Clinical and Experimental Reproductive Medicine

  • 제44권4호
  • /
  • Pages.193-200
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  • 2017
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  • 2233-8233(pISSN)
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  • 2233-8241(eISSN)
대한생식의학회 (The Korean Society for Reproductive Medicine)
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Effects of laser-assisted hatching and exposure time to vitrification solution on mouse embryo development

  • Kim, Hye Jin (Department of Obstetrics and Gynecology, Chungnam National University Hospital) ;
  • Park, Sung Baek (Mirae i Infertility Clinic, Mirae Ladies Hospital) ;
  • Yang, Jung Bo (Department of Obstetrics and Gynecology, Chungnam National University Hospital) ;
  • Choi, Young Bae (Mirae i Infertility Clinic, Mirae Ladies Hospital) ;
  • Lee, Ki Hwan (Department of Obstetrics and Gynecology, Chungnam National University Hospital)
  • 투고 : 2017.07.13
  • 심사 : 2017.10.18
  • 발행 : 2017.12.31
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초록

Objective: This study was conducted to investigate the efficacy of laser-assisted hatching (LAH) and various vitrification times for embryonic development and blastocyst cell numbers. Methods: First, 2-cell and 8-cell embryos were collected by flushing out the oviducts. In the control groups, they were vitrified for 8 or 10 minutes without LAH. The LAH groups underwent quarter laser zona thinning-assisted hatching before vitrification (4, 6, and 8 minutes or 4, 7, and 10 minutes, respectively). After incubation, double-immunofluorescence staining was performed. Results: The hatched blastocyst rate 72 hours after the 2-cell embryos were thawed was significantly higher in the 2LAH-ES8 group (33.3%) than in the other groups (p< 0.05). In the control-8 group ($22.1{\pm}4.6$), the cell number of the inner cell mass was higher than in the LAH groups (p< 0.05). The number of trophectoderm cells was higher in the 2LAH-ES6 group ($92.8{\pm}8.9$) than in the others (p< 0.05). The hatched blastocyst rate 48 hours after the 8-cell embryos were thawed was higher in the 8LAH-ES4 group (45.5%) than in the other groups, but not significantly. The inner cell mass cell number was highest in the 8LAH-ES7 group ($19.5{\pm}5.1$, p< 0.05). The number of trophectoderm cells was higher in the 8LAH-ES10 group ($73.2{\pm}12.1$) than in the other groups, but without statistical significance. Conclusion: When LAH was performed, 2-cell embryos with large blastomeres had a lower hatched blastocyst rate when the exposure to vitrification solution was shorter. Conversely, 8-cell embryos with small blastomere had a higher hatched blastocyst rate when the exposure to vitrification solution was shorter.

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참고문헌

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