Clinical and Experimental Reproductive Medicine

  • 제44권4호
  • /
  • Pages.201-206
  • /
  • 2017
  • /
  • 2233-8233(pISSN)
  • /
  • 2233-8241(eISSN)
대한생식의학회 (The Korean Society for Reproductive Medicine)
권호 표지
DOI QR코드

DOI QR Code

Sperm DNA fragmentation and sex chromosome aneuploidy after swim-up versus density gradient centrifugation

  • Kim, Sung Woo (Department of Obstetrics and Gynecology, Seoul National University Hospital) ;
  • Jee, Byung Chul (Department of Obstetrics and Gynecology, Seoul National University College of Medicine) ;
  • Kim, Seul Ki (Department of Obstetrics and Gynecology, Seoul National University College of Medicine) ;
  • Kim, Seok Hyun (Department of Obstetrics and Gynecology, Seoul National University Hospital)
  • 투고 : 2017.03.20
  • 심사 : 2017.09.20
  • 발행 : 2017.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Objective: The aim of this study was to compare the efficacy of swim-up and density gradient centrifugation (DGC) for reducing the amount of sperm with fragmented DNA, sex chromosome aneuploidy, and abnormal chromatin structure. Methods: Semen samples were obtained from 18 healthy male partners who attended infertility clinics for infertility investigations and were processed with swim-up and DGC. The percentages of sperm cells with fragmented DNA measured by the sperm chromatin dispersion test, normal sex chromosomes assessed by fluorescence in situ hybridization, and abnormal chromatin structure identified by toluidine blue staining were examined. Results: The percentage of sperm cells with fragmented DNA was significantly lower in the swim-up fraction (9.7%, p= 0.001) than in the unprocessed fraction (27.0%), but not in the DGC fraction (27.8%, p= 0.098). The percentage of sperm cells with normal X or Y chromosomes was comparable in the three fractions. The percentage of sperm cells with abnormal chromatin structure significantly decreased after DGC (from 15.7% to 10.3%, p= 0.002). The swim-up method also tended to reduce the percentage of sperm cells with abnormal chromatin structure, but the difference was not significant (from 15.7% to 11.6%, p= 0.316). Conclusion: The swim-up method is superior for enriching genetically competent sperm.

