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Lack of allozyme variation in the two carnivorous, terrestrial herbs Utricularia bifida and Utricularia caerulea (Lentibulariaceae) co-occurring on wetlands in South Korea: Inference of population history

한반도 남부 지방 습지에 같이 자생하는 식충 육상 초본 2종 땅귀개 및 이삭귀개 (통발과)의 알로자임 변이의 결여: 집단의 역사 추론

  • Chung, Mi Yoon (Division of Life Science and the Research Institute of Natural Science, Gyeongsang National University) ;
  • Lopez-Pujol, Jordi (BioC-GReB, Botanic Institute of Barcelona (IBB-CSIC-ICUB)) ;
  • Chung, Myong Gi (Division of Life Science and the Research Institute of Natural Science, Gyeongsang National University)
  • 정미윤 (경상대학교 생명과학부 및 기초과학연구소) ;
  • ;
  • 정명기 (경상대학교 생명과학부 및 기초과학연구소)
  • Received : 2017.12.04
  • Accepted : 2017.12.22
  • Published : 2017.12.30

Abstract

In central and southern Korea, the two small insectivorous, terrestrial herbs, Utricularia bifida and U. caerulea, often co-occur at wet locations (or in wetlands). The Korean Peninsula (with central China and northern Japan) constitutes the northern edge of their distribution, as their main range is subtropical and tropical Asia. The Korean populations of both species are very likely of post-glacial origin, given that warm-temperate vegetation was absent from the Korean Peninsula during the Last Glacial Maximum. Two hypotheses of the post-glacial colonization of the peninsula can be formulated; first, if current populations were founded by propagules coming from a single ancestral population (i.e., a single refugium), we would expect low levels of genetic diversity. Alternatively, if contemporary Korean populations originated from multiple sources (multiple refugia), we would expect high levels of genetic variation. To test which is more likely, we surveyed the degree of allozyme variation at 20 loci in ten populations for each of the two species from southern Korea. We found no allozyme variation within each species. However, their aquatic congener U. australis exhibited allozyme polymorphism across Japan (four polymorphic loci at three enzyme systems). We suggest that southern Korean populations of Utricularia bifida and U. caerulea were established by a single introduction event from a genetically depauperate ancestral population.

한반도 중부 및 남부에 작은 육상성 식충식물(땅속줄기에서 벌레잡이주머니 존재)인 땅귀개와 이삭 귀개가 종종 습한 장소(또는 습지 내)에서 같이 서식한다. 이들 2종은 아열대 및 열대 아시아의 주요 산지이기 때문에 한반도는 중국 중부 및 일본 북부 지역과 더불어 분포의 북방한계이다. 최후의 빙하기 최대 기간동안 따뜻한 온대 식물이 한반도에 없다는 점을 감안할 때 두 종의 한반도 집단은 빙하기 이후에 기원했을 가능성이 매우 높다. 한반도에서 빙하기 이후 정착에 대한 두 가설을 제시할 수 있다. 첫째로, 현재 집단이 단일 조상 개체군(즉, 하나의 피난처)으로부터 유래된 자손에 의해 형성되었다면, 우리는 낮은 수준의 유전적 다양성을 기대할 것이다. 반면에, 현재 한반도 집단이 여러 집단(몇 곳의 피난처)에서 유래되었다면, 우리는 높은 수준의 유전적 변이를 기대할 수 있다. 어떤 가설이 더 타당한지를 검증하기 위해, 저자들은 한반도 남부지방 10곳 지역을 대상으로 알로자임 변이를 조사하였다. 저자들은 각 종 내에서 알로자임 변이가 없음을 발견했다. 그러나, 기존 연구된 그들의 수생 동속종인 Utricularia australis는 일본 전 지역을 대상으로 채집된 자료에서 알로자임 다형성을 보였다(3개의 효소 시스템에서 4개의 다형성 좌위가 보고됨). 저자들은 땅귀개와 이삭귀개가 남한으로 각각 개체군이 유전적 다양도가 낮은 조상집단에서 한번 도입되었을 가능성을 제안한다.

