• Title/Summary/Keyword: blood chemistry

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Cell Separation through chemically modified polyurethane membranes

  • Akon Higuchi;Ryoko Hayashi;Yamamiya, Shin-ichi;Hanako Kitamura
    • Proceedings of the Membrane Society of Korea Conference
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    • 2004.05a
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    • pp.55-58
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    • 2004
  • Cell separation from peripheral blood was investigated using surface-modified polyurethane (PU) membranes with different functional groups. Both red blood cells and platelets could pass through unmodified PU and PU-SO$_3$H membranes, while the red blood cells preferentially passed through PU-N(C$_2$H$_{5}$ )$_2$ and PU-NHC$_2$H$_4$OH membranes. The permeation ratio of T and B cells was less than 25% for the surface-modified and unmodified PU membranes. CD34$^{+}$ cells have been recognized as various kinds of stem cells including hematopoietic and mesenchymal stem cells. The adhesiveness of CD34$^{+}$ cells on the PU membranes was found to be higher than that of red blood cells, platelets, T cells or B cells. Overall, the adhesiveness of blood cells on the PU membranes increased in the following order: red blood cells $\leq$ platelets < T cells $\leq$ B cells < CD34$^{+}$ cells. Treatment of PU-COOH membranes with a human albumin solution to detach adhered blood cells, allowed recovery of mainly CD34$^{+}$ cells in the permeate, while both red blood cells and platelets could be isolated in the permeate using unmodified PU membranes. The PU membranes showed different permeation and recovery ratios of specific cells depending on the functional groups attached to the membranes.mbranes.

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Effects of Aloe vera Treatment on Blood Glucose Level and Clinical Chemistry in Diabetic Patients (당뇨병 환자의 혈당 및 임상에 미치는 알로에의 효과)

  • 박웅양;윤여표
    • Journal of Food Hygiene and Safety
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    • v.10 no.1
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    • pp.13-17
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    • 1995
  • In order to study the effects of Aloe vera treatment on blood glucose level and clinical chemistry in diabetic patients, eight diabetic patients were administered orally with 800 mg of Aloe vera three time a day for three months. The high levels of blood and urine glucose in diabetic patients were significantly reduced by administration of Aloe vera. The increased plasma triglyceride concentration was also significantly reduced by Aloe vera treatment. A little amount of urine bilirubin, hematuria, nitrite, urobilinogen, protein and ketone bodies were detected before treatment, but not detected after Aloe vera treatment. But other blood parameters of clinical chemistry values were not affected by Aloe vera treatment. These data suggest that Aloe vera can be effective in the treatment of the diabetic patients.

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Correlation of ketone bodies in blood and spleen

  • Sookyung Jeon;Sumin Lee;Wooyong Park;Chihyun Park;Minjung Kim
    • Analytical Science and Technology
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    • v.36 no.4
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    • pp.170-179
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    • 2023
  • Starvation, diabetes, alcoholism and hypothermia cause ketoacidosis in the human body; therefore, the cause of death can be determined by analyzing ketone bodies in the blood of the deceased. In the case of decomposition of the cadaver, however, since collecting intact blood is impossible, ketone body analysis is performed using the spleen. However, the index for diagnosing ketoacidosis is based on blood concentration, and its correlation with ketone bodies present in the spleen remains unknown. In particular, since decomposition proceeds rapidly during summer, when temperature and humidity are high, understanding the correlation between ketone bodies in the blood and spleen is important to estimate the state at the time of death from a decaying body. Therefore, in the present study, the correlation between ketone bodies in the blood and spleen of the deceased was explored. Ketone bodies (beta-hydroxybutyric acid [BHB] and acetone) in the blood and spleen were analyzed and compared from autopsies (>100 mg·L-1 BHB, blood basis) conducted at the Daejeon Forensic Research Institute from June to December 2021. Moreover, the concentration of ketone bodies in the spleen juice and tissues was compared assuming the scenario of extreme decomposition. Ketone retention concentration in the blood and spleen was positively correlated, and the ratio of BHB concentration in the spleen to BHB concentration in the blood ranged from 0.52 to 1.08 (mean = 0.85 ± 0.12), although the ratio may vary depending on the degree of decomposition of the corpse.

