Applied Microscopy

  • 제47권4호
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  • Pages.215-217
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  • 2017
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  • 2287-5123(pISSN)
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  • 2287-4445(eISSN)
한국현미경학회 (Korean Society of Microscopy)
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Cryo-Transmission Electron Microscopy in Korean Society of Microscopy

  • Han, Sung Sik (School of Life Sciences and Biotechnology, Korea University)
  • 투고 : 2017.11.29
  • 심사 : 2017.12.05
  • 발행 : 2017.12.30
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초록

Dr. Jacques Dubochet, Dr. Joachim Frank, and Dr. Richard Henderson received the 2017 Nobel Prize for Chemistry for their efforts to develop effective ways to obtain high-resolution three-dimensional images of biomolecules using cryo-electron microscopy. Congratulations to the Nobel Prize in the field of electron microscopy, I will explain the scientific contributions of the three winners and introduce the role of cryo-electron microscopy (including cryo technology) in biology.

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참고문헌

  1. Cavalier A, Spehner D, and Humbel B M (2008) Handbook of Cryo-Preparation Methods for Electron Microscopy (CRC Press, Boca Raton).
  2. Dubochet J, Adrian M, Chang J J, Homo J C, Lepault J, McDowall A W, and Schultz P (1988) Cryo-electron microscopy of vitrified specimens. Quart. Rev. Biophys. 21, 129-228. https://doi.org/10.1017/S0033583500004297
  3. Dubochet J, Lepault J, Freeman R, Berriman J A, and Homo J C (1982) Electron microscopy of frozen water and aqueous solutions. J. Microsc. 128, 219-237. https://doi.org/10.1111/j.1365-2818.1982.tb04625.x
  4. Dubochet J and McDowall A W (1981) Vitrification of pure water for electron microscopy. J. Microsc. 124, 3-4.
  5. Frank J (1975) Averaging of low exposure electron micrographs of nonperiodic objects. Ultramicroscopy 1, 159-162. https://doi.org/10.1016/S0304-3991(75)80020-9
  6. Frank J (1996) In Three-Dimensional Electron Microscopy of Macromolecular Assemblies, pp. xv-xvii (Academic Press, Burlington).
  7. Frank J (2005) Electron Tomography, Methods for Three-Dimensional Visualization of Structure in the Cell (Springer, New York).
  8. Frank J (2006) Three-Dimensional Electron Microscopy of Macromolecular Assemblies: Visualization of Biological Molecules in Their Native State (Oxford University Press, Oxford).
  9. Frank J, Penczek P, Grassucci R, and Srivastava S (1991) Three-dimensional reconstruction of the 70S Escherichia coli ribosome in ice: the distribution of ribosomal RNA. J. Cell Biol. 115, 597-605. https://doi.org/10.1083/jcb.115.3.597
  10. Henderson R and Unwin P N T (1975) Three-dimensional model of purple membrane obtained by electron microscopy. Nature 257, 28. https://doi.org/10.1038/257028a0