Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Functional Characteristics of Enterococcus faecium SA5 and Its Potential in Conversion of Ginsenoside Rb1 in Ginseng

Enterococcus faecium SA5의 기능적 특성과 인삼 ginsenoside Rb1의 전환

  • Kim, Eun-Ah (Seoul F&B Company) ;
  • Renchinkhand, Gereltuya (Division of Animal Resources Science, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Urgamal, Magsal (Division of Animal Resources Science, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Park, Young W. (Agricultural Research Station, Fort Valley State University) ;
  • Nam, Myoung Soo (Division of Animal Resources Science, College of Agriculture and Life Sciences, Chungnam National University)
  • 김은아 (서울F&B) ;
  • 랜친핸드 (충남대학교 농업생명과학대학 동물자원과학부) ;
  • 어르가말 막살 (충남대학교 농업생명과학대학 동물자원과학부) ;
  • 박영우 (포트벨리 주립대학교 농업연구본부) ;
  • 남명수 (충남대학교 농업생명과학대학 동물자원과학부)
  • Received : 2016.08.23
  • Accepted : 2017.02.18
  • Published : 2017.02.28
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Abstract

The fermentation of Panax ginseng can yield many compounds from ginsenosides that have a wide variety of biological functions. Lactic acid bacteria (LAB) strains are capable of converting ginsenosides. The purposes of this study were to: (1) characterize Enterococcus faecium SA5, an isolated LAB from Mongolian mare milk, (2) identify the existence of extracellular ${\beta}$-glucosidase activity in the milk, and (3) ascertain if the ${\beta}$-glucosidase has the capacity of converting ginsenoside in Korean ginseng. The results revealed that E. faecium SA5 was acid-resistant, bile salt-resistant, and has antibiotic activities against 4 pathogenic microorganisms (Salmonella typhimurium KCTC 3216, Listeria monocytogenes KCTC 3710, Bacillus cereus KCTC 1012, Staphylococcus aureus KCTC 1621). In addition, E. faecium SA5 had tolerance against some antibiotics such as colistin, gentamycin and neomycin. It was also found that E. faecium SA5 possessed bile salt hydrolase activity, which could lower blood cholesterol level. When incubated in 10% (w/v) skim milk as a yogurt starter, E. faecium SA5 caused to decrease pH of the medium as well as increase in viable cell counts. Using TLC and HPLC analysis on the samples incubated in MRS broth, our study confirmed that E. faecium SA5 can produce ${\beta}$-glucosidase, which was capable of converting ginsenoside $Rb_1$ into new ginsenosides $Rg_3-s$ and $Rg_3-r$. It was concluded that E. faecium SA5 possessed a potential of probiotic activity, which could be applied to yogurt manufacture as well as ginsenoside conversion in ginseng.

본 연구는 몽골 마유로부터 분리한 유산균 Enterococcus faecium SA5의 이화학 특성을 파악하고 유산균 E. faecium SA5의 ${\beta}$-glucosidase의 활성과 이를 통한 ginsenoside 전환을 확인하는 것을 목표로 진행되었다. E. faecium SA5는 내산성, 내담즙성을 나타내었으며 4종의 병원성 미생물(Salmonella typhimurium KCTC 3216, Listeria monocytogenes KCTC 3710, Bacillus cereus KCTC 1012, Staphylococcus aureus KCTC 1621)에 항균 활성을 가질 뿐만 아니라 항생물질 colistin, gentamycin, neomycin에 내성을 나타내었다. 또한, E. faecium SA5는 bile salt hydrolase 활성을 나타내어 혈액 내 콜레스테롤 수준 감소 효과가 있다고 사료되며 10% skim milk에서 배양하였을 때, pH가 감소하고 산도 및 생균수가 증가하는 것으로 보아 발효유 스타터로써의 활성을 갖는 것으로 판단되었다. 또한 E. faecium SA5의 ${\beta}$-glucosidase에 의해 ginsenoside $Rb_1$이 ginsenoside $Rg_3-s$와 $Rg_3-r$으로 전환되었음을 TLC 분석을 통해 확인하였다. 따라서 E. faecium SA5는 잠재적인 probiotics로 이를 이용하여 발효유 제조 및 ginsenoside 전환 관련 건강기능식품 개발에 응용할 수 있을 것으로 사료된다.

Keywords

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