Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
권호 표지
DOI QR코드

DOI QR Code

Evaluation of Anti-Oxidant and Anti-Inflammatory Activities of Ganoderma lucidum Cultured on Hulled Barley

겉보리에서 배양한 영지버섯 추출물의 항산화 및 항염증 효능 평가

  • Seo, Kyoung Hee (Department of Applied Biochemistry, Konkuk University) ;
  • Kim, Yeon Hwa (Department of Applied Biochemistry, Konkuk University) ;
  • Lee, Young Min (Department of Applied Biochemistry, Konkuk University) ;
  • Ghosh, Mithun (Department of Applied Biochemistry, Konkuk University) ;
  • Park, Kang Min (Department of Applied Biochemistry, Konkuk University) ;
  • Park, Dong Hyun (Department of Applied Biochemistry, Konkuk University) ;
  • Kim, Jin Seong (Department of Applied Biochemistry, Konkuk University) ;
  • Lim, Beong Ou (Department of Applied Biochemistry, Konkuk University)
  • 서경희 (건국대학교 응용생화학과) ;
  • 김연화 (건국대학교 응용생화학과) ;
  • 이영민 (건국대학교 응용생화학과) ;
  • ;
  • 박강민 (건국대학교 응용생화학과) ;
  • 박동현 (건국대학교 응용생화학과) ;
  • 김진성 (건국대학교 응용생화학과) ;
  • 임병우 (건국대학교 응용생화학과)
  • Received : 2016.11.15
  • Accepted : 2017.02.07
  • Published : 2017.02.28
Download PDF
⟨ Previous Next ⟩

Abstract

Background: Ganoderma lucidum cultured on hulled barley was investigated as a potential natural source of antioxidants and anti-inflammatory agents. Methods and Results: The yields from Ganoderma lucidum cultured on hulled barley water and ethanol extract were 17.69% and 25.77%, respectively. The antioxidant activity of Ganoderma lucidum cultured on hulled barley extracts was confirmed by various methods including assayss of 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis-(3-ethylbenzo thiazoline-6-sulfonic acid) (ABTS), nitrite radical scavenging, and $Fe^{3+}$ to $Fe^{2+}$ reducing power activity. The ethanol extract of Ganoderma lucidum cultured on hulled barley showed improved DPPH, ABTS and nitrite radical scavenging activity compared with the water extract. After treatment of RAW264.7 cells with Ganoderma lucidum cultured on hulled barley ethanol extracts, the cell viability compared with the control was 92.82%, even at a concentration of $3,000{\mu}g/m{\ell}$. The ethanol extract inhibited reactive oxygen species (ROS) generation in RAW264.7 cells stimulated with $H_2O_2$, even at low concentrations. In addition, the ethanol extract showed an inhibitory effects on the production of lipopolysaccharide-induced nitric oxide (NO) in RAW264.7 cells. Conclusions: This study suggests that the extract of Ganoderma lucidum cultured on hulled barley is a potential source of natural antioxidants and anti-inflammatory agents.

