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Development of Lentil Cheonggukjang Fermented by Bacillus subtilis Isolated from Traditional Soy Sauce

재래간장으로부터 분리한 Bacillus subtilis를 이용하여 제조한 렌틸콩 청국장의 개발

  • Lee, Yeon Jeong (Department of Food and Nutrition, Kyung Hee University) ;
  • Kim, Na Young (Department of Hotel Culinary Art, Songho College) ;
  • Kim, Up Sik (Department of Hotel Culinary Art, Yeonsung University) ;
  • Han, Myung Joo (Department of Food and Nutrition, Kyung Hee University)
  • 이연정 (경희대학교 식품영양학과) ;
  • 김나영 (송호대학교 호텔외식조리학과) ;
  • 김업식 (연성대학교 호텔조리과) ;
  • 한명주 (경희대학교 식품영양학과)
  • Received : 2017.08.16
  • Accepted : 2017.12.20
  • Published : 2017.12.31

Abstract

This study was conducted to screen for superior strains for preparation of Cheonggukjang and identify the optimal fermentation time based on fermentation of lentils by various Bacillus subtilis strains. Bacillus count was significantly high at 48 hrs of fermentation (9.22-9.51 log CFU/g). In addition, the pH was significantly high in the range of 7.60-7.92 at 48 hrs fermentation, and the reducing sugar content was significantly high (0.89-1.45). The amino-type nitrogen content and ammonia-type nitrogen content were significantly increased with fermentation time. Additionally, the amylase activity was significantly high (3.05-4.22) at 48 hrs of fermentation, as was the protease activity (4.80-5.63). Bacillus subtilis S3 (5.63), S4 (5.60) were higher than S1 (4.80), S2 (4.92). The viscous substances and DPPH radical scavenging activity increased at 48 hrs, then decreased. Sensory evaluation revealed that lentil Cheonggukjang fermented by S3 showed the highest scores in color, smell, savory taste, sweet taste, viscous substance and overall acceptability. Therefore, 48 hrs of fermentation should be suitable for lentil Cheonggukjang fermented by Bacillus subtilis S3.