Influence of free stall with concrete floor on profile of blood chemistry and clinico-morphopathogenesis of foot disease in cows (콘크리트우상을 지닌 후리스톨이 젖소의 혈액화학적 성상 및 발굽질환의 임상 형태병리에 미치는 영향)

  • Jeong, Soon-wuk
    • Korean Journal of Veterinary Research
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    • v.35 no.3
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    • pp.625-630
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    • 1995
  • During the period from june 1994 to february 1995 influence of free stall with concrete floor on profile of blood chemistry and clinico-morphopathogenesis of foot disease on 266 cows were studied. The results obtained as follow. 1. No differences between value of blood chemistry in normal and lame cows with foot disease were observed. 2. 20.7% of the cows were clinically lame. 3. Prevalence of clinical digital disorders were investigated: hyperplasia interdigitalis(45.8%), pododermatitis circumscripta(22.4%), dermatitis interdigitalis(9.4%), erosio ungulae(5.9%), phlegmona interdigitalis(3.5%), pododermatitis septica traumatica(3.5%), dermatitis digitalis(2.4%), white line disease(2.4%), pododermatitis aseptica diffusa(2.4%), dermatitis verrucosa(1.2%), fissura ungulae(1.2%) 4. Most claw lesions were located on lateral hindclaws and interdigital space of hindclaws.

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Studies on the Hematologic Values and Blood Chemistry Values of Korean Native Ogolgye II. Blood Chemistry for Korean Native Ogolgye (한국 재래오골계의 혈액상 및 혈액화학치에 관한 연구 - II. 오골계의 혈액화학치)

  • Rhee Han-Gie;Kim Jong-Sub
    • Journal of the korean veterinary medical association
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    • v.20 no.6
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    • pp.371-376
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    • 1984
  • This study was conducted to determine the normal values for chemical components of blood serum were determined or normal 10 males and 10 females of Korean Native Ogolgyes, l82 days. The measured mean values, standard deviation and ranges of blood glucose,

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Effect of Arrowroot Flower (Puerariae flos) Extract on Lowering of Ethanol Concentration in Rat Blood (갈화(Puerariae flos)추출물이 Rat 혈중 Ethanol 농도에 미치는 영향)

  • Kim, Jeong-Han;Min, Sun-Sik;Kim, Sung-Hoon;Hong, Heu-Do;Kim, Jong-Soo;Kim, Soo-Un
    • Applied Biological Chemistry
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    • v.38 no.6
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    • pp.549-553
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    • 1995
  • Ethanol concentration in blood, brain and liver of rats was shown to be effectively lowered by arrowroot flower extract. The lowering effect for ethanol concentration in blood was maximum when measured after 1 hour from ethanol feeding. Hot water extract was more effective than 80% ethanol extract. The treatment of extract at 10 min. before ethanol feeding gave a better result than that at 10 min after or 1 hour before ethanol feeding. The ethanol concentration in brain and liver was lowered as found in the blood ethanol concentration. Acetaldehyde was not detected either in blood or the tissues. The optimal amount of the Puerariae flos was 55.7 mg/kg body weight. The newly developed analytical method using dichloromethane as extracting solvent was proven to be very effective in terms of speed and simplicity.

