Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Circulating Plasma and Exosomal microRNAs as Indicators of Drug-Induced Organ Injury in Rodent Models

  • Cho, Young-Eun (Department of Molecular Medicine, CMRI, School of Medicine, Kyungpook National University) ;
  • Kim, Sang-Hyun (Department of Pharmacology, CMRI, School of Medicine, Kyungpook National University) ;
  • Lee, Byung-Heon (Department of Biochemistry and Cell Biology, CMRI, School of Medicine, Kyungpook National University) ;
  • Baek, Moon-Chang (Department of Molecular Medicine, CMRI, School of Medicine, Kyungpook National University)
  • Received : 2016.08.04
  • Accepted : 2016.12.13
  • Published : 2017.07.01
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Abstract

This study was performed to evaluate whether microRNAs (miRNAs) in circulating exosomes may serve as biomarkers of drug-induced liver, kidney, or muscle-injury. Quantitative PCR analyses were performed to measure the amounts of liver-specific miRNAs (miR-122, miR-192, and miR-155), kidney-specific miR-146a, or muscle-specific miR-206 in plasma and exosomes from mice treated with liver, kidney or muscle toxicants. The levels of liver-specific miRNAs in circulating plasma and exosomes were elevated in acetaminophen-induced liver injury and returned to basal levels by treatment with antioxidant N-acetyl-cysteine. Circulating miR-146a and miR-206 were increased in cisplatin-induced nephrotoxicity and bupivacaine-induced myotoxicity, respectively. Taken together, these results indicate that circulating plasma and exosomal miRNAs can be used as potential biomarkers specific for drug-induced liver, kidney or muscle injury.

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References

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