Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Xylitol Production by Kluyveromyces marxianus 36907-FMEL1 at High Temperature was Considerably Increased through the Optimization of Agitation Conditions

교반속도 최적화를 통한 Kluyveromyces marxianus 36907-FMEL1로 부터 고온에서의 자일리톨 생산성 증진

  • Kim, Jin-Seong (Department of Bioengineering and Technology, Kangwon National University) ;
  • Park, Jae-Bum (Department of Bioengineering and Technology, Kangwon National University) ;
  • Jang, Seung-Won (Department of Bioengineering and Technology, Kangwon National University) ;
  • Kwon, Deok-Ho (Department of Bioengineering and Technology, Kangwon National University) ;
  • Hong, Eock Kee (Department of Bioengineering and Technology, Kangwon National University) ;
  • Shin, Won Cheol (Department of Bioengineering and Technology, Kangwon National University) ;
  • Ha, Suk-Jin (Department of Bioengineering and Technology, Kangwon National University)
  • Received : 2016.11.08
  • Accepted : 2017.01.26
  • Published : 2017.03.28
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Abstract

Recently, we isolated the mutant Kluyveromyces marxianus 36907-FMEL1, which demonstrated improved xylose reductase activity as compared to the parental strain, K. marxianus ATCC 36907. Effects of agitation conditions on xylitol production were verified using a bioreactor system. Under an agitation speed of 400 rpm, K. marxianus 36907-FMEL1 exhibited the highest xylitol yield (0.57 g/g) and productivity ($0.64g{\cdot}l^{-1}{\cdot}h^{-1}$) at $30^{\circ}C$. When the fermentation temperature was increased to $40^{\circ}C$, interestingly, xylitol yield and productivity were found to be increased to 21% (0.64 g/g) and 58% ($0.90g{\cdot}l^{-1}{\cdot}h^{-1}$), respectively, under the optimized agitation conditions.

최근 모균주인 K. marxianus ATCC 36907 보다 xylose reductase의 활성이 증대된 돌연변이 K. marxianus 36907-FMEL1 균주를 개발하였다. 본 연구에서는 효율적인 xylitol의 생산을 위해 발효기를 이용하여 교반속도에 따른 효과를 확인하였다. $30^{\circ}C$ 조건에서 발효를 진행한 결과 K. marxianus 36907-FMEL1 균주는 400 rpm의 교반속도에서 가장 높은 수율 (0.57 g/g)과 생산성($0.64g{\cdot}l^{-1}{\cdot}h^{-1}$)을 확인할 수 있었다. 흥미롭게도 온도 조건을 $40^{\circ}C$로 증가하여 발효를 진행한 결과 agitation 조건 최적화를 통해 수율과 생산성이 각각 21% (0.64 g/g)와 58% ($0.90g{\cdot}l^{-1}{\cdot}h^{-1}$) 증가하였다.

Keywords

References

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