점몰개 Squalidus multimaculatus(Gobioninae)의 난 발생 및 자치어 형태 발달

Morphological Development of Egg and Larvae of Squalidus multimaculatus (Gobioninae)

  • 송하윤 (국립수산과학원 중앙내수면연구소) ;
  • 고명훈 (이화여자대학교 에코과학부) ;
  • 서인영 (순천향대학교 생명시스템학과) ;
  • 문신주 (순천향대학교 생명시스템학과) ;
  • 방인철 (순천향대학교 생명시스템학과)
  • Song, Ha-Yoon (Inland Fisheries Research Institute, National Institute of Fisheries Science) ;
  • Ko, Myeong-Hun (Division of Ecoscience, Ewha Womans University) ;
  • Seo, In-Young (Department of Life Science, Soonchunhyang University) ;
  • Moon, Shin-Joo (Department of Life Science, Soonchunhyang University) ;
  • Bang, In-Chul (Department of Life Science, Soonchunhyang University)
  • 투고 : 2016.11.08
  • 심사 : 2017.02.10
  • 발행 : 2017.03.31

초록

한국고유어종 Squalidus multimaculatus의 난 발생 과정 및 초기 생활사를 연구하였다. 성숙된 암컷 친어에 HCG (human chorionic gonadotropin)를 10 IU/g의 농도로 주사하여 성숙란을 얻었으며, 습식법으로 인공수정하였다. 수정란의 직경은 0.8~0.9 mm로 원형으로 투명한 황색을 띈 침성 점착란이었으며, 유구는 없었다. 부화는 수온 평균 $24{\pm}1^{\circ}C$에서 수정 후 65시간을 전후하여 시작하였다. 부화자어는 전장이 2.5~3.1 m로 입과 항문은 열려있지 않았다. 부화 후 5일째 자어는 전장이 4.0~4.2 mm로 난황이 완전히 흡수되며 입과 항문이 완전히 열리면서 후기자어로 이행하였다. 부화 후 30일째 개체의 전장은 11.2~15.7 mm로 모든 지느러미의 기조수가 정수에 달하며 몸의 무늬가 어미와 유사한 치어 단계로 이행하였다. 부화 45일 후에는 전장이 18.8~22.5 mm로 자라 외형이 성어와 유사하였고, 80일 후에는 25.7~35.9 mm로 자라 외형과 반문이 성어와 동일하였다.

The egg development and early life history of Korean endemic fish, Squalidus multimaculatus (Gobioninae), were investigated. The eggs from the females were obtained by injecting 10 IU/g of human chorionic gonadotropin and inseminated by wet method in the laboratory. The fertilized eggs were 0.8~0.9 mm in diameter and had no oil globules. The embryo began to hatch about 65 hrs after fertilization under water temperature of $24{\pm}1^{\circ}C$. The newly-hatched larvae were 2.5~3.1 mm in total length, and their mouth and anus were not opened. Four days after hatching, the postlarva were 4.0~4.2 mm in total length, and their york sacs were completely absorbed. They entered the juvenile stage when all fin-rays were formed at 30 days after hatching, and their total length were 11.2~15.7 mm. At 45 days after hatching, the external from of juveniles were similar to those of adults (total length were 18.8~22.5 mm), and 80 days after hatching, the external characteristics from of juveniles were same to adults (total length were 25.7~35.9 mm).