Keywords

References

  1. Araki, S. 2000. Isozyme differentiation between two infraspecies taxa of Utricularia australis R. Br. (Lentibulariaceae) in Japan. Acta Phytotaxonomica et Geobotanica 51: 31-36.
  2. Araki, S. and Y. Kadono. 2003. Restricted seed contribution and clonal dominance in a free-floating aquatic plant Utricularia australis R. Br. in southwestern Japan. Ecological Research 18: 599-609. https://doi.org/10.1046/j.1440-1703.2003.00581.x
  3. Cheliak, W. M. and J. A. Pitel. 1984. Technique for Starch Gel Electrophoresis of Enzyme from Forest Tree Species. Information Report PI-X-42. Petawawa National Forestry Institute, Chalk River, ON, 49 pp.
  4. Chung, M. G. 1999. Notes on allozyme variation in Lycoris radiata (Amaryllidaceae) from Korea. Botanical Bulletin of Academia Sinica 40: 227-230.
  5. Chung, J. M., K. W. Park, C.-S. Park, S.-H. Lee, M. G. Chung and M. Y. Chung. 2009. Contrasting levels of genetic diversity between the historically rare orchid Cypripedium japonicum and the historically common orchid Cypripedium macranthos in South Korea. Botanical Journal of the Linnean Society 160: 119-129. https://doi.org/10.1111/j.1095-8339.2009.00965.x
  6. Chung, M. Y. 2009. Lack of allozyme diversity in populations of the rare, endangered terrestrial orchids Tipularia japonica and Epipactis papillosa in Korea. Plant Systematics and Evolution 278: 203-209. https://doi.org/10.1007/s00606-008-0140-x
  7. Chung, M. Y., J. Lopez-Pujol and M. G. Chung. 2013. Population history of the two carnivorous plants Drosera peltata var. nipponica and Drosera rotundifolia (Droseraceae) in Korea. American Journal of Botany 100: 2231-2239. https://doi.org/10.3732/ajb.1200486
  8. Chung, M. Y., J. Lopez-Pujol and M. G. Chung. 2016. Notes on genetic variation in Sedum sarmentosum (Crassulaceae): Implications for the origin of southern Korean populations. Korean Journal of Plant Taxonomy 46: 371-377. https://doi.org/10.11110/kjpt.2016.46.4.371
  9. Chung, M. Y., J. Lopez-Pujol and M. G. Chung. 2017a. The role of the Baekudaegan (Korean Peninsula) as a major glacial refugium for plant species: A priority for conservation. Biological Conservation 206: 236-248. https://doi.org/10.1016/j.biocon.2016.11.040
  10. Chung, M. Y., J. D. Nason and M. G. Chung. 2005. Patterns of hybridization and population genetic structure in the terrestrial orchids Liparis kumokiri and Liparis makinoana (Orchidaceae) in sympatric populations. Molecular Ecology 14: 4389-4402. https://doi.org/10.1111/j.1365-294X.2005.02738.x
  11. Chung, M. Y., C.-W. Park, E. R. Myers and M. G. Chung. 2007. Contrasting levels of genetic diversity between the common, self-compatible Liparis kumokiri and rare, self-incompatible Liparis makinoana (Orchidaceae) in South Korea. Botanical Journal of the Linnean Society 153: 41-48. https://doi.org/10.1111/j.1095-8339.2007.00581.x
  12. Chung, M. Y., J. Lopez-Pujol, M. Maki, K.-J. Kim, J. M. Chung, B.-Y. Sun and M. G. Chung. 2012. Genetic diversity in the common terrestrial orchid Oreorchis patens and its rare congener Oreorchis coreana: inference of species evolutionary history and implications for conservation. Journal of Heredity 103: 692-702. https://doi.org/10.1093/jhered/ess032
  13. Chung, M. Y., J. Lopez-Pujol, S. Son, G. U. Suh, T. Yukawa and M. G. Chung. 2017b. Patterns of genetic diversity in rare and common orchids focusing on the Korean Peninsula: Implications for conservation. The Botanical Review. Advanced online publication. https://doi.org/10.1007/s12229-017-9190-5.
  14. Chung, M. Y., H. T. Q. Le, S. Son and M. G. Chung. In press. Genetic diversity of the extremely rare Habenaria dentata and the rare Habenaria linearifolia (Orchidaceae) in South Korea: implications for population history and conservation. Plant Ecology and Evolution.
  15. Clayton, J. W. and D. N. Tretiak. 1972. Amine-citrate buffers for pH control in starch gel electrophoresis. Journal of the Fisheries Research Board of Canada 29: 1169-1172. https://doi.org/10.1139/f72-172
  16. Clivati, D., G. D. Cordeiro, B. J. Plachno and V. F. O. de Miranda. 2013. Reproductive biology and pollination of Utricularia reniformis A. St.-Hil. (Lentibulariaceae). Plant Biology 16: 677-682.
  17. Denny, P. 1994. Biodiversity and wetlands. Wetlands Ecology and Management 3: 55-61.
  18. Eriksson, O. and K. Kainulainen. 2011. The evolutionary ecology of dust seeds. Perspectives in Plant Ecology, Evolution and Systematics 13: 73-87. https://doi.org/10.1016/j.ppees.2011.02.002
  19. Godt, M. J. W. and J. L. Hamrick. 2001. Genetic diversity in rare southeastern plants. Natural Areas Journal 21: 61-70.
