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전라북도에서 곤충 병원성 곰팡이 Zoophthora radicans (Entomophthorales: Entomophthoraceae)의 발생

Occurrence of the Entomopathogenic Fungus Zoophthora radicans (Entomophthorales: Entomophthoraceae) in Jeollabuk-do, Korea

  • Choi, Seonu (Jeollabuk-do Agricultural Research and Extension Services) ;
  • Kim, Juhee (Jeollabuk-do Agricultural Research and Extension Services) ;
  • Seo, Kyoungwon (Jeollabuk-do Agricultural Research and Extension Services) ;
  • Moon, Younghun (Jeollabuk-do Agricultural Research and Extension Services) ;
  • Lee, Gongjun (Jeollabuk-do Agricultural Research and Extension Services) ;
  • Lee, Changkyu (Jeollabuk-do Agricultural Research and Extension Services) ;
  • Kim, Jaesu (Department of Agricultural Biology, College of Agriculture & Life Scinece, Chonbuk National University) ;
  • Kim, Jinho (Jeollabuk-do Agricultural Research and Extension Services)
  • 투고 : 2016.11.04
  • 심사 : 2017.05.17
  • 발행 : 2017.06.01

초록

곤충병원성 곰팡이 Zoophthora radicans (Entomophthorales: Entomophthoraceae)가 혹명나방, 배추좀나방, 복숭아혹진딧물과 진딧물인 미분류 1종을 감염시키는 것을 발견하였다. 이들은 전라북도 진안, 김제, 익산에서 수집되었고, 6월과 9월에 관찰이 가능하였다. 이 균에 감염된 혹명나방은 진안에서 수집되었고, 감염율이 46%에 달하였다. 감염된 곤충 충체로부터 균을 분리하여 Sabouraud dextrose agar with yeast extract (SDAY)를 이용하여 인공배양을 시도하였다. 그 결과, $20{\sim}28^{\circ}C$가 인공배양을 위한 최적온도로 판단되며, SDAY보다 SDAY supplemented with egg yolk and milk (SDAY-EM)에서 발육이 보다 양호하였다. 이 곰팡이는 자연적으로 발생함을 포장에서 확인할 수 있었다.

The entomopathogenic fungus Zoophthora radicans (Entomophthorales: Entomophthoraceae) was found to infect Cnaphalocrocis medinalis, Plutella xylostella, Myzus persicae and one unidentifed species. Samples of the fungus were collected from three areas (Jinan, Gimje, and Iksan) in Jeollabuk-do, Korea. Infected insects appeared in July and September, during which time population infection rate of Z. radicans on C. medinalis reached up to 46%. Zoophthora radicans hyphal bodies are rod-like to hyphoid, and primary conidia are bullet-shaped to long ovoid. The fungus was isolated from the surfaces of larvae and cultured in Sabouraud dextrose agar with yeast extract (SDAY). The optimal temperature range for mycelial growth was $20{\sim}28^{\circ}C$. Mycelium growth on SDAY supplemented with egg yolk and milk (SDAY-EM) was higher than on SDAY alone. Zoophthora radicans may play an important role in controlling insect population density using naturally induced epizootics.