Keywords

References

  1. Adedapo AA, Jimoh FO, Koduru S, Masika PJ and Afolayan AJ. (2008). Evaluation of the medicinal potentials of the methanol extracts of the leaves and stems of Halleria lucida. Bioresource Technology. 99:4158-4163. https://doi.org/10.1016/j.biortech.2007.08.066
  2. Bohr VA. (2002). Repair of oxidative DNA damage in nuclear and mitochondrial DNA, and some changes with aging in mammalian cells. Free Radical Biology and Medicine. 32:804-812. https://doi.org/10.1016/S0891-5849(02)00787-6
  3. Cho SM, Park JS, Kim KP, Cha DY, Kim HM and Yoo ID. (1999). Chemical features and purification of immunostimulating polysaccharides from the fruit bodies of Agaricus blazei. The Korean Journal of Mycology. 27:170-174.
  4. Choi DJ, Lee SJ, Kang MJ, Cho HS, Sung NJ and Shin JH. (2008). Physicochemical characteristics of black garlic(Allium sativum L.). Journal of the Korean Society of Food Science and Nutrition. 37:465-471. https://doi.org/10.3746/jkfn.2008.37.4.465
  5. Choi Y, Lee SM, Chun J, Lee HB and Lee J. (2006). Influence of heat treatment on the antioxidant activities and polyphenolic compounds of shiitake(Lentinus edodes) mushroom. Food Chemistry. 99:381-387. https://doi.org/10.1016/j.foodchem.2005.08.004
  6. Chung YC, Chen SJ, Hsu CK, Chang CT and Chou ST. (2005). Studies on the antioxidative activity of Graptopetalum paraguayense E. Walther. Food Chemistry. 91:419-424. https://doi.org/10.1016/j.foodchem.2004.06.022
  7. Delanty N and Dichter MA. (1998). Oxidative injury in the nervous system. Acta Neurologica Scandinavica. 98:145-153. https://doi.org/10.1111/j.1600-0404.1998.tb07285.x
  8. Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS and Tannenbaum SR. (1982). Analysis of nitrate, nitrite, and [$^{15}N$] nitrate in biological fluids. Analytical Biochemistry. 126:131-138. https://doi.org/10.1016/0003-2697(82)90118-X
  9. Gulcina I, Oktayb M, Kireccic E and Kufrevioglu OI. (2003). Screening of antioxidant and antimicrobial activities of anise(Pimpinella anisum L.) seed extracts. Food Chemistry. 83:371-382. https://doi.org/10.1016/S0308-8146(03)00098-0
  10. Hong IJ, Park HG, Jew SS, Kim KT and Lee SH. (2003). Functional characteristics from the barley leaves and its antioxidant mixture: Study on the nitrite scavenging effect. Applied Biological Chemistry. 46:333-337.
  11. Hu C and Kitts DD. (2000). Studies on the antioxidant activity of Echinacea root extract. Journal of Agricultural and Food Chemistry. 48:1466-1472. https://doi.org/10.1021/jf990677+
  12. Huang HZ, Zhang CH, Yang EY, Lee SG, Choi KY and Yun HK. (2007). Effect of nutrient solution for hydroponics of Liliaceae leaf vegetables on the amount of ascorbic acid in Chinese chive. Journal of Bio-Environment Control. 16:222-227.
  13. Hyun JN, Kweon SJ, Park DS, Ko JM, Han SI, Lim SG and Suh SJ. (2008). A new high quality and yielding barley variety “Geungangbori” with lodging resistance. Korean Journal of Breeding Science. 40:474-478.
  14. Jin JH, Kim JS, Kang SS, Son KH, Chang HW and Kim HP. (2010). Anti-inflammatory and anti-arthritic activity of total flavonoids of the roots of Sophora flavescens. Journal of Ethnopharmacology. 127:589-595. https://doi.org/10.1016/j.jep.2009.12.020
  15. Jo SH, Cho CY, Ha KS, Choi EJ, Kang YR and Kwon YI. (2013). The antioxidant and antimicrobial activities of extracts of selected barley and wheat inhabited in Korean peninsula. Journal of the Korean Society of Food Science and Nutrition. 42:1003-1007. https://doi.org/10.3746/jkfn.2013.42.7.1003
  16. Jun DH, Kim HY, Han SI, Kim YH, Kim SG and Lee JT. (2013). Studies on antioxidant effect of mushroom complex. Journal of Life Science. 23:377-382. https://doi.org/10.5352/JLS.2013.23.3.377
  17. Kalra S and Jood S. (2000). Effect of dietary barley ${\beta}$-glucan on cholesterol and lipoprotein fractions in rat. Journal of Cereal Science. 31:141-145. https://doi.org/10.1006/jcrs.1999.0290
  18. Kim DH, Park SR, Debnath T, Hasnat M, Pervin M and Lim BO. (2013). Evaluation of the antioxidant activity and anti-inflammatory effect of Hericium erinaceus water extracts. Korean Journal of Medicinal Crop Science. 21:112-117. https://doi.org/10.7783/KJMCS.2013.21.2.112
  19. Kim MC, Kim JS and Heo MS. (2008). Antibacterial, antioxidant and antitumor activities of mushroom mycelium mixed culture extracts. Korean Society for Biotechnology and Bioengineering Journal. 23:158-163.
  20. Kim MJ and Park EJ. (2011). Feature analysis of different in vitro antioxidant capacity assays and their application to fruit and vegetable samples. Journal of the Korean Society of Food Science and Nutrition. 40:1053-1062. https://doi.org/10.3746/jkfn.2011.40.7.1053
