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Isoprenylated flavonoids from the root bark of Morus alba L. and their inhibition effect on NO production in LPS-induced RAW 264.7 cells

  • Jung, Jae-Woo (Graduate School of Biotechnology and Department of Oriental Medicine Biotechnology, Kyung Hee University) ;
  • Ko, Jung-Hwan (Graduate School of Biotechnology and Department of Oriental Medicine Biotechnology, Kyung Hee University) ;
  • Ko, Won-Min (Institute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University) ;
  • Park, Ji-Hae (Graduate School of Biotechnology and Department of Oriental Medicine Biotechnology, Kyung Hee University) ;
  • Baek, Yun-Su (Floriculture Research Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Kim, Youn-Chul (Institute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University) ;
  • Baek, Nam-In (Graduate School of Biotechnology and Department of Oriental Medicine Biotechnology, Kyung Hee University)
  • Received : 2017.02.14
  • Accepted : 2017.03.05
  • Published : 2017.06.30

Abstract

The root bark of Morus alba L. were extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH, and $H_2O$ fractions. The repeated silica gel ($SiO_2$), octadecyl $SiO_2$ (ODS), and Sephadex LH-20 column chromatographies of the EtOAc fraction led to isolation of 12 phenolic compounds. The chemical structures of the compounds were determined as sanggenol Q (1), sanggenol A (2), sanggenol L (3), kuwanon T (4), cyclomorusin (5), sanggenon F (6), sanggenol O (7), sanggenon N (8), sanggenon G (9), mulberrofuran G (10), mulberrofuran C (11), and moracin E (12). All isolated compounds were evaluated for inhibit lipopolysaccharide-induced nitric oxide production in RAW 264.7 macrophages.

Keywords

References

  1. Ahn DK (2012) Sinnongbonchogyung. Euiseungdang, Seoul
  2. Chun JM, Cheon MS, Park M, Lee AY, Moon BC, Ji Y, Kim HK (2012) Inhibitory effects of an ethyl acetate fraction from Cephalonoplos segetum on inflammatory mediators from lipopolysaccharide-induced RAW 264.7 macrophages. J Korean Soc Appl Biol Chem 55: 41-46
  3. Jung JW, Ko WM, Park JH, Seo KH, Oh EJ, Lee DY, Lee DS, Kim YC, Lim DW, Han D, Baek NI (2015) Isoprenylated flavonoids from the root bark of Morus alba and their hepatoprotective and neuroprotective activities. Arch Pharm Res 38: 2066-2075 https://doi.org/10.1007/s12272-015-0613-8
  4. Lee SH, Seo GS, Sohn DH (2004) Inhibition of lipopolysaccharide-induced expression of inducible nitric oxide synthase by butein in RAW 264.7 cells. Biochem Biophys Res Commun 323: 125-132 https://doi.org/10.1016/j.bbrc.2004.08.063
  5. Naoki A, Toru Y, Kayo Y, Kyoto I, Haruhisa K, Yukihiko K, Atsushi K, Robert JN, Lee HS, Ryu KS (2001) Polyhydroxylated alkaloids isolated from mulberry trees (Morus alba L.) and silkworms (Bombyx mori L.). J Agric Food Chem 49: 4208-4213 https://doi.org/10.1021/jf010567e
  6. Yang ZG, Matsuzaki K, Takamatsu S, Kitanaka (2011) Inhibitory effects of constituents from Morus alba var. multicaulis on differentiation of 3T3-L1 cells and nitric oxide production in RAW264.7 cells. Molecules 16: 6010-6022 https://doi.org/10.3390/molecules16076010

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