Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
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Effect of Acclimation Methods on Physiological Status of White Shrimp, Litopenaeus vannamei Larvae to Low Salinities

흰다리새우 유생의 저염분 순치방법에 따른 생화학적 특성변화

  • 김수경 (국립수산과학원 서해수산연구소) ;
  • 심나영 (국립수산과학원 서해수산연구소) ;
  • 장진우 (국립수산과학원 서해수산연구소) ;
  • 전제천 (국립수산과학원 서해수산연구소) ;
  • 김수경 (국립수산과학원 서해수산연구소) ;
  • 신윤경 (국립수산과학원 남동해수산연구소)
  • Received : 2016.12.02
  • Accepted : 2017.03.09
  • Published : 2017.03.31
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Abstract

This study focused on the physiological change of the shrimp, Litopenaeus vannamei postlarvae 15 stages, under different acclimation methods up to the endpoint of 4 practical salinity unit (psu). Besides using sea water as the control, two acclimation methods, fast acclimation (50% salinity reduction every 8 hours) and slow acclimation (50% salinity reduction every day), were adapted. Results show that the survival rate, glucose and blood uric nitrogen of each group were not significantly different. However, the ion profile differed according to the acclimation methods. Magnesium and sodium of shrimps acclimated to low salinity in both the methods, showed lower concentration than shrimps at 32 psu sea water. Especially, $Na^+$ concentration, which directly influences the osmolality of shrimp, decreased sharply in the fast acclimated group during the first eight hours (from 32 psu to 16 psu). To reduce acclimation stress, it is recommended to take more than eight hours during the first step for reducing the salinity.

본 연구는 흰다리새우 초기유생이 (Postlarvae 15) 4 psu 저염분 순치 시 생리학적 변화에 관한 연구로서 순치방법, 즉 일반 해수를 비교구로 하고 8시간마다 빠르게 염분을 50%씩 감소를 한 실험구 (fast acclimation; F.A.)와 하루에 50%씩 천천히 염분을 감소시킨 실험구 (slow acclimation; S.A.)를 설정하여 생리학적 변화를 비교하였다. 생존율, gloucose와 혈중 요소성 질소의 함량이 순치방법에 따라 유의적인 차이를 보이지 않았으나 이온의 조성은 순치방법에 따라 차이를 보였다. 저염분에 순치된 새우의 체액 조성 중 마그네슘과 나트륨은 염분 32 psu에서 보다 낮은 농도를 보였다. 특히 나트륨 농도는 직접적으로 새우의 삼투압 조절에 영향을 미치는데 빠르게 순치를 시킨 F.A. 실험구에서 급격히 나트륨 농도가 감소하는 것으로 나타났다 (염분 32 psu에서 16 psu로 감소). 순치과정에서 새우의 스트레스를 줄이기 위해서는 처음 50% 염분을 감소시키는 과정은 8시간 이상으로 유지하고 그 이후에는 빠르게 순치하는 방법을 적용하는 것이 바람직할 것으로 파악되었다.

Keywords

References

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