  21. Kim YJ and Son DY. (2014). Inflammatory mediator regulation of the Zizyphus jujube leaf fractions in the LPS-stimulated RAW264.7 mouse machrophage. Korean Journal of Food Preservation. 21:114-120. https://doi.org/10.11002/kjfp.2014.21.1.114
  22. Klein BP and Perry AK. (1982). Ascorbic acid and vitamin a activity in selected vegetables from different geographical areas of the united states. Journal of Food Science. 47:941-945. https://doi.org/10.1111/j.1365-2621.1982.tb12750.x
  23. Ku KM, Kim SK and Kang YH. (2009). Antioxidant activity and functional components of corn silk(Zea mays L.). Korean Journal of Plant Resources. 22:323-329.
  24. Lee HJ, Lee SW, Park CG, Ahn YS, Kim JS, Bang MS, Oh CH and Kim CT. (2015). Effects of white Habiscus syriacus L. flower extracts on antioxidant activity and bone resorption inhibition. Korean Journal of Medicinal Crop Science. 23:190-197. https://doi.org/10.7783/KJMCS.2015.23.3.190
  25. Lee JM and Ahn MS. (1997). A study on nitrite scavenging ability of tea extracts. Journal of the Korean Society of Food Culture. 12:567-572.
  26. Nguyen TK, Shin DB, Lee SM, Im KH, Lee TS and Lee UY. (2013). Antioxidant and anti-inflammatory activities of methanol and hot water extracts of Pholiota nameko fruiting bodies. The Korean Journal of Mycology. 41:97-103. https://doi.org/10.4489/KJM.2013.41.2.97
  27. Park MY, Yu CG and Park YH. (2016). Effects of roasting and peeling process and extraction temperature on the antioxidant activity of burdock tea. Korean Journal of Medicinal Crop Science. 24:351-359. https://doi.org/10.7783/KJMCS.2016.24.5.351
  28. Park YJ, Nam JY, Yoon DE, Kwon OC, Kim HI, Yoo YB, Kong WS and Lee CS. (2013). Comparison of anti-inflammatory, antioxidant and anti-allergic effects of Ganoderma species mycelial extracts. Journal of Mushroom. 11:111-115. https://doi.org/10.14480/JM.2013.11.2.111
  29. Sato M, Ramarathnam N, Suzuki Y, Ohkubo T, Takeuchi M and Ochi H. (1996). Varietal differences in the phenolic content and superoxide radical scavenging potential of wines from different sources. Journal of Agricultural and Food Chemistry. 44:37-41. https://doi.org/10.1021/jf950190a
  30. Seitz HK and Stickel F. (2006). Risk factors and mechanisms of hepatocarcinogenesis with special emphasis on alcohol and oxidative stress. Biological Chemistry. 387:349-360.
  31. Seo KH, Lee JY, Debnath T, Kim YM, Park JY, Kim YO, Park SJ and Lim BO. (2016). DNA protection and antioxidant potential of chestnut shell extracts. Journal of Food Biochemistry. 40:20-30. https://doi.org/10.1111/jfbc.12179
  32. Shin YK, Jang HS, Kim JS, Ryu HY, Kim JK, Kwun IS and Sohn HY. (2008). Solid fermentation of medicinal herb using Phellinus baumii mycelium and anti-thrombin and antioxidation activity of its methanol extract. Korean Journal of Microbiology and Biotechnology. 36:201-208.
  33. Song ES, Park SJ, Woo NRA, Won MH, Cho JS, Kim JG and Kang MH. (2005). Antioxidant capacity of colored barley extracts by varieties. Journal of the Korean Society of Food Science and Nutrition. 34:1491-1497. https://doi.org/10.3746/jkfn.2005.34.10.1491
  34. Tamagawa K, Iizuka S, Fukushima S, Endo Y and Komiyama Y. (1997). Antioxidative activity of polyphenol extracts from barley bran. Journal of the Japanese Society for Food Science and Technology. 44:512-515. https://doi.org/10.3136/nskkk.44.512
  35. Wettasinghe M and Shahidi F. (1999). Antioxidant and free radical-scavenging properties of ethanolic extracts of defatted borage(Borago officinalis L.) seeds. Food Chemistry. 67:399-414. https://doi.org/10.1016/S0308-8146(99)00137-5
  36. Yeon SH, Ham HM, Sung JH, Kim YH, Namkoong SG, Jeong HS and Lee JS. (2013). Antioxidant activities of hot water extract from cornus walteri wanger against oxidative stress induced by tert-butyl hydroperoxide in HepG2 cells. Journal of the Korean Society of Food Science and Nutrition. 42:1525-1532. https://doi.org/10.3746/jkfn.2013.42.10.1525
  37. Yoon KK, Moon KG, Kim SU, Um IS, Cho YS, Kim YG and Rho IR. (2016). Analysis of growth and antioxidant compounds in deodeok in response to mulching materials. Korean Journal of Medicinal Crop Science. 24:183-190. https://doi.org/10.7783/KJMCS.2016.24.3.183

Cited by

  1. Application of Near Infrared Reflectance Spectroscopy for Rapid and Non-Destructive Discrimination of Hulled Barley, Naked Barley, and Wheat Contaminated with Fusarium vol.18, pp.2, 2018, https://doi.org/10.3390/s18010113