 (2010). https://doi.org/10.1038/jid.2009.243
  11. I. Wanke, H. Steffen, C. Christ, B. Krismer, F. Gotz, A. Peschel, M. Schaller, and B. Schittek, Skin commensals amplify the innate immune response to pathogens by activation of distinct signaling pathways, J. Invest. Dermatol., 131, 382 (2011). https://doi.org/10.1038/jid.2010.328
  12. S. Parvez, K. A. Malik, S. A. Kang, and H. Y. Kim, Probiotics and their fermented food products are beneficial for health, J. Appl. Microbiol., 100, 1171 (2006). https://doi.org/10.1111/j.1365-2672.2006.02963.x
  13. P. M. Elias, The skin barrier as an innate immune element, Seminars in Immunopathology, 29, 3 (2007). https://doi.org/10.1007/s00281-007-0060-9
  14. D. N. Fredricks, Microbial ecology of human skin in health and disease, J. Investig. Dermatol. Symp. Proc., 6, 167 (2001). https://doi.org/10.1046/j.0022-202x.2001.00039.x
  15. T. Iwase, Y. Uehara, H. Shinji, A. Tajima, H. Seo, K. Takada, T. Agata, and Y. Mizunoe, Staphylococcus epidermidis esp inhibits Staphylococcus aureus biofilm formation and nasal colonization, Nature, 465, 346 (2010). https://doi.org/10.1038/nature09074
  16. M. Kimura, Preponderance of synonymous changes as evidence for the neutral theory of molecular evolution, Nature, 267, 275 (1977). https://doi.org/10.1038/267275a0
  17. S. Kumar, K. Tamura, and M. Nei, MEGA3: Integrated software for molecular evolutionary genetics analysis and sequence alignment, Brief. Bioinform., 5, 150 (2004). https://doi.org/10.1093/bib/5.2.150
  18. S. V. Dhotre, G. T. Mehetre, M. S. Dharne, N. M. Suryawanshi, and B. S. Nagoba, Isolation of Streptococcus tigurinus - a novel member of Streptococcus mitis group from a case of periodontitis. FEMS Microbiol Lett. 357, 131 (2014).
  19. E. B. Brandt and U. Sivaprasad, Th2 Cytokines and Atopic Dermatitis, J. Clin. Cell Immunol., 2, 110 (2011).
  20. S. R. Wilson, L. Thé, L. M. Batia, K. Beattie, G. E. Katibah, S. P. McClain, M. Pellegrino, D. M. Estandian, and D. M. Bautista, The epithelial cell-derived atopic dermatitis cytokine TSLP activates neurons to induce itch, Cell, 155, 285 (2013). https://doi.org/10.1016/j.cell.2013.08.057
  21. H. M. Hamer, D. M. A. E. Jonkers, K. Venema, S. A. L. W. Vanhoutvin, F. J. Troost, and R. J. Brummer, Review article: the role of butyrate on colonic function. AP&T, 27, 104 (2008).
  22. S. H. Kaufmann, The contribution of immunology to the rational design of novel antibacterial vaccines. Nature Reviews Microbiology, 5, 491 (2007). https://doi.org/10.1038/nrmicro1688
  23. K. H. Hänel, C. Cornelissen, B. Lüscher, and J. M. Baron, Cytokines and the Skin Barrier, Int. J. Mol. Sci., 14, 6720 (2013). https://doi.org/10.3390/ijms14046720