Keywords

References

  1. Ahn BS, Lee CH. 2003. Changes in microbial and chemical composition and sensory characteristics of fermented soybean paste, Chungkukjang, by high dose gamma irradiation. Korean J. Food Sci. Technol., 35(2):166-172
  2. Ahn YS, Kim YS, Shin DH. 2006. Isolation, identification, and fermentation characteristics of Bacillus sp. with high protease activity from traditional Cheonggukjang. Korean J. Food Sci. Technol., 38(1):82-87
  3. Amarakoon D, McPhee K, Thavarajah P. 2012. Iron-, zinc-, and magnesium-rich field peas (Pisum sativum L.) with naturally low phytic acid: A potential food-based solution to global micronutrient malnutrition. J. Food Composition Analysis, 27(1):8-13 https://doi.org/10.1016/j.jfca.2012.05.007
  4. Anson ML. 1938. The estimation of pepsin, trypsin, papain, and cathepsin with hemoglobin. J. General Physiology, 22(1): 79-89 https://doi.org/10.1085/jgp.22.1.79
  5. Baek JE, Choi YH, Song J, Yun HT, Choi HS, Park SY. 2014. Physicochemical properties of Cheonggukjang with fermentation period for a variety of Soybean cultivars. Korean J. Food Nutr., 27(4):742-750 https://doi.org/10.9799/ksfan.2014.27.4.742
  6. Blois MS. 1958. Antioxidant determinations by the use of a stable free radical. Nature, 181:1199-1200 https://doi.org/10.1038/1811199a0
  7. Chae SG, Ryu ID, Ma SJ, Bang KW, Oh MH, Oh SH. 2006. Food analysis standards. Jigumunhwasa, Seoul, pp 219-228
  8. Choi YJ. 2016. Isolation of Bacillus subtilis from traditional soy sauce and quality characteristics of Asparagus Cheonggukdoenjang. Masters degree thesis, Kyunghee University, Seoul, Korea, pp 45-46
  9. Chung YC, Chang CT, Chao WW, Lin CF, Chou ST. 2002. Antioxidative activity and safety of the 50 ethanolic extract from red bean fermented by Bacillus subtilis IMRNK1. J. Agri. Food Chem., 50(8): 2454-2458 https://doi.org/10.1021/jf011369q
  10. Hwang H, Lee NK, Cho IJ, Hahm YT, Kwon KO, Kim BY. 2008. Selection of microorganisms and optimization of manufacture process for Cheonggukjang. Korean J. Food Sci. Technol., 40(4):40-411
  11. Hwang SH, Chung HS, Kim S, Youn K. 2004. Effect of Glycyrrhizia uralensis extract addition on the quality of cheonggukjang. J. East Asian Soc. Dietary Life, 14:571-575
  12. In JP. 2004. Effect of yucca (Yucca shidigera) extract on quality characteristics of Chungkookjang using Bacillus subtilis p01. J. Korean Soc. Appl. Biol. Chem., 47:176-181
  13. Jiang CK. 2007. Quality characteristics, antioxidant and antitumor effects of cheonggukjang with Phellinus linteus extract. Master degree thesis, Konkuk University, Seoul, Korea, pp 30-35
  14. Joo HK, Kim ND, Yoon KS. 1989. Changes of enzymatic activities during the fermentation of soybean-soypaste by Aspergillus spp. J. Korean Agri. Chem. Soc., 24(3):295-302
  15. Jung JB, Choi SK, Jeong DY, Kim YS, Kim YS. 2012. Effects of germination time of soybeans on quality characteristics of cheonggukjang fermented with an isolated bacterial strain. Korean J. Food Sci. Technol.., 44(1):69-75 https://doi.org/10.9721/KJFST.2012.44.1.069
  16. Kang HM, Lee SH, Park NY. 2013. Fermentation and quality characteristics of Cheonggukjang prepared with sea mustard. J. East Asian Soc. Dietary Life, 23(6):810-817
  17. Kim KJ, Ryu MK, Kim SS. 1982. Chungkook-jang koji fermentation with rice straw. Korean J. Food Sci. Technol., 14(4):301-308
  18. Kim KM, Kim HR, Park HJ. 2006. The Quality characteristics of Chunggujang prepared by Bacillus Subtilis NRLSI IV on the different inoculum levels and fermentation times. The Korean J. Community Living Sci., 17(3):123-131
  19. Kwak CS, Lee MS, Park SC. 2007. Higher antioxidant properties of Chungkookjang, a fermented soybean paste, may be due to increased aglycone and malonylglycoside isoflavone during fermentation. Nutrition Research, 27(11):719-727 https://doi.org/10.1016/j.nutres.2007.09.004
  20. Kweon MH, Hwang HJ, Sung HC. 2001. Identification and antioxidant activity of novel chlorogenic acid derivatives from bamboo (Phyllostachys edulis). J. Agri. Food Chem., 49(10):4646-4655 https://doi.org/10.1021/jf010514x
