Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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In vitro anti-inflammatory activity of extracts from Potentilla supina in murine macrophage RAW 264.7 cells

개소시랑개비 추출물의 RAW264.7대식세포에서 in vitro 항염효과

  • Nam, Jung-Hwan (Highland Agriculture Research Institute, National Institute of Crop Science, RDA) ;
  • Kim, Hyun-Sam (Agricultural support, Yanggu County Office) ;
  • Kim, Byoumg-Jin (Agricultural Technology Center, Yanggu County Office) ;
  • Yu, Hong-Seob (Highland Agriculture Research Institute, National Institute of Crop Science, RDA) ;
  • Chang, Dong-Chil (Highland Agriculture Research Institute, National Institute of Crop Science, RDA) ;
  • Jin, Yong-Ik (Highland Agriculture Research Institute, National Institute of Crop Science, RDA) ;
  • Yoo, Dong-Lim (Highland Agriculture Research Institute, National Institute of Crop Science, RDA) ;
  • Choi, Jong-Keun (Highland Agriculture Research Institute, National Institute of Crop Science, RDA) ;
  • Park, Hee-Jhun (Dept. of Pharmaceutical Engineering, College of Health Sciences, Sang-ji University) ;
  • Lee, Seung-Bin (Dept. of pharmaceutical, College of Pharmacy, Kyung-Hee University) ;
  • Lee, Kyung-Tea (Dept. of pharmaceutical, College of Pharmacy, Kyung-Hee University) ;
  • Park, Soo-Jin (Dept. of Food and Nutrition, Semyung University)
  • 남정환 (농촌진흥청, 국립식량과학원, 고령지농업연구소) ;
  • 김현삼 (양구군청, 농업지원과) ;
  • 김병진 (양구군청, 농업기술센터) ;
  • 유홍섭 (농촌진흥청, 국립식량과학원, 고령지농업연구소) ;
  • 장동칠 (농촌진흥청, 국립식량과학원, 고령지농업연구소) ;
  • 진용익 (농촌진흥청, 국립식량과학원, 고령지농업연구소) ;
  • 유동림 (농촌진흥청, 국립식량과학원, 고령지농업연구소) ;
  • 최종근 (농촌진흥청, 국립식량과학원, 고령지농업연구소) ;
  • 박희준 (상지대학교, 보건대학, 제약공학과) ;
  • 이승빈 (경희대학교, 약학대학, 약학과) ;
  • 이경태 (경희대학교, 약학대학, 약학과) ;
  • 박수진 (세명대학교, 식품영양학과)
  • Received : 2016.11.25
  • Accepted : 2017.01.27
  • Published : 2017.03.31
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Abstract

Potentilla supina (Rosaceae) has traditionally been used to treat disorders of hemostasis, dysentery, malaria, bloody discharge and arthritis, and it has antinociceptive and anti-inflammatory properties. However, validity of the anti-inflammatory activity has not been scientifically investigated so far. Therefore, the aim of this study was to investigate the anti-inflammatory potential of P. supina using the ethanolic extract of P. supina and its sub-fractions. To evaluate the anti-inflammatory effects of P. supina, we examined the inflammatory mediators such as nitric oxide (NO), inducible nitric oxide synthase (iNOS) and prostaglandin $E_2$ ($PGE_2$) in RAW 264.7 cells. Our results indicated that ethyl acetate fraction significantly inhibited LPS-induced NO, iNOS and $PGE_2$ production in RAW 264.7 cells. This result showed that ethyl acetate fraction of P. supina is expected to be a good candidate for development into a source of anti-inflammatory agents.

본 연구에서는 개소시랑개비 (Potentilla supina)의 전초를 이용하여 세포독성 및 항염증 활성 효과를 평가하였다. 대식세포인 RAW264.7 cell에서 염증 매개 물질인 lipopo-lysacchride(LPS)로 염증을 유발해 nitric oxide (NO), Inducible nitric oxide synthase (iNOS)와 prostaglandin$E_2$($PGE_2$) 같은 염증 유발 인자들의 억제효과를 확인하였다. 개소시랑개비 ethyl acetate 분획물의 염증 유발 인자 억제 시 $IC_{80}$ value를 측정하였을 때 nitric oxide 및 prostaglandin $E_2$ 생성을 농도의존적으로 현저하게 저해하는 농도는 각각 29.34와 $50.75{\mu}g/ml$이었고 Inducible nitric oxide synthase의 양도 $50{\mu}g/ml$ 처리하였을때 농도 의존적으로 발현 감소 하였다. 따라서 본 연구결과는 개소시랑개비의 ethyl acetate 분획물과 같은 비극성용매 분획물들이 유의성 있는 항염증 효과를 나타내었으며, 이러한 효능은 예방의학적 가능성을 충분히 가지고 있기에 염증성질환의 예방을 위한 건강 기능성식품의 개발 가능성을 제시할 수 있을 것으로 기대된다. 또한 염증과 관련된 사이토카인 및 단백질 발현 메커니즘에 대한 추가적인 연구가 필요할 것으로 판단된다.

Keywords

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