키워드

참고문헌

  1. Li Z, Wang L, Cai J, Huang H. Correlation of sperm DNA damage with IVF and ICSI outcomes: a systematic review and meta-analysis. J Assist Reprod Genet 2006;23:367-76. https://doi.org/10.1007/s10815-006-9066-9
  2. Robinson L, Gallos ID, Conner SJ, Rajkhowa M, Miller D, Lewis S, et al. The effect of sperm DNA fragmentation on miscarriage rates: a systematic review and meta-analysis. Hum Reprod 2012;27:2908-17. https://doi.org/10.1093/humrep/des261
  3. Burrello N, Vicari E, Shin P, Agarwal A, De Palma A, Grazioso C, et al. Lower sperm aneuploidy frequency is associated with high pregnancy rates in ICSI programmes. Hum Reprod 2003;18:1371-6. https://doi.org/10.1093/humrep/deg299
  4. Nicopoullos JD, Gilling-Smith C, Almeida PA, Homa S, Nice L, Tempest H, et al. The role of sperm aneuploidy as a predictor of the success of intracytoplasmic sperm injection? Hum Reprod 2008;23:240-50.
  5. Henkel RR, Schill WB. Sperm preparation for ART. Reprod Biol Endocrinol 2003;1:108. https://doi.org/10.1186/1477-7827-1-108
  6. Enciso M, Iglesias M, Galan I, Sarasa J, Gosalvez A, Gosalvez J. The ability of sperm selection techniques to remove single- or double-strand DNA damage. Asian J Androl 2011;13:764-8. https://doi.org/10.1038/aja.2011.46
  7. Amiri I, Ghorbani M, Heshmati S. Comparison of the DNA fragmentation and the sperm parameters after processing by the density gradient and the swim up methods. J Clin Diagn Res 2012;6:1451-3.
  8. Jayaraman V, Upadhya D, Narayan PK, Adiga SK. Sperm processing by swim-up and density gradient is effective in elimination of sperm with DNA damage. J Assist Reprod Genet 2012;29:557-63. https://doi.org/10.1007/s10815-012-9742-x
  9. Xue X, Wang WS, Shi JZ, Zhang SL, Zhao WQ, Shi WH, et al. Efficacy of swim-up versus density gradient centrifugation in improving sperm deformity rate and DNA fragmentation index in semen samples from teratozoospermic patients. J Assist Reprod Genet 2014;31:1161-6. https://doi.org/10.1007/s10815-014-0287-z
  10. Ong TD, Xun L, Perreault SD, Robbins WA. Aneuploidy and chromosome breakage in swim-up versus unprocessed semen from twenty healthy men. J Androl 2002;23:270-7.
  11. Jakab A, Kovacs T, Zavaczki Z, Borsos A, Bray-Ward P, Ward D, et al. Efficacy of the swim-up method in eliminating sperm with diminished maturity and aneuploidy. Hum Reprod 2003;18:1481-8. https://doi.org/10.1093/humrep/deg288
  12. Kovanci E, Kovacs T, Moretti E, Vigue L, Bray-Ward P, Ward DC, et al. FISH assessment of aneuploidy frequencies in mature and immature human spermatozoa classified by the absence or presence of cytoplasmic retention. Hum Reprod 2001;16:1209-17. https://doi.org/10.1093/humrep/16.6.1209
  13. Kim SK, Jee BC, Kim SH. Histone methylation and acetylation in ejaculated human sperm: effects of swim-up and smoking. Fertil Steril 2015;103:1425-31. https://doi.org/10.1016/j.fertnstert.2015.03.007
  14. Tandara M, Bajic A, Tandara L, Bilic-Zulle L, Sunj M, Kozina V, et al. Sperm DNA integrity testing: big halo is a good predictor of embryo quality and pregnancy after conventional IVF. Andrology 2014;2:678-86. https://doi.org/10.1111/j.2047-2927.2014.00234.x
  15. Anifandis G, Bounartzi T, Messini CI, Dafopoulos K, Markandona R, Sotiriou S, et al. Sperm DNA fragmentation measured by Halosperm does not impact on embryo quality and ongoing pregnancy rates in IVF/ICSI treatments. Andrologia 2015;47:295-302. https://doi.org/10.1111/and.12259
  16. Pang MG, Kim YJ, Lee SH, Kim CK. The high incidence of meiotic errors increases with decreased sperm count in severe male factor infertilities. Hum Reprod 2005;20:1688-94. https://doi.org/10.1093/humrep/deh817
  17. Pang MG, You YA, Park YJ, Oh SA, Kim DS, Kim YJ. Numerical chromosome abnormalities are associated with sperm tail swelling patterns. Fertil Steril 2010;94:1012-20. https://doi.org/10.1016/j.fertnstert.2009.04.043
  18. Aitken RJ, De Iuliis GN, Finnie JM, Hedges A, McLachlan RI. Analysis of the relationships between oxidative stress, DNA damage and sperm vitality in a patient population: development of diagnostic criteria. Hum Reprod 2010;25:2415-26. https://doi.org/10.1093/humrep/deq214
  19. Fernandez JL, Muriel L, Goyanes V, Segrelles E, Gosalvez J, Enciso M, et al. Simple determination of human sperm DNA fragmentation with an improved sperm chromatin dispersion test. Fertil Steril 2005;84:833-42. https://doi.org/10.1016/j.fertnstert.2004.11.089
  20. Feijo CM, Esteves SC. Diagnostic accuracy of sperm chromatin dispersion test to evaluate sperm deoxyribonucleic acid damage in men with unexplained infertility. Fertil Steril 2014;101:58-63.e3. https://doi.org/10.1016/j.fertnstert.2013.09.002
  21. Ribas-Maynou J, Garcia-Peiro A, Fernandez-Encinas A, Abad C, Amengual MJ, Prada E, et al. Comprehensive analysis of sperm DNA fragmentation by five different assays: TUNEL assay, SCSA, SCD test and alkaline and neutral Comet assay. Andrology 2013;1:715-22. https://doi.org/10.1111/j.2047-2927.2013.00111.x
  22. Marchesi DE, Biederman H, Ferrara S, Hershlag A, Feng HL. The effect of semen processing on sperm DNA integrity: comparison of two techniques using the novel toluidine blue assay. Eur J Obstet Gynecol Reprod Biol 2010;151:176-80. https://doi.org/10.1016/j.ejogrb.2010.05.003
  23. Brahem S, Letaief K, Ben Ali H, Saad A, Mehdi M. Efficacy of the density gradient centrifugation method in eliminating sperm with aneuploidy. Andrologia 2013;45:158-62. https://doi.org/10.1111/j.1439-0272.2012.01327.x
  24. Piomboni P, Stendardi A, Gambera L. Chromosomal aberrations and aneuploidies of spermatozoa. Adv Exp Med Biol 2014;791:27-52.
  25. Muriel L, Goyanes V, Segrelles E, Gosalvez J, Alvarez JG, Fernandez JL. Increased aneuploidy rate in sperm with fragmented DNA as determined by the sperm chromatin dispersion (SCD) test and FISH analysis. J Androl 2007;28:38-49.
  26. Bronet F, Martinez E, Gaytan M, Linan A, Cernuda D, Ariza M, et al. Sperm DNA fragmentation index does not correlate with the sperm or embryo aneuploidy rate in recurrent miscarriage or implantation failure patients. Hum Reprod 2012;27:1922-9. https://doi.org/10.1093/humrep/des148
  27. Vendrell X, Ferrer M, Garcia-Mengual E, Munoz P, Trivino JC, Calatayud C, et al. Correlation between aneuploidy, apoptotic markers and DNA fragmentation in spermatozoa from normozoospermic patients. Reprod Biomed Online 2014;28:492-502. https://doi.org/10.1016/j.rbmo.2013.12.001

