Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
권호 표지
DOI QR코드

DOI QR Code

Antioxidant and Tyrosinase Inhibition Activity Promoting Effects of Perilla by the Light Emitting Plasma

발광플라즈마 처리에 의한 들깨 부위별 항산화 및 Tyrosinase 저해 활성 효과

  • Yoo, Ji Hye (Bioherb Research Institute, Kangwon National University) ;
  • Choi, Jae Hoo (Department of Bio-Resource Sciences, Kangwon National University) ;
  • Kang, Byeong Ju (Department of Bio-Resource Sciences, Kangwon National University) ;
  • Jeon, Mi Ran (Department of Bio-Resource Sciences, Kangwon National University) ;
  • Lee, Chan Ok (Bioherb Research Institute, Kangwon National University) ;
  • Kim, Chang Heum (Department of Bio-Resource Sciences, Kangwon National University) ;
  • Seong, Eun Soo (Department of Medicinal Plant, Suwon Women's University) ;
  • Heo, Kweon (Bioherb Research Institute, Kangwon National University) ;
  • Yu, Chang Yeon (Department of Bio-Resource Sciences, Kangwon National University) ;
  • Choi, Seon Kang (Department of Agricultural Life Sciences, Kangwon National University)
  • 유지혜 (강원대학교 한방바이오연구소) ;
  • 최재후 (강원대학교 생물자원과학과) ;
  • 강병주 (강원대학교 생물자원과학과) ;
  • 전미란 (강원대학교 생물자원과학과) ;
  • 이찬옥 (강원대학교 한방바이오연구소) ;
  • 김창흠 (강원대학교 생물자원과학과) ;
  • 성은수 (수원여자대학교 약용식물과) ;
  • 허권 (강원대학교 한방바이오연구소) ;
  • 유창연 (강원대학교 생물자원과학과) ;
  • 최선강 (강원대학교 농생명산업학과)
  • Received : 2017.01.03
  • Accepted : 2017.02.16
  • Published : 2017.02.28
Download PDF
⟨ Previous Next ⟩

Abstract

Background: The light emitting plasma (LEP) has recently attracted attention as a novel artificial light source for plant growth and functional component enhancement. We investigated the effects of LEP on whitening and antioxidant activities of the plant parts of perilla. Methods and Results: Previously germianted seeds of perilla were cultivated under different light conditions (fluoresce lamp, LED red, blue, white, green, and LEP) in a culture room for 2 months. Parts of perilla were harvested and extracted in 70% EtOH. The extracts were used to detect total phenolic contents, total flavonoid contents, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), reducing power and tyrosinase inhibition activity as indicators of biological activity. Biological activity was highest in seedlings grown under LEP. The total phenolic content was highest in the stems and the total flavonoid content was highest in the roots of perilla exposed to LEP. The DPPH and ABTS radical activity in all the parts of perilla exposed to LEP were higher by approximately three-fold compared to that in the control (fluoresce lamp). The reducing power values of perilla significantly increased after treatment with LEP. In addition, all the extract of perilla plants exposed to LEP promoted the tyrosinase inhibitory activity. These results suggest that LEP can be an important artificial light source for enhancement of biological activity. Conclusions: LEP could promote whitening and antioxidant activity of perilla.