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Allogeneic Transplantation of Mesenchymal Stem Cells from Human Umbilical Cord Blood

  • Lee, Jae-Kwon
    • Journal of Applied Biological Chemistry
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    • v.50 no.4
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    • pp.187-195
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    • 2007
  • The cord blood serves as a vehicle for the transportation of oxygen and nutrients to the fetus. In the past, the human cord blood has generally been discarded after birth. However, numerous studies have described the regenerative ability of the cord blood cells in various incurable diseases. The umbilical cord blood (UCB)-derived stem cells are obtained through non-invasive methods that are not harmful to both the mother and the fetus. Furthermore, the cord blood stem cells are more immature than the adult stem cells and expand readily in vitro. The mesenchymal stem cells (MSCs) have the capacity to differentiate in vitro into various mesodermal (bone, cartilage, tendon, muscle, and adipose), endodermal (hepatocyte), and ectodermal (neurons) tissues. This review describes the immunological properties of the human UCB-MSCs to assess their potential usefulness in the allogeneic transplantation for the regenerative medicine.

Preparation of Blood Glue from Porcine Plasma Protein and Cross-linking Reaction of Plasma Protein with Formaldehyde

  • Cho, Yongsik;Lee, Hwahyoung;Song, Kyung Bin
    • Journal of Applied Biological Chemistry
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    • v.42 no.2
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    • pp.81-84
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    • 1999
  • Blood glue was prepared to reutilize porcine blood. Plasma proteins after lyophilization were treated by addition of wood flour, sodium hydroxide, sodium silicate, and hydrated lime to make blood glue with a suitable adhesivity. Characteristics of the prepared blood glue was monitored by measuring the viscosity with time, and the relationship between degree of hydrolysis of plasma proteins by addition of various amounts of sodium hydroxide and adhesivity was studied. To prevent the emission of formaldehyde during manufacturing of plywood by blood glue, the cross-linking reaction of plasma protein with formaldehyde was also examined. Fourier transform infrared, circular dichroism, and fluorescence spectroscopy study showed that blood plasma proteins react with formaldehyde, resulting in removal of formaldehyde by cross-linking reaction.

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Preparation of blood-compatible polyurethanes by surface modification (표면개질에 의한 혈액적합성 폴리우레탄의 제조)

  • Han, Dong-Keun;Jeong, Seo-Young;Kim, Young-Ha;Min, Byoung-Goo
    • Proceedings of the KOSOMBE Conference
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    • v.1990 no.11
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    • pp.61-63
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    • 1990
  • To develop better blood compatibility of commercial polyurethane(PU), PU surface was chemically modified wi th a hydrophobic perfluorocarhon or a hydrophilic polyethylene oxide(PEO) and/or sulfonated groups, respectively. The water contact angle of modified PUs varied from $110^{\circ}$ to $0^{\circ}$. All the modified PUs were more blood compatible than untreated PU. In particular, PEO-sulfonate grafted PUs showed a very enhanced antithrombogenicity due to the synergistic effect of PEO and $SO_3$ groups. Therefore more hydrophobic and hydrophilic PU surfaces are promising for improving the blood compatibility.

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Effects of clove oil on haematology and blood chemistry in crusian carp (Carassius auratus) (Clove Oil의 붕어(carassius auratus) 혈액 및 혈액화학치에 대한 영향)

  • Kho, Kyoung-Nam;Jung, Tae-Sung;Heo, Gang-Joon
    • Korean Journal of Veterinary Research
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    • v.47 no.4
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    • pp.483-488
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    • 2007
  • To determine the effects of anesthesia to clove oil on the secondary stress indices, changes in haematological and blood chemistry were monitored in healthy crucian carps (Carassius auratus). 24 fishes were divided into three groups, and blood was collected before anaesthesia (control group), immediately after anaesthesia at a concentration of 30 mg/l clove oil (anesthetized group), and 24 h after anaesthesia (recovered group). The anesthesia to clove oil significantly increased glucose in anaesthetized group, and constantly decreased lactate dehydrogenase in anaesthetized group and recovered group. However, clove oil had not effect on other biochemical indices. These results suggests that the anesthetic use of clove oil at a concentration of 30 mg/l does not cause irreversible damage in carp.