Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Cyanate Induces Apoptosis of Rat Glioma Cell Line

시안산에 의한 신경아교종세포의 자멸사

  • Choi, Hye-Jung (Department of Emergency Medical Technology, Gyeongbuk Provincial College) ;
  • Lee, Sang-Hee (Department of Emergency Medical Technology, Gyeongbuk Provincial College)
  • 최혜정 (경북도립대학교 응급구조과) ;
  • 이상희 (경북도립대학교 응급구조과)
  • Received : 2016.09.27
  • Accepted : 2017.02.17
  • Published : 2017.03.30
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Abstract

The patient with end-stage renal disease show several nervous complications. The factors contributing to the nervous complications are still incompletely characterized. Cyanate, known as one of the uremic toxins, is derived spontaneously from urea. To investigate the mechanism of cyanate-induced effect on C6 glioma cells, the glioma cells were treated with 0, 1, 5, 10, 20 and 40 mM cyanate. There was a dose-dependent decrease in cell viability and the decreased number of cell was observed in glioma cells by treatment with cyanate. Western blot showed the down- regulation of procaspase-3, which means up-regulation of caspase-3, and the up-regulation of caspase-8, but the down-regulation by cyanate. In addition, cDNA microarray showed 934 down-regulated genes and 165 up-regulated genes on 1,099 genes in cyanate treated group. Treatment with cyanate led to 16 down-regulated genes and 6 up-regulated genes on apoptosis category, and especially heat shock 70 kD protein 1A gene on the category of apoptosis was significantly up-regulated. These results suggest that cyanate can induce apoptosis through caspase-8 and caspase-3 in glioma cells and decrease of gene expression including apoptosis category in glioma cells. These effects of cyanate may play a role in the nervous complications of patient with end-stage renal disease.

본 연구는 말기 신부전 환자의 체내에서 증가되는 시안산이 신경학적 합병증의 원인으로 작용하는지 알아보고자 시안산 처리에 따른 신경아교종 세포인 C6 세포의 변화를 관찰하였다. 또한, 시안산에 의해 발현되는 세포자멸사 관련 인자를 알아보기 위하여 western blot 및 유전자 발현의 변화를 검색하기 위하여 cDNA 유전자 미세배열분석을 하였다. 시안산의 처리 농도가 0, 1, 5, 10, 20, 40 mM 증가할수록 신경아교종 세포의 생존율이 유의하게 감소하였고 세포자멸사에 주된 역할을 하는 caspase-8는 증가되었고 procaspase-3는 감소하였다. 그러나 caspase-8에 의해 활성화되는 Bax 단백질은 시안산의 처리 농도가 증가할수록 caspase-8의 증가에도 감소하였고, 세포자멸사를 조절하는 단백질인 Bcl-2와 IAP은 명확히 확인할 수 없었다. cDNA 유전자 미세배열 분석 결과, 총 1,099 종의 유전자 중에서 934 개의 유전자가 감소하였고 증가된 것은 165 개였다. 세포자멸사 관련 유전자에서도 감소한 것은 16 개였고, 증가된 6 개 유전자 가운데 heat shock 70 kD protein 1A가 현저한 증가를 나타내었다. 이상의 결과로 보아, 시안산은 신경아교종 세포에서 caspase-8 및 caspase-3와 관련된 세포자멸사를 유발시키며, 신경아교종 세포의 유전자들의 발현을 감소시키는 것으로 생각된다. 따라서 체내에서 증가된 시안산이 신경아교종 세포에 영향을 미쳐 말기 신부전 환자의 뇌병증에도 영향을 주는 것이라 생각된다.

Keywords

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