  21. Lee HJ, Park HO, Jang JS, Kim SS, Han CK, Oh JB, Do WY. 2011. Antioxidant activity and quality characteristics of American cookies prepared with job's tears (Coix lachryma-jobi L.) Chungkukjang powder and wheat bran powder. Korean J. Food Nutr., 24(1):85-93 https://doi.org/10.9799/ksfan.2011.24.1.085
  22. Lee MY, Park SY, Jung KO, Park KY, Kim SD. 2005. Quality and functional characteristics of Chungkukjang prepared with various Bacillus sp. isolated from traditional Chungkukjang. J. Food Sci. 70(4):191-196
  23. Lee NR, Go TH, Park SB, Lee SM, Hwang DY, Kim DS, Park GT, Hong JS. 2013. Fermentation of germinated- and nongerminated-yellow soybean Chungkookjang using Bacillus subtilis. Korean J. Microbiol. Biotechnol., 41(2): 160-167 https://doi.org/10.4014/kjmb.1211.11006
  24. Lee SH, Han NR, Lee SH. 2015. Effect of lentil bean on fermentation and quality characteristics of Cheonggukjang. Korean J. Food Sci. Technol., 13(1):65-71
  25. Lee YL, Kim SH, Choung NH, Yim MH. 1992. A study on the production of viscous substance during the Chungkookjang fermentation. J. Korean Soc. Appl. Biol. Chem., 35(3):202-209
  26. Mann SY, Kim EA, Lee GY, Kim RU, Hwang DY, Son HJ, Lee BW, Lee CY, Kim DS. 2013. Characteristics of Chungkookjang produced by Bacillus subtillus MC31. J. Life Sci., 23(4):560-568 https://doi.org/10.5352/JLS.2013.23.4.560
  27. Miller GL. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chem., 31(3): 426-428 https://doi.org/10.1021/ac60147a030
  28. Min MJ, Shin HJ. 2015. Chemical Composition and Nutritional Characteristics of Lentils (Lens culinaris), and Their Application in the Food Industry: A Review, Korean J. Food Sci. Technol., 47(3):273-280 https://doi.org/10.9721/KJFST.2015.47.3.273
  29. Natella F, Nardini M, Di Felice M, Scaccini C. 1999. Benzoic and cinnamic acid derivatives as antioxidants: structureactivity relation. J. Agri. Food Chem., 47(4):1453-1459 https://doi.org/10.1021/jf980737w
  30. Park HY, Cho EJ. 2008. Radical scavenging effects and physicochemical properties of Seolitae Chungkukjang added with green tea. J. Korean Soc. Food Sci. Nutr., 37(4):401-404 https://doi.org/10.3746/jkfn.2008.37.4.401
  31. Shon MY. 1999. Physicochemical properties and biological activities of Chungkugjang produced from Korean black bean. Ph.D. Dissertation. Gyeongsang National University, Gyeongsangnamdo, Korea, pp 48-54
  32. Song YJ. 2015. Quality Characteristics of Cheonggukjang Fermented by Bacillus spp. Masters degree thesis, Chonbuk National University, Jeollabukdo, Korea, pp 27-34
  33. Sung NJ, Ji Y, Chung SY. 1984. Changes in nitrogenous compounds of soybean during chungkookjang koji fermentation. J. Korean Soc. Food Sci. Nutr. 13(3):275-284
  34. Tamang JP. 2015. Health benefits of fermented foods and beverages. CRC Press. U.K., pp 231-245
  35. Torino MI, Limon RI, Martinez-Villaluenga C, Makinen S, Pihlanto A, Vidal-Valverde C, Frias J. 2013. Antioxidant and antihypertensive properties of liquid and solid state fermented lentils. Food Chem., 136(2):1030-1037 https://doi.org/10.1016/j.foodchem.2012.09.015
  36. Woo SM, Kwon JH, Jeong YJ. 2006. Selection and fermentation characteristics of Cheonggukjang strains. Korean J. Food Preservation, 13(1):77-82
  37. Yang EI, Kim YS. 2013. Physiological Properties of Viscous Substance from Cheonggukjang. J. Agri. Life Sci., 44(2): 10-14
  38. Yang HR, Hong SR. 2014. Lentil beans diet Recipe. Chungrim Life, Seoul, Korea, pp 70-79
  39. Youn KC, Kim DH, Kim JO, Park BJ, Yook HS, Cho JM, Byun MW. 2002. Quality characteristics of the Chungkookjang fermented by the mixed culture of Bacillus natto and B. licheniformis. J. Korean Soc. Food Sci. Nutr., 31(2):204-210. https://doi.org/10.3746/jkfn.2002.31.2.204
  40. Codex. 2015. Ministry of Food and Drug Safety. Available from: http://www.fao.org/fao-who-codexalimentarius. Accessed November 23, 2016