Keywords

References

  1. Bernard P and Berthon JY. (2000). Resveratrol: An original mechanism on tyrosinase inhibition. International Journal of Cosmetic Science. 22:219-226. https://doi.org/10.1046/j.1467-2494.2000.00019.x
  2. Blois MS. (1958). Antioxidant determinations by the use of a stable free radical. Nature. 181:1199-1200. https://doi.org/10.1038/1811199a0
  3. Choi JH. (2015). Growth characteristics, physiological activities and gene expression related flavonoid biosynthesis by artificial light sources treatment in Astragalus membranaceus. Master Thesis. Kangwon National University. Korea. p.20-39.
  4. Choung MG. (2005). Comparison of major characteristics between seed perilla and vegetable perilla. Korean Journal of Crop Science. 50:171-174.
  5. Duval B and Shetty K. (2001). The stimulation of phenolics and antioxidant activity in pea(Pisum sativum) elicited by genetically transformed anise root extract. Journal of Food Biochemistry. 25:361-377. https://doi.org/10.1111/j.1745-4514.2001.tb00746.x
  6. Ha TH, Lee JH, Lee MH, Lee BW, Kwon HS, Park CH, Shim KB, Kim HT, Baek IY and Jang DS. (2012). Isolation and identification of phenolic compounds from the seeds of Perilla frutescens L. and their inhibitory activities against ${\alpha}$-glucosidase and aldose reductase. Food Chemistry. 135:1397-1403. https://doi.org/10.1016/j.foodchem.2012.05.104
  7. Jang IB, Yu J, Kweon KB and Suh SJ. (2016). Effect of controlled light environment on the growth and ginsenoside content of Panax ginseng C. A. Meyer. Korean Journal of Medicinal Crop Science. 24:277-283. https://doi.org/10.7783/KJMCS.2016.24.4.277
  8. Kim JY, Oh KY, Kim JY, Ryu HW, Jeong TS and Park KH. (2010). Polyphenols displaying tyrosinase inhibition from the seed of Psoralea corylifolia. Journal of the Korean Society for Applied Biological Chemistry. 53:427-432. https://doi.org/10.3839/jksabc.2010.066
  9. Kopsell DA and Sams CE. (2013). Increases in shoot tissue pigments, glucosinolates, and mineral elements in sprouting broccoli after exposure to short-duration blue light from light emitting diodes. Journal of the American Society for Horticultural Science. 138:31-37.
  10. Lee CO, Li CH, Lim JD and Yu CY. (2004). Regeneration ability in germplasms of Perilla frutescens. Korean Journal of Medicinal Crop Science. 12:500-507.
  11. Lee HJ. (2013). Effects of artificial light sources on the growth and quality of butterhead lettuce. Master Thesis. University of Seoul, Korea. p.19-40.
  12. Lee JH, Park KH, Lee MH, Kim HT, Seo WD, Kim JY, Baek IY, Jang DS and Ha TJ. (2013). Identification, characterisation, and quantification of phenolic compounds in the antioxidant activity-containing fraction from the seeds of Korean perilla (Perilla frutescens) cultivars. Food Chemistry. 136:843-852. https://doi.org/10.1016/j.foodchem.2012.08.057
  13. Lee KH, Park HS, Yoon IJ, Shin YB, Baik YC, Kooh DH, Kim SK, Jung HK, Sim MO, Cho HW, Jung WS and Kim MS. (2016). Whitening and anti-wrinkle effects of Tremella fuciformis extracts. Korean Journal of Medicinal Crop Science. 24:38-46. https://doi.org/10.7783/KJMCS.2016.24.1.38
  14. Lee SO, Lee HJ, Yu MH, Im HG and Lee IS. (2005). Total polyphenol contents and antioxidant activities of methanol extracts from vegetables produced in Ullung island. Korean Journal of Food Science and Technology. 37:233-240.
  15. Moreno MIN, Isla MI, Sampietro AR and Vattuone MA. (2000). Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. Journal of Ethnopharmacology. 71:109-114. https://doi.org/10.1016/S0378-8741(99)00189-0
  16. Oyaizu M. (1986). Studies on products of browning reactions: Antioxidative activities of products of browning reaction prepared from glucosamine. Japanese Journal of Nutrition. 44:307-315. https://doi.org/10.5264/eiyogakuzashi.44.307
  17. Park JE, Park YG, Jeong BR and Hwang SJ. (2012). Growth and anthocyanin content of lettuce as affected by artificial light source and photoperiod in a closed-type plant production system. Korean Journal of Horticultural Science and Technology. 30:673-679. https://doi.org/10.7235/hort.2012.12020
  18. Park WK, Park BH and Park YH. (2000). Encyclopedia of food and food science. Shinkang Publisher. Seoul, Korea. p.234.
  19. Perez Balibrea S, Moreno DA and Garcia Viguera C. (2008). Influence of light on health-promoting phytochemicals of broccoli sprouts. Journal of the Science of Food and Agriculture. 88:904-910. https://doi.org/10.1002/jsfa.3169
  20. Petrillo AD, Gonzalez-Paramas AM, Era B, Medda R, Pintus F, Santos-Buelga C and Fais A. (2016). Tyrosinase inhibition and antioxidant properties of Asphodelus microcarpus extracts. BMC Complementary and Alternative Medicine. 16:453-453. https://doi.org/10.1186/s12906-016-1442-0
  21. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M and Rice Evans C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine. 26:1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3
  22. Rocha RJM, Pimentel T, Serodio J, Rosa R and Calado R. (2013). Comparative performance of light emitting plasma(LEP) and light emitting diode(LED) in ex situ aquaculture of scleractinian corals. Aquaculture. 402:38-45.
  23. Seong ES, Hwang IS, Choi JH, Lee JG, Yoo JH, Ahn YS, Park CB and Yu CY. (2015a). Enhanced biomass and biological activity of 'Hasuo'(Polygonum multiflorum Thunberg) grown under LED light. Australian Journal of Crop Science. 9:168-174.
  24. Seong ES, Seo EW, Chung IM, Kim MJ, Kim HY, Yoo JH, Choi JH, Kim NJ and Yu CY. (2015b). Growth characteristics and phenol compounds analysis of collected Perilla frutescens resources from China and Japan. Korean Journal of Medicinal Crop Science. 23:132-137. https://doi.org/10.7783/KJMCS.2015.23.2.132
  25. Singleton VL and Rossi JA. (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture. 16:144-158.
  26. Tan JBL and Lim YY. (2015). Antioxidant and tyrosinase inhibition activity of the fertile fronds and rhizomes of three different Drynaria species. BMC Research Notes. 8:468-468. https://doi.org/10.1186/s13104-015-1414-3
  27. Wongnok A, Piluek C, Techasilpitak T and Tantivivat S. (2008). Effects of light emitting diodes on micropropagation of Phalaenopsis orchids. Acta Horticulturae. 788:149-156.

Cited by

  1. Enhancement of Growth Characteristics and Biological Activities in Astragalus membranaceus Using Artificial Light Sources vol.65, pp.5, 2018, https://doi.org/10.1134/S1021443718050059