키워드

참고문헌

  1. Alves, L.F., Leite, L.G., de Oliveira, D.G., 2009. First record of Zoophthora radicans (Entomophthorales: Entomophthoraceae) on adults of the paraguay tea Psyllid, Gyropsylla spegazziniana Lizer & Trelles (Hemiptera: Psyllidae), in Brazil. Neotropical entomology 38, 697-698. https://doi.org/10.1590/S1519-566X2009000500025
  2. Brefeld, O., 1870. Entwicklungsgeschichte der Empusa muscae und Empusa radicans. Botanische Zeitschrift, 6.
  3. Choi, S.W., Lim, J.R., Moon, H.C., Kim, J.H., Song, Y.J., Nai, Y.S., Kim, J.S., 2016. Aedia leucomelas (Lepidoptera: Noctuidae) - pathogenic Entomophaga aulicae (Zygomycetes: Entomophthorales) in sweet potato fields. Journal of Asia-Pacific Entomology 19, 71-79. https://doi.org/10.1016/j.aspen.2015.11.008
  4. Furlong, M.J., Pell, J.K., 1997. The influence of environmental factors on the persistence of Zoophthora radicans conidia. Journal of Invertebrate Pathology 69, 223-233. https://doi.org/10.1006/jipa.1996.4649
  5. Furlong, M.J., Pell, J.K., Choo, O.P., Rahman, S.A., 1995. Field and laboratory evaluation of a sex pheromone trap for the autodissemination of the fungal entomopathogen Zoophthora radicans (Entomophthorales) by the diamondback moth, Plutella xylostella (Lepidoptera: Yponomeutidae). Bulletin of Entomological research 85, 331-337. https://doi.org/10.1017/S0007485300036051
  6. Galaini-Wraight, S., Wraight, S.P., Carruthers, R.I., Magalhaes, B.P., Roberts, D., 1991. Description of a Zoophthora radicans (Zygomycetes: Entomophthoraceae) epizootic in a population of Empoasca kraemeri (Homoptera: Cicadellidae) on beans in central Brazil. Journal of Invertebrate Pathology 58, 311-326. https://doi.org/10.1016/0022-2011(91)90176-Q
  7. Glare, T.R., Milner, R.J., Chilvers, G.A., 1986. The effect of environmental factors on the production, discharge, and germination of primary conidia of Zoophthora phalloides Batko. Journal of Invertebrate Pathology 48, 275-283. https://doi.org/10.1016/0022-2011(86)90055-8
  8. Glare, T.R.A.R.J.M., 1991. Ecology of entomopathogenic fungi. In Arora, D. K., L. Ajello, and K. G. Mukerji. (Eds.), Handbook of applied mycology. 2. Humans, animals, and Insects. Marcel Dekker, New York, 547-612.
  9. Griggs, M., Vandenberg, J., Sawyer, A., 1999. Effect of relative humidity on viability of primary conidia of Zoophthora radicans. Journal of invertebrate pathology 73, 315-320. https://doi.org/10.1006/jipa.1999.4849
  10. Hodge, K.T., Sawyer, A.J., Humber, R.A., 1995. RAPD-PCR for identification of Zoophthora radicans isolates in biological control of the potato leafhopper. Journal of invertebrate pathology 65, 1-9. https://doi.org/10.1006/jipa.1995.1001
  11. Humber, R.A., 1989. Synopsis of a revised classification for the Entomophthorales (Zygomycotina). Mycotaxon 34, 441-460.
  12. Humber, R.A., 1998. Entomopathogenic fungal identification. APS/ ESA workshop. Cornell. Univ., pp26.
  13. Keller, S., 1987. Arthropod-pathogenic Entomophthorales of Switzerland. I. Conidiobolus, Entomophaga and Entomophthora. Sydowia 40, 122-167.
  14. Khan, Z., Abenes, M., Fernandez, N., 1996. Suitability of graminaceous weed species as host plants for rice leaffolders, Cnaphalocrocis medinalis and Marasmia patnalis. Crop Protection 15, 121-127. https://doi.org/10.1016/0261-2194(95)00066-6
  15. Latge, J.P., 1981. Comparaison des exigences nutritionnelles des Entomophthorales. Ann. Microbiol. 26, 1038-1048.
  16. Lingappa, S., Basavanagoud, K., Kulkarni, K., Patil, R.S., Kambrekar, D., 2004. Threat to vegetable production by diamondback moth and its management strategies, Fruit and vegetable diseases. Springer, pp. 357-396.
