Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Reduced Autophagy in 5-Fluorouracil Resistant Colon Cancer Cells

  • Yao, Cheng Wen (School of Medicine No.1 and Institute for Nuclear Science and Technology, Jeju National University) ;
  • Kang, Kyoung Ah (School of Medicine No.1 and Institute for Nuclear Science and Technology, Jeju National University) ;
  • Piao, Mei Jing (School of Medicine No.1 and Institute for Nuclear Science and Technology, Jeju National University) ;
  • Ryu, Yea Seong (School of Medicine No.1 and Institute for Nuclear Science and Technology, Jeju National University) ;
  • Fernando, Pattage Madushan Dilhara Jayatissa (School of Medicine No.1 and Institute for Nuclear Science and Technology, Jeju National University) ;
  • Oh, Min Chang (School of Medicine No.1 and Institute for Nuclear Science and Technology, Jeju National University) ;
  • Park, Jeong Eon (School of Medicine No.1 and Institute for Nuclear Science and Technology, Jeju National University) ;
  • Shilnikova, Kristina (School of Medicine No.1 and Institute for Nuclear Science and Technology, Jeju National University) ;
  • Na, Soo-Young (School of Medicine No.2, Jeju National University) ;
  • Jeong, Seung Uk (School of Medicine No.2, Jeju National University) ;
  • Boo, Sun-Jin (School of Medicine No.2, Jeju National University) ;
  • Hyun, Jin Won (School of Medicine No.1 and Institute for Nuclear Science and Technology, Jeju National University)
  • Received : 2016.03.28
  • Accepted : 2016.07.19
  • Published : 2017.05.01
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Abstract

We investigated the role of autophagy in SNUC5/5-FUR, 5-fluorouracil (5-FU) resistant SNUC5 colon cancer cells. SNUC5/5-FUR cells exhibited low level of autophagy, as determined by light microscopy, confocal microscopy, and flow cytometry following acridine orange staining, and the decreased level of GFP-LC3 puncta. In addition, expression of critical autophagic proteins such as Atg5, Beclin-1 and LC3-II and autophagic flux was diminished in SNUC5/5-FUR cells. Whereas production of reactive oxygen species (ROS) was significantly elevated in SNUC5/5-FUR cells, treatment with the ROS inhibitor N-acetyl cysteine further reduced the level of autophagy. Taken together, these results indicate that decreased autophagy is linked to 5-FU resistance in SNUC5 colon cancer cells.

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References

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