Korean Journal of Acupuncture

Society for Meridian and Acupoint (경락경혈학회)
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The Effect of Lactobacillus Mixture Culture Fluid Extracts on Atopic Dermatitis Chemokine Expression of in HaCaT Cells

HaCaT 세포에서 Lactobacillus 혼합배양액 추출물이 아토피관련 케모카인 발현에 미치는 효과

  • Hong, Soo-Jeong (Department of Health Care Management Administration, Graduate School of Business Administration, Gachon University) ;
  • Lee, Won-Jae (Department of Health Care Management Administration, Graduate School of Business Administration, Gachon University) ;
  • Jo, Eul-Hwa (Department of Meridians and Acupoints, College of Korean Medicine, Wonkwang University) ;
  • Ahn, Seong-Hun (Department of Meridians and Acupoints, College of Korean Medicine, Wonkwang University)
  • 홍수정 (가천대학교 경영대학원 의료경영학교실) ;
  • 이원재 (가천대학교 경영대학원 의료경영학교실) ;
  • 조을화 (원광대학교 한의과대학 경혈학교실) ;
  • 안성훈 (원광대학교 한의과대학 경혈학교실)
  • Received : 2017.03.28
  • Accepted : 2017.05.31
  • Published : 2017.06.27
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Abstract

Objectives : Recently the case of lactobacillus mixture culture fluid appliment was reported. In this study, anti-inflamation effects and anti-allergy effects were studied by stimulus of lactobacillus mixture culture fluid extracts in HaCaT cells. Methods : The atopic dermatitis were induced by TNF-${\alpha}$ and interferon-${\gamma}$ in HaCaT cells. TARC/CCL17, MDC/CCL22, RANTES/CCL5 and ROS production were investigated to explain anti-inflamation and allergy effects of lactobacillus mixture culture fluid with cell-enzyme-linked Immunosorbent assay in 450 nm, 485 nm, 535 nm with spectro-fluorometer. Results : The extracts of lactobacillus mixture culture fluid were decreased TARC/CCL17, MDC/CCL22, RANTES/CCL5 expressions and ROS production with a concentration dependent manner. Conclusions : The effects mechanism of Lactobacillus mixed culture fluid for atopic dermatitis symptoms were considered to be explain anti-inflamation and allergy effects via control of cytokine, chemokine and ROS production, and the fluid could be applied in skin cells directly. But classified AD symptom degrees reported in clinical case before as Reaction Period, Reduction Period, Effect Period, Reproduction Period and Rebound Period could not be explained. Further study will be expected.

Keywords

References

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