분석과학 (Analytical Science and Technology)

  • 제30권3호
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  • Pages.146-153
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  • 2017
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  • 1225-0163(pISSN)
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  • 2288-8985(eISSN)
한국분석과학회 (The Korean Society of Analytical Sciences)
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Ginsenosides analysis in the crude saponin fraction extracted from Korean red ginseng, and its efficacious analysis against acute pulmonary inflammation in mice

  • Lee, Seung Min (Department of Industrial Plant Science & Technology, Chungbuk National University) ;
  • Lim, Heung Bin (Department of Industrial Plant Science & Technology, Chungbuk National University)
  • 투고 : 2017.03.20
  • 심사 : 2017.05.08
  • 발행 : 2017.06.25
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초록

In this study, we isolated ginseng crude saponin (GCS) from Korean red ginseng (KRG) and determined the ginsenoside content in it to investigate the physiological and pathological effects of GCS on acute pulmonary inflammation induced by intratracheal instillation of cigarette smoke condensates (CSC) and lipopolysaccharide (LPS) solution in BALB/c mice. GCS was orally administered at doses of 10 mg/kg and 25 mg/kg for 3 weeks. The recovery rate of GCS from KRG was 6.5 % and total ginsenosides from GCS was 1.13 %, and the content of Rb1 was the highest among them. Total inflammatory cells in the lung homogenates and bronchoalveolar lavage fluid (BALF) increased following intratracheal administration of CSC and LPS. However, GCS administration impaired this increase. Furthermore, it inhibited the increase in leukocytes in the blood, considerably decreased neutrophils in BALF, and declined infiltration of inflammatory cells and deposition of collagen in the tracheal and alveolar tissue. In this study, GCS was found to have a protective effect against acute pulmonary inflammation and it may be beneficial in preventing various respiratory diseases.

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