  20. Gottlieb, L. D. 1982. Conservation and duplication of isozymes in plants. Science 216: 373-380. https://doi.org/10.1126/science.216.4544.373
  21. Gray, A. 1996. Genetic diversity and its conservation in natural populations of plants. Biodiversity Letters 3: 71-80. https://doi.org/10.2307/2999720
  22. Halls, A. J. 1997. Wetlands, Biodiversity and the Ramsar Convention: The Role of the Convention on Wetlands in the Conservation and Wise Use of Biodiversity. Ramsar Convention Bureau, Gland, Switzerland, 168 pp.
  23. Haufler, C. H. 1985. Enzyme variability and modes of evolution in Bommeria (Pteridaceae). Systematic Botany 10: 92-104. https://doi.org/10.2307/2418438
  24. Hewitt, G. 2000. The genetic legacy of the Quaternary ice ages. Nature 405: 907−913. https://doi.org/10.1038/35016000
  25. Hobbhahn, N., H. Kuchmeister and S. Porembski. 2006. Pollination biology of mass flowering terrestrial Utricularia species (Lentibulariaceae) in the Indian Western Ghats. Plant Biology 8: 791-804. https://doi.org/10.1055/s-2006-924566
  26. Hu, F. S., A. Hampe and R. J. Petit. 2009. Paleoecology meets genetics: deciphering past vegetational dynamics. Frontiers in Ecology and the Environment 7: 371−379. https://doi.org/10.1890/070160
  27. Kao, S. J., C.-R. Wu, Y.-C. Hsin and M. Dai. 2006. Effects of sea level change on the upstream Kuroshio Current through the Okinawa Trough. Geophysical Research Letters 33: L16604. https://doi.org/10.1029/2006GL026822
  28. KNA (Korea National Arboretum). 2012. Rare Plants in Korea. Korea National Arboretum, Pocheon, 412 pp. (in Korean)
  29. Lee, M. 2007. Present status and conservation strategy of the mountainous wetlands in Ulsan city and Gyeongsangnamdo Province. Master's Thesis in Biology Education, Gyeongsang National University, Jinju, South Korea, 51 pp. (in Korean with English summary)
  30. Li, Z. and M. R. Cheek. 2011. Utricularia Linneaeus. In Flora of China, Vol. 19. Cucurbitaceae through Valerianaceae, with Annonaceae and Berberidaceae. Wu, Z.-Y., P. H. Raven and D. Y. Hong (eds.), Science Press, Beijing and Missouri Botanical Garden Press, St. Louis, MO. Pp. 481-491.
  31. Losos, J. B. 2008. Phylogenetic niche conservatism, phylogenetic signal and the relationship between phylogenetic relatedness and ecological similarity among species. Ecology Letters 11: 995-1003. https://doi.org/10.1111/j.1461-0248.2008.01229.x
  32. Menezes, C. G., E. C. Gasparino, P. C. Baleeiro and V. F. O. de Miranda. 2014. Seed morphology of bladderworts: A survey on Utricularia sect. Foliosa and sect. Psyllosperma (Lentibulariaceae) with taxonomic implications. Phytotaxa 167: 173-182. https://doi.org/10.11646/phytotaxa.167.2.3
  33. Mitton, J. B., Y. B. Linhart, K. B. Sturgeon and J. L. Hamrick. 1979. Allozyme polymorphisms detected in mature needle tissue of ponderosa pine. Journal of Heredity 70: 86-89. https://doi.org/10.1093/oxfordjournals.jhered.a109220
  34. MOE (Ministry of Environment, Republic of Korea). 2014. Korean Red List of Threatened Species. 2nd ed. National Institute of Biological Resources, Incheon, 242 pp.
  35. Morales, E. 2000. Estimating phylogenetic inertia in Tithonia (Asteraceae): a comparative approach. Evolution 54: 475-484. https://doi.org/10.1111/j.0014-3820.2000.tb00050.x
  36. Nei, M. 1973. Analysis of gene diversity in subdivided populations. Proceedings of National Academy of Sciences of the United States of America 70: 3321-3323.
  37. Nei, M. 1977. F-statistics and analysis of gene diversity in subdivided populations. Annals of Human Genetics 41: 225-233. https://doi.org/10.1111/j.1469-1809.1977.tb01918.x
  38. Petit, R. J., I. Aguinagalde, J.-L. de Beaulieu, C. Bittkau, S. Brewer, R. Cheddadi, R. Ennos, S. Fineschi, D. Grivet, M. Lascoux, A. Mohanty, G. Muller-Starck, B. Demesure-Musch, A. Palme, J. P. Martin, S. Rendell and G. G. Vendramin. 2003. Glacial refugia: hotspots but not melting pots of genetic diversity. Science 300: 1563-1565. https://doi.org/10.1126/science.1083264
  39. Rull, V. 2009. Microrefugia. Journal of Biogeography 36: 481-484. https://doi.org/10.1111/j.1365-2699.2008.02023.x
  40. Soltis, D. E., C. H. Haufler, D. C. Darrow and G. J. Gastony. 1983. Starch gel electrophoresis of ferns: a compilation of grinding buffers, gel and electrode buffers, and staining schedules. American Fern Journal 73: 9-27. https://doi.org/10.2307/1546611
  41. Taylor, P. 1989. The Genus Utricularia: A Taxonomic Monograph. The Royal Botanic Gardens, Kew, London, 724 pp.
  42. Weeden, N. F. and J. F. Wendel. 1989. Genetics of plant isozymes. In Isozymes in Plant Biology. Soltis, D. E. and P. S. Soltis (eds.), Dioscorides Press, Portland. Pp. 46-72.

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