Korean Journal of Ichthyology (한국어류학회지)

The Ichthyological Society of Korea (한국어류학회)
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Genetic Species Identification by Sequencing Analysis of Nuclear and Mitochondrial Genes for Albino Misgurnus Species from Korea

우리나라 미꾸리속(genus Misgurnus) 알비노 개체의 미토콘드리아 및 핵 유전자 염기서열 분석에 의한 유전적 동정

  • Song, Ha-Youn (Inland Fisheries Research Institute, National Institute of Fisheries Science) ;
  • Moon, Shin-Joo (Life Science and Biotechnology, Soonchunhyang University) ;
  • Kim, Keun-Sik (East Sea Research Institute, Korea Institute of Ocean Science and Technology) ;
  • Bang, In-Chul (Life Science and Biotechnology, Soonchunhyang University)
  • 송하윤 (국립수산과학원 중앙내수면연구소) ;
  • 문신주 (순천향대학교 생명시스템학과) ;
  • 김근식 (한국해양과학기술원 동해연구소) ;
  • 방인철 (순천향대학교 생명시스템학과)
  • Received : 2017.06.08
  • Accepted : 2017.06.28
  • Published : 2017.06.30
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Abstract

The spontaneous color mutant, albino individuals of genus Misgurnus, are rarely discovered in Korea and there are difficult to identify morphological species due to lack melanin pigmentation. In this study, we developed a genetic identification method for the species of albino Misgurnus individuals based on phylogenetic analysis by using recombination activating gene 1 (rag1) and cytochrome b (cytb) region of mitochondrial DNA. As a result of molecular phylogenetic analysis, three clades were identified as Misgurnus mizolepis, M. anguillicaudatus and M. mohoity. The homology of the cytb sequences of M. mohoity was best match to that of M. mohoity sequences in GenBank database. As a result of species identification of 25 albino Misgurnus individuals based on the phylogenetic tree, the red-eye type was identified as 16 M. anguillicaudatus and one M. mizolepis. The remaining three individuals were identified as one M. mizolepis ♀${\times}$M. anguillicaudatus ♂, and two M. mohoity ♀${\times}$M. anguillicaudatus ♂, respectively. In addition, the five black-eye type individuals were identified as one M. anguillicaudatus, three M. mizolepis and one M. mohoity. Therefore, this genetic identification method will be an useful techniques for species or hybrid identification in genus Misgurnus.

최근 우리나라에서 자연발생적인 미꾸리속 알비노 개체들이 낮은 빈도로 출현하고 있으나, 색소 결핍으로 인해 형태적종 동정이 어렵다. 따라서 본 연구에서는 핵 유전자인 recombination activating gene 1 (rag1) 영역 및 미토콘드리아 유전자 cytochrome b (cytb) 영역을 이용한 분자계통학적 분석을 이용해 미꾸리속 알비노 개체의 분자동정을 수행하였다. 그 결과 rag1과 cytb의 분자계통도에서 미꾸라지, 미꾸리, 그리고 M. mohoity로 3개의 clade가 확인되었다. 확보된 M. mohoity의 염기서열을 유전자은행의 BLAST를 이용해 유사성을 검색한 결과 M. mohoity와 가장 유사하였다. 분자계통도를 기준으로 25마리의 알비노 미꾸리속 개체의 종 동정을 수행한 결과 빨간 눈 타입은 미꾸리 16마리, 미꾸라지 1마리로 판별되었고, 나머지 3개체는 미꾸라지♀${\times}$미꾸리♂ 잡종 1마리와 M. mohoity ♀${\times}$미꾸리♂ 잡종이 2마리 판별되었다. 또한 검은 눈타입 5마리는 미꾸리 1마리와 미꾸라지 3마리 및 M. mohoity 1마리로 판별되었다. 따라서 본 연구에 이용한 분자마커를 활용함으로써 미꾸리속 어류의 정확한 종 또는 잡종을 동정하기 위한 유용한 방법으로 이용될 수 있을 것이다.

Keywords

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