키워드

참고문헌

  1. Back, Y.G. 1978. On the life history of Microphysogobio yaluensis (Mori). Korean J. Limnol., 11: 43-50.
  2. Balon, E.K. 1975. Terminology of intervals in fish development. J. Fish. Res. Board Can., 32: 1663-1670. https://doi.org/10.1139/f75-196
  3. Balon, E.K. 1985. Early Life Histories of Fishes: New evelopmental, ecological and evolutionary perspectives. Dr. W. Junk publisher., Dordrecht, 280pp.
  4. Blexter, J.H.S. 1974. The early life history of fish. Spring-Verlag. Berlin, 765pp.
  5. Byeon, H.K. 2012. Population ecology of Squalidus japonicus coreanus (Cyprinidae) in the Namhan River, Korea. Kor. J. Env. Eco., 26: 367-373. (in Korean)
  6. Cho, J.K., C.G. Hong, J.Y. Park, M.H. Son, C.K. Park and J.M. Park. 2015. Effects of water temperature and salinity on the egg development and larvae of sevenband grouper, Epinephelus septemfascuatus. Korean J. Ichthyol., 27: 21-25. (in Korean)
  7. Chyung, M.K. 1977. The Fishes of Korea. Ilji-sa., Korea, 727pp. (in Korean)
  8. Duarte, C.M and M. Alcaraz. 1989. To produce many small or few large eggs: a size-independent reproductive tactic of fish. Oecologia, 80: 401-404. https://doi.org/10.1007/BF00379043
  9. Han, K.H., D.S. Jin, D.J. Yoo, S.R. Baek and D.S. Hwang. 2001. The early life history of the crucian carp, Carassius auratus in Korea. Bull. Yuso Nat. Univ., 16: 379-389. (in Korean)
  10. Hosoya, K. and S.L. Jeon. 1984. A new cyprinid fish, Squalidus multimaculatus, from small rivers on the eastern slope of the Taebaik Mountain Chain, Korea. Korean J. Lim., 17: 41-49
  11. Jeon, S.L. and Y.J. Kim. 1998. First record of the Squalidus multimaculatus (Pisces: Cyprinidae) from Wangpi-river, Kyongsangbuk-do, Korea. J. Basic Sci., 11: 1-5. (in Korean)
  12. Kang, E.J. and C.H. Lee. 1996. Early life history of Korean bullhead, Pseudobagurus fulvidraco (Pisces, Bagridae), from Korea. Korean J. Ichthyol., 8: 83-89. (in Korean)
  13. Kim, C.H., W.S. Choi, D.H. Kim and J.M. Beak. 2014. Egg development and early life history of Korean endemic species, Acheilognathus majusculus (Acheilognathinae). Korean J. Ichthyol., 26: 17-24. (in Korean)
  14. Kim, I.S. 1997. Illustrated Encycolopedia of Fauna & Flora of Korea, Vol. 37, Freshwater Fishes. Ministry of Education, Yeongi, pp. 211-217. (in Korean)
  15. Kim, I.S. and J.Y. Park. 2002. Freshwater fishes of Korea. Kyohak Publishing Co. Ltd., Seoul, 449pp. (in Korean)
  16. Kim, I.S. and Y.J. Lee. 1984. Taxonomic review of genus Squalidus (Cyprinidae, Pisces), Bull. Korean Fish. Soc., 17: 132-138.
  17. Kim, I.S., Y. Choi, C.L. Lee, Y.J. Lee, B.J. Kim and J.H. Kim. 2005. Illustrated Book of Korean Fishes. Kyohak Publishing Co. Ltd., Seoul, 615pp. (in Korean)
  18. Ko, M.H., W.J. Kim, S.Y. Park and I.C. Bang. 2011a. Egg development and early life history of the endangered species Gobiobotia macrocephala (Cyprinidae). Korean J. Ichthyol., 23: 196-205. (in Korean)
  19. Ko, M.H., S.Y. Park, I.R. Lee and I.C. Bang. 2011b. Egg development and early life history of the endangered species Gobiobotia brevibarba (Pisces: Cyprinidae). Korean J. Limnol., 44: 136-143. (in Korean)
  20. Ko, M.H., S.Y. Park and I.C. Bang. 2012. Egg development and early life history of the Slender Shinner, Pseudopuntungia tenuicorpa (Pisces: Cyprinidae). Korean J. Ichthyol., 24: 48-55. (in Korean)
  21. Ko, M.H., S.J. Moon and I.C. Bang. 2013. Fish community structure and inhabiting status of endangered species in Babong stream. Korean J. Limnol., 46: 192-204. (in Korean) https://doi.org/10.11614/KSL.2013.46.2.192
  22. Ko, M.H. and Y.J. Won. 2016. Egg development and early life history of the Korean spin loach, Cobitis tetralineata (Pisces: Cobitidae). Korean J. Ichthyol., 27: 95-103. (in Korean)