  17. Mazzoglio, P.J., Dolci, P., Lessio, F., Alma, A., 2009. Zoophthora radicans affecting Zyginidia pullula. Bulletin of Insectology 62, 143-148.
  18. Milner, R., Lutton, G., 1983. Effect of temperature on Zoophthora radicans (Brefeld) Batko: an introduced microbial control agent of the spotted alfalfa aphid, Therioaphis trifolii (Monell) f. maculata. Australian Journal of Entomology 22, 167-173. https://doi.org/10.1111/j.1440-6055.1983.tb01865.x
  19. Mota-Sanchez, D., Bills, P.S., Whalon, M.E., 2002. Arthropod resistance to pesticides: status and overview. Pesticides in Agriculture and the Environment, 241-272.
  20. Park, H.H., Cho, J.R., Park, C.G., Kim, K.H., Goh, H.G., Lee, S.G., 2010. The Occurrence of Rice Leaf-folder, Cnaphalocrocis medinalis (Lepidoptera: Crambidae) in Suwon and its Responses to Insecticides. Korean journal of applied entomology 49, 219-226. https://doi.org/10.5656/KSAE.2010.49.3.219
  21. Pell, J., Eilenberg, J., Hajek, A., Steinkraus, D., 2001. Biology, ecology and pest management potential of Entomophthorales. Fungi as biocontrol agents: progress, problems and potential. CAB International, Wallingford, 71-153.
  22. Remaudiere, G., Hennebert, G., 1980. Revision systematique de Entomophthora aphidis Hoffm. in Fres. Description de deux nouveaux pathogenes d'aphides. Mycotaxon.
  23. Riethmacher, G., Rombach, M., Kranz, J., 1992. Epizootics of Pandora blunckii and Zoophthora radicans (Entomophthoraceae: Zygomycotina) in diamondback moth populations in the Philippines, Diamondback moth and other crucifer pests: proceedings of the Second International Workshop, Tainan, Taiwan, 10-14 December 1990/NS Talekar, editor. Taipei: Asian Vegetable Research and Development Center, c1992.
  24. Sanchez-Pena, S.R., 2000. Infectivity of Zoophthora radicans (Zygomycetes: Entomophthorales) Towards Trialeurodes vaporariorum (Homoptera: Aleyrodidae) Nymphs. The Florida Entomologist 83, 101-105. https://doi.org/10.2307/3496236
  25. Talekar, N., Shelton, A., 1993. Biology, ecology, and management of the diamondback moth. Annual review of entomology 38, 275-301. https://doi.org/10.1146/annurev.en.38.010193.001423
  26. Team, R.C., 2014. R: A Language and Environment for Statistical Computing. Vienna: R Foundation for Statistical Computing. Available online at: http. www. R-project.org.
  27. Thomas, M.B., Blanford, S., 2003. Thermal biology in insect-parasite interactions. Trends in Ecology & Evolution 18, 344-350. https://doi.org/10.1016/S0169-5347(03)00069-7
  28. Velasco-Silva, J., Alatorre-Rosas, R., Pell, J., Guzman-Franco, A., 2000. Characterization of native entomophthoralean fungi associated with Plutella xylostella (Lepidoptera: Plutellidae) in the Bajio region, Guanajuato, Mexico, 33rd annual meeting of the society for invertebrate pathology. University of Guanajuato, Mexico.
  29. Wilding, N., 1971. Discharge of conidia of Entomophthora thaxteriana Petch from the pea aphid Acyrthosiphon pisum Harris. Microbiology 69, 417-422.
  30. Wolf, F.T., 1951. The cultivation of two species of Entomophthora on synthetic media. Bulletin of the Torrey Botanical Club, 211-220.
  31. Wraight, S., Galaini-Wraight, S., Carruthers, R., Roberts, D., 2003. Zoophthora radicans (Zygomycetes: Entomophthorales) conidia production from naturally infected Empoasca kraemeri and dry-formulated mycelium under laboratory and field conditions. Biological Control 28, 60-77. https://doi.org/10.1016/S1049-9644(03)00035-5
  32. Yoon, C.-S., Yun, T.-Y., Lee, G.-H., Yoo, J.-K., 1998. First record of the entomopathogenic fungus Zoophthora radicans on the green peach aphid Myzus persicae in Korea. The Korean Journal of Mycology 26, 399-402.