Journal of Plant Biotechnology

  • 제44권1호
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  • Pages.97-106
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  • 2017
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  • 1229-2818(pISSN)
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  • 2384-1397(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
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신규 수입 승인 6개 유전자변형작물의 검출기법 개발

Development of detection methods for six approved LM crops in Korea

  • 설민아 (국립생태원 생태연구본부 생태보전연구실) ;
  • 조범호 (충남테크노파크 바이오센터 생물산업팀) ;
  • 최원균 (국립생태원 생태연구본부 생태보전연구실) ;
  • 신수영 (국립생태원 생태연구본부 생태보전연구실) ;
  • 엄순재 (국립생태원 생태연구본부 생태보전연구실) ;
  • 김일룡 (국립생태원 생태연구본부 생태보전연구실) ;
  • 송해룡 (국립생태원 생태연구본부 생태보전연구실) ;
  • 이중로 (국립생태원 생태연구본부 생태보전연구실)
  • Seol, Min-A (Division of Ecological Conservation, National Institute of Ecology (NIE)) ;
  • Jo, Beom-Ho (Bio-industrial Team, Bio Center, Chungnam Techno Park) ;
  • Choi, Wonkyun (Division of Ecological Conservation, National Institute of Ecology (NIE)) ;
  • Shin, Su Young (Division of Ecological Conservation, National Institute of Ecology (NIE)) ;
  • Eum, Soon-Jae (Division of Ecological Conservation, National Institute of Ecology (NIE)) ;
  • Kim, Il Ryong (Division of Ecological Conservation, National Institute of Ecology (NIE)) ;
  • Song, Hae-Ryong (Division of Ecological Conservation, National Institute of Ecology (NIE)) ;
  • Lee, Jung Ro (Division of Ecological Conservation, National Institute of Ecology (NIE))
  • 투고 : 2017.01.05
  • 심사 : 2017.02.08
  • 발행 : 2017.03.31
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초록

일반적으로 유전자를 재조합하는 생명공학기술이 널리 이용되고 있는 것은 유전자변형 작물 분야이다. LM 제품에 대한 의존도가 높아짐에 따라 한국에서는 LMO의 안전성에 대한 우려가 지속적으로 증가하고 있다. 따라서, 우리는 자연환경 내 비의도적으로 방출된 LMO를 판별할 수 있는 검출기법을 확립하였다. JRC 정보를 토대로 6개 LM 이벤트(캐놀라 1, 옥수수 1, 콩 4개)의 유전자를 검출할 수 있는 PCR 조건을 확립하였다. 각 LM 시료에서 genomic DNA를 분리하였고, 이벤트 특이적인 프라이머를 사용하여 PCR 실험을 수행하였다. 또한 자체적으로 확립된 검출법에 의해 모든 LMO의 이벤트 특이적 유전자가 검출되었다. LM 유전자의 삽입 위치를 분석하기 위해 PCR 생성물을 이용하여 염기서열을 분석하였다. 이 결과는 LM 이벤트의 특이적인 유전자를 효율적으로 검출할 수 있음을 나타낸다. 게다가 본 연구결과 확립된 검출기법은 자연환경 내 LM 작물의 모니터링 및 사후 관리에 활용될 것이다.

Living modified crops are genetically modified living organisms and are widely used in biotechnical research and desired goods. As the reliance on LM products, concerns about safety of LMOs have been continuously increased in South Korea. We established the detection methods for unintentional released LMOs in environmental conditions. To detect six LM event genes of 1 canola, 1 maize and 4 soybeans, PCR conditions were based upon consideration of the Joint Research Centre information. Genomic DNAs were isolated from LM samples and PCR analysis were performed using each event-specific primer pair. Event-specific genes of all events were efficiently recognized by our methods. To investigate the insertion site of LM genes in each genome, we verified PCR product sequence by DNA sequencing. These results suggest that the LM event-specific gene amplification can be efficiently developed. In addition, our detection method is fit for monitoring and post-management of LM crops in the environment.

키워드

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