Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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Antioxidant and Anti-inflammatory Effects of Dioscorea japonica and Chenopodium album

참마와 명아주의 항산화 및 항염증 효과

  • Kim, Shin Ae (Department of Chemistry, College of Natural science, Kunsan Natural University) ;
  • Choi, Soo Cheol (Department of Chemistry, College of Natural science, Kunsan Natural University) ;
  • Youn, Young Han (Department of Beauty Care, Sohae College) ;
  • Ko, Chang In (Department of Information Telecommunication Engineering, college of Engineering, Kunsan Natural University) ;
  • Ha, Young Soon (C & S Co., Ltd.) ;
  • Lee, In-Ah (Department of Chemistry, College of Natural science, Kunsan Natural University)
  • 김신애 (군산대학교 자연과학대학 화학과) ;
  • 최수철 (군산대학교 자연과학대학 화학과) ;
  • 윤영한 (서해대학교 뷰티케어과) ;
  • 고창인 (군산대학교 공과대학 정보통신학과) ;
  • 하영순 ((주)씨엔에스) ;
  • 이인아 (군산대학교 자연과학대학 화학과)
  • Received : 2017.11.06
  • Accepted : 2017.12.22
  • Published : 2017.12.30
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Abstract

In this study, free radical scavenging activity and enzyme-linked immunosorbent assay (ELISA) experiments were carried out using Dioscorea japonica (D. japonica) and Chenopodium album (C. album) extracts to evaluate their anti-oxidative and anti-inflammatory effects. In the free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activity, $EC_{50}$ of D. japonica and C. album were measured as 2.386 and 0.524 mg/mL, respectively. The free radical scavenging activity of the mixed sample of D. japonica and C. album was the highest when the D. japonica and C. album ratio was 2 : 1. The IL-6 and $TNF-{\alpha}$ ELISA assay showed that IL-6 in mouse spleen cells treated 1 mg/mL of samples, D. japonica and C. album decreased the production of IL-6 concentration by 27.17%, 72.30%. In the case of $TNF-{\alpha}$, D. japonica and C. album decreased 61.97% and 77.85% of $TNF-{\alpha}$ production, respectively. Through these results, we confirmed that D. japonica and C. album have antioxidant and anti - inflammatory effects and could be applied to natural medicine cosmetic having anti - inflammatory effects.

본 연구에서는 참마와 명아주의 항산화 및 항염증 효능을 평가하기 위해 참마와 명아주 에탄올 추출물을 이용하여 free radical 소거활성, enzyme-linked immunosorbent assay (ELISA) 실험을 수행하였다. 참마와 명아주 추출물의 free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) 소거활성($EC_{50}$)에서 각각 2.386, 0.524 mg/mL로 측정되었다. 또한 참마와 명아주 추출물의 혼합 시료의 free radical 소거활성은 참마 추출물:명아주 추출물 비율이 2:1일 때 가장 뛰어난 것으로 나타났다. IL-6와 $TNF-{\alpha}$의 ELISA 실험을 통해 항염증효능을 평가한 결과, 마우스 비장세포에서 IL-6의 경우 1 mg/mL 농도에서 참마 추출물은 대조군과 대비하여 27.17% IL-6 생성을 감소시켰으며, 명아주 추출물은 72.30%의 감소를 나타내었다. $TNF-{\alpha}$의 경우 참마 추출물은 61.97%, 명아주 추출물은 77.85%로 유의성 있는 $TNF-{\alpha}$생성 감소 효능을 나타내었다. 이 결과들을 통하여 참마와 명아주 추출물은 항산화, 항염증 효능을 가지고 있으며, 이를 활용하여 항염증에 효과가 있는 천연물 제제에 응용 가능성이 있음을 확인하였다.

Keywords

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