한국발생생물학회지:발생과생식 (Development and Reproduction)

  • 제21권1호
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  • Pages.35-46
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  • 2017
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  • 2465-9525(pISSN)
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  • 2465-9541(eISSN)
한국발생생물학회 (The Korean Society of Developmental Biology)
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Serum Cytokine Levels are related to Nesfatin-1/NUCB2 Expression in the Implantation Sites of Spontaneous Abortion Model of CBA/j×DBA/2 Mice

  • Chung, Yiwa (Department of Bioenvironmental Technology, College of Natural Sciences, Seoul Women's University) ;
  • Kim, Heejeong (Department of Bioenvironmental Technology, College of Natural Sciences, Seoul Women's University) ;
  • Seon, Sojeong (Department of Bioenvironmental Technology, College of Natural Sciences, Seoul Women's University) ;
  • Yang, Hyunwon (Department of Bioenvironmental Technology, College of Natural Sciences, Seoul Women's University)
  • 투고 : 2017.02.02
  • 심사 : 2017.02.14
  • 발행 : 2017.03.31
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초록

The process of spontaneous abortion involves a complex mechanism with various cytokines, growth factors, and hormones during the pregnancy. However, the mechanism underlying spontaneous abortion by pro- and anti-inflammatory cytokines in the serum during the pregnancy is not fully understood. Therefore, the purpose of this study was to examine the relationship between the serum levels of pro- and anti-inflammatory cytokines and spontaneous abortion using the $CBA/j{\times}DBA/2$ mouse model. Serum levels of pro-inflammatory cytokines, such as $IFN-{\gamma}$, $IL-1{\alpha}$ and $TNF-{\alpha}$ were not increased in abortion model mice, but anti-inflammatory cytokines, such as IL-4, IL-13 and IL-1ra were decreased compared to normal pregnant mice. In addition, serum levels of chemokine, such as SDF-1, G-CSF, M-CSF, IL-16, KC and MCP-1 were decreased in abortion model mice compared to normal pregnant mice. However, the expression levels of nesfatin-1/NUCB2 mRNA and protein in the uteri of implantation sites were significantly higher in abortion model mice than normal pregnant mice. These results suggest that uterine nesfatin-1/NUCB2 expression may be down-regulated by inflammatory cytokines and chemokines in the serum of pregnant mice. Moreover, this study suggests the possibility that nesfatin-1/NUCB2 expressed in the implantation sites may be associated with the maintenance of pregnancy.

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