Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Conditions of In Vitro Spore Germination and Prothallium Culture for Sporophyte propagation of Polystichum braunii (Spenn.) Fée

좀나도히초미(Polystichum braunii (Spenn.) Fée) 포자체 증식을 위한 기내 포자 발아와 전엽체 배양 조건

  • Kwon, Hyuk Joon (National Institute of Biological Resources) ;
  • Han, Ji Hyun (Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural, and Food Sciences, Chungbuk National University) ;
  • Lee, Cheol Hee (Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural, and Food Sciences, Chungbuk National University) ;
  • Kim, Soo-Young (National Institute of Biological Resources)
  • 권혁준 (국립생물자원관) ;
  • 한지현 (충북대학교 축산.원예.식품공학부 생물건강소재산업화사업단) ;
  • 이철희 (충북대학교 축산.원예.식품공학부 생물건강소재산업화사업단) ;
  • 김수영 (국립생물자원관)
  • Received : 2017.07.26
  • Accepted : 2017.10.20
  • Published : 2017.12.31
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Abstract

This study was conducted to investigate the optimal conditions for spore germination, prothallus propagation, sporophyte formation, and seedling growth in Polystichum braunii (Spenn.) $F{\acute{e}}e$. The rate of spore germination and early prothalium development was high in Knop (41.2%), which had low mineral content. The optimal medium for prothallus propagation and sexual organ formation was 2MS medium (2% sucrose). Among the various mixtures of cultivation soil (bedding soil, peat moss, perlite, and decomposed granite), a mixture of bedding soil and decomposed granite at a ratio of 2:1 (v:v) had a positive effect on sporophyte formation (276.3 ea/$7.5m^2$). The most efficient conditions for promoting the growth of sporophytes were pots filled with only bedding soil.

본 연구는 좀나도히초미(Polystichum braunii (Spenn.) $F{\acute{e}}e$)의 효율적인 증식방법을 규명하기위해 기내 포자발아, 전엽체 증식, 기회 포자체 형성 등 단계별 배양조건을 찾고자 수행되었다. 포자 발아율과 초기 전엽체 발달은 무기물 함량이 적은 Knop 배지에서 41.2%로 가장 높았다. 전엽체의 증식 및 생식기관 형성을 위한 배지는 2% sucrose가 포함된 2 MS 배지였다. 또한 배양토 종류와 혼합비율을 달리하여 전엽체를 기외 이식한 결과, 원예용 상토와 마사토를 2:1 혼합한 배양토에서 재배하였을 때 포자체 형성이 276.3개/$7.5m^2$로 가장 많았다. 반면, 피트모스가 포함된 조건에서는 전엽체 증식 및 포자체 형성이 억제되었다. 포자체 육묘는 상토 단용처리구에서 재배하였을 때 생육이 가장 왕성하였다.

Keywords

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