피인용 문헌

  1. Sperm selection with density gradient centrifugation and swim up: effect on DNA fragmentation in viable spermatozoa vol.9, pp.None, 2019, https://doi.org/10.1038/s41598-019-43981-2
  2. Effect of Sperm DNA Fragmentation on Embryo Quality in Normal Responder Women in In Vitro Fertilization and Intracytoplasmic Sperm Injection vol.60, pp.5, 2019, https://doi.org/10.3349/ymj.2019.60.5.461
  3. Evaluation of normal morphology, DNA fragmentation, and hyaluronic acid binding ability of human spermatozoa after using four different commercial media for density gradient centrifugation vol.46, pp.1, 2017, https://doi.org/10.5653/cerm.2019.46.1.8
  4. Mammalian spermatozoa and cumulus cells bind to a 3D model generated by recombinant zona pellucida protein-coated beads vol.9, pp.1, 2019, https://doi.org/10.1038/s41598-019-54501-7
  5. The Author Reply: Effect of Sperm DNA Fragmentation on Embryo Quality in Normal Responder Women in IVF and ICSI vol.61, pp.11, 2020, https://doi.org/10.3349/ymj.2020.61.11.988
  6. Best laboratory practices and therapeutic interventions to reduce sperm DNA damage vol.53, pp.2, 2021, https://doi.org/10.1111/and.13736
  7. Differential impact of four sperm preparation techniques on sperm motility, morphology, DNA fragmentation, acrosome status, oxidative stress, and mitochondrial activity: A prospective study vol.9, pp.5, 2017, https://doi.org/10.1111/andr.13038