  23. Lee, S.H., K.H. Han and D.S. Hwang. 2002. The early life history and spawning behavior of the Puntungia herzi. J. Inst. Sci. Yosu Natl Univ., 4: 115-126. (in Korean)
  24. Lee, S.H., K.H. Han, S.M. Yun, D.S. Hwang, D.J. Yu, C.R. Lee, I.S. Kim and Y.M. Son. 2004. Early life history and spawning behavior of Pseudopuntungia nigra. Korean J. Ichthyol., 16: 309-316. (in Korean)
  25. Lee, S.H., G.N. Oh, K.S. Kim, Y.S. Oh, K.W. Kang, J.H. Hwang, B.I. Lee, W.K. Lee and K.H. Han. 2008. Embryonic and larval development of goby minnow, Pseudogobio esocinus. Dev, Reprod., 12: 283-288. (in Korean)
  26. Lee, W.O., K.H. Kim, J.M. Beak, Y.J. Kang, H.Z. Jeon and C.H. Kim. 2011. Embryonic development and early life history of the northern loach, Cobitis pacifica (Pisces: Cobitidae). Korean J. Limnol., 44: 1-8. (in Korean)
  27. ME (Ministry of environment). 2009. Development of genetic diversity analysis, culture and ecosystem restoration techniques for endangered fish, Iksookimia choii. Soonchunhyang University, Asan, 506pp. (in Korean)
  28. MLTM (Ministry of Land & Transport and Maritime Affairs). 2010. Culture and Restoration of Endangered Species in the Major Four River Drainages. Soonchunhyang University, Asan, 489pp. (in Korean)
  29. MLTM (Ministry of Land & Transport and Maritime Affairs). 2010. Culture and Restoration of Endangered Species in the Major Four River Drainages (II). Soonchunhyang University, Asan, 364pp. (in Korean)
  30. Nakabo, T. 1993. Fishes of Japan: with pictorial keys to the species. Tokai University Press, Japan, pp. 230-231. (in Japanese)
  31. Nakamure, M. 1969. Cyprinid fishes of Japan. Research Institute of Natural Resources. pp. 105-106, 126-137.
  32. NIBR (National Institute of Biological Resources). 2013. Breeding Manual of Endangered Freshwater Fish. Incheon, 239pp. (in Korean).
  33. Park, J.M., H.S. Yim, Y.S. Lee, H.Y. Kim and K.H. Han. 2015. Egg development of the ussurian bullhead fish, Leiocassis ussuriensis (Pisces: Bagridae) morphological development of its larvae and juveniles. Dev. Reprod., 19: 189-196. (in Korean) https://doi.org/10.12717/DR.2015.19.4.189
  34. Park, J.M., S.M. Yoon, Y.S. Seo, K.H. Han and D.J. Yu. 2016. Morphological development of eggs, larvae and juveniles of the starlet sturgeon, Acipenser tuthenus (Pisces: Acipenseridae). Korean J. Ichthyol., 28: 179-185. (in Korean)
  35. Park, K.S., Y.P. Hong, U.K. Moon, S.S. Choi and K.K. An. 2005a. The egg development of Korean slender gudgeon, Squalidus gracilis majimae (Cypriniformes: Cyprinidae). Korean J. Limnol., 38: 73-82. (in Korean)
  36. Park, K.S., Y.P. Hong, S.S. Choi and K.K. An. 2005b. The spawning behavior of Korean slender gudgeon, Squalidus gracilis majimae (Cypriniformes: Cyprinidae). Korean J. Limnol., 38: 207-216. (in Korean)
  37. Soin, S.G., A.O. Kasutyan and N.I. Pashchenko. 1981. Ecological and morphological analysis of the development of the minnow, Phoxinus phoxinus (Cyprinidae). J. Ichthyol., 21: 90-105.
  38. Song, H.H. 1977. Studies on the spawning and early development of Coreoleuciscus splendidus Mori. J. Sci. Edu. Jeon-ju National University of Education, 3: 49-60. (in Korean)
  39. Song, H.Y., W.J. Kim, W.O. Lee and I.C. Bang. 2008. Morphological development of egg and larvae of Iksookimia choii (Cobitidae). Korean J. Limnol., 41: 104-110. (in Korean)
  40. Song, H.Y., H. Yang, E.M. Jo, H.C. Sin and I.C. Bang. 2009. Morphological development of egg and larvae of Koreocobitis nakdongensis (Cobitidae). Korean J. Ichthyol., 21: 247-252. (in Korean)
  41. Ware, D.M. 1975. Relation between egg size, growth, and natural mortality of larval fish. J. Fish. Res. Board Can., 32: 2503-2512. https://doi.org/10.1139/f75-288
  42. Wang, Q.Q., J.M. WU, F.T. Zhang and J.W. Wang. 2010. Early Development and Starvation Tolerance of the Larva of Squalidus argentatus in Chishui River. Chinese J. Zoology, 45: 11-20.