Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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A Comparison of the Microbial Diversity in Korean and Chinese Post-fermented Teas

한국과 중국 미생물 발효차의 미생물 군집분석 및 비교

  • Kim, Byung-Hyuk (Agricultural Research Institute for Climate Change, National Institute of Horticultural and Herbal Science, RDA) ;
  • Jang, Jong-Ok (Agricultural Research Institute for Climate Change, National Institute of Horticultural and Herbal Science, RDA) ;
  • Joa, Jae-Ho (Agricultural Research Institute for Climate Change, National Institute of Horticultural and Herbal Science, RDA) ;
  • Kim, Jin-Ah (Agricultural Research Institute for Climate Change, National Institute of Horticultural and Herbal Science, RDA) ;
  • Song, Seung-Yeob (Agricultural Research Institute for Climate Change, National Institute of Horticultural and Herbal Science, RDA) ;
  • Lim, Chan Kyu (Agricultural Research Institute for Climate Change, National Institute of Horticultural and Herbal Science, RDA) ;
  • Kim, Chun Hwan (Agricultural Research Institute for Climate Change, National Institute of Horticultural and Herbal Science, RDA) ;
  • Jung, Young Bin (Agricultural Research Institute for Climate Change, National Institute of Horticultural and Herbal Science, RDA) ;
  • Seong, Ki-Cheol (Agricultural Research Institute for Climate Change, National Institute of Horticultural and Herbal Science, RDA) ;
  • Kim, Hee-Sik (Cell Factory Research Center, KRIBB) ;
  • Moon, Doo-Gyung (Agricultural Research Institute for Climate Change, National Institute of Horticultural and Herbal Science, RDA)
  • 김병혁 (농촌진흥청 국립원예특작과학원 온난화대응농업연구소) ;
  • 장종옥 (농촌진흥청 국립원예특작과학원 온난화대응농업연구소) ;
  • 좌재호 (농촌진흥청 국립원예특작과학원 온난화대응농업연구소) ;
  • 김진아 (농촌진흥청 국립원예특작과학원 온난화대응농업연구소) ;
  • 송승엽 (농촌진흥청 국립원예특작과학원 온난화대응농업연구소) ;
  • 임찬규 (농촌진흥청 국립원예특작과학원 온난화대응농업연구소) ;
  • 김천환 (농촌진흥청 국립원예특작과학원 온난화대응농업연구소) ;
  • 정영빈 (농촌진흥청 국립원예특작과학원 온난화대응농업연구소) ;
  • 성기철 (농촌진흥청 국립원예특작과학원 온난화대응농업연구소) ;
  • 김희식 (한국생명공학연구원 세포공장연구센터) ;
  • 문두경 (농촌진흥청 국립원예특작과학원 온난화대응농업연구소)
  • Received : 2017.02.28
  • Accepted : 2017.03.23
  • Published : 2017.03.28
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Abstract

Tea is the most popular beverage in the world. The three main types are green, black, and post-fermented. Post-fermented teas are produced by the microbial fermentation of sun-dried green tea leaves (Camellia sinensis). In this study, the composition of the bacterial communities involved in the production of traditional oriental post-fermented teas (Korean algacha, dancha, and Chinese pu-erh) were investigated using 16S rRNA gene analysis. The dominant microorganisms present in the post-fermented teas included the ${\alpha}$-proteobacteria Rhodobacteraceae and Sphingomonas, and the ${\gamma}$-proteobacteria Pantoea. Cluster analysis confirmed that the microbial populations present in both Korean and Chinese post-fermented teas grouped into the same class. Interestingly, the dominant microorganism present in the Korean post-fermented teas was a bacterium, while for the Chinese post-fermented tea, it was a fungus.

차는 세계적으로 인기있는 음료로서, 그 종류는 불발효차(녹차), 반발효차(우롱차), 완전발효차(홍차), 후발효차 등으로 구분된다. 후발효차는 차나무(Camellia sinensis)의 잎을 미생물 발효과정을 거쳐 생산된다. 본 연구에서 한국 후발효차(알가차, 단차)와 중국 후발효차(보이차 2종)에 우점하는 미생물 분석을 위해 16S rRNA 유전자를 이용하였다. 후발효차에 우점하는 미생물은 ${\alpha}$-proteobacteria에 속하는 Rhodobacteraceae와 Sphingomonas, ${\gamma}$-proteobacteria에 속하는 Pantoea가 우점하였다. 미생물 군집 cluster 분석결과, 한국 후발효차와 중국 후발효차는 다르게 분류됨을 확인하였다. 또한, 매우 흥미롭게도 한국 후발효차는 미생물이 우점하였고 중국 후발효차는 곰팡이가 우점하는 것으로 분석되었다.

Keywords

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