The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Involvement of a Polyketide Synthetase ClPKS18 in the Regulation of Vegetative Growth, Melanin and Toxin Synthesis, and Virulence in Curvularia lunata

  • Gao, Jin-Xin (School of Agriculture and Biology, Shanghai Jiaotong University) ;
  • Chen, Jie (School of Agriculture and Biology, Shanghai Jiaotong University)
  • Received : 2017.04.11
  • Accepted : 2017.06.12
  • Published : 2017.12.01
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Abstract

The clpks18 gene was first cloned and identified in Curvularia lunata. It contains 6571 base pairs (bp) and an 6276 bp open reading frame encoding 2091 amino acids. The ClPKS18 deletion mutant displayed an albino phenotype, and almost lost the ability to product 5-(hydroxymethyl) furan-2-carboxylate (M5HF2C) toxin, implying that clpks18 gene in C. lunata is not only involved in 1,8-dihydroxynaphthalene melanin synthesis, but also relatively associated with M5HF2C toxin biosynthesis of the pathogen. The pathogenicity assays revealed that ${\Delta}ClPKS18$ was impaired in colonizing the maize leaves, which corresponds to the finding that ClPKS18 controls the production of melanin and M5HF2C in C. lunata. Results indicate that ClPKS18 plays a vital role in regulating pathogenicity of in C. lunata.

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References

  1. Eliahu, N., Igbaria, A., Rose, M. S., Horwitz, B. A. and Lev, S. 2007. Melanin biosynthesis in the maize pathogen Cochliobolus heterostrophus depends on two mitogen-activated protein kinases, Chk1 and Mps1, and the transcription factor Cmr1. Eukaryot. Cell 6:421-429. https://doi.org/10.1128/EC.00264-06
  2. Gao, J. X., Gao, S. G., Li, Y. Q. and Chen, J. 2015a. Genomewide prediction and analysis of the classical secreted proteins of Curvularia lunata. J. Plant. Prot. 42:869-876.
  3. Gao, J. X., Jing, J. and Chen, J. 2015b. Elementary coordinated expression research on genes related to the synthesis of pathogenesis-related melanin and toxin in Cochliobolus lunatus. J. SJTU Agr. Sci. 33:53-58.
  4. Gao, J. X., Jing, J., Liu, T. and Chen, J. 2015c. Identification of Clt-1-regulated proteins associated with the production of non-host-specific toxin and pathogenicity in Cochliobolus lunatus. J. Phytopathol. 163:33-41. https://doi.org/10.1111/jph.12278
  5. Gao, J. X., Jing, J., Liu, T., Yu, C. J., Li, Y. Q. and Chen, J. 2015d. Identification of proteins associated with the production of melanin and with pathogenicity in maize pathogen Curvularia lunata. Australas. Plant Pathol. 44:599-603. https://doi.org/10.1007/s13313-015-0382-3
  6. Gao, J. X., Jing, J., Yu, C. J. and Chen, J. 2015e. Construction of a high-quality yeast two-hybrid library and its application in identification of interacting proteins with Brn1 in Curvularia lunata. Plant Pathol. J. 31:108-114. https://doi.org/10.5423/PPJ.OA.01.2015.0001
  7. Gao, J. X., Liu, T. and Chen, J. 2014a. Insertional mutagenesis and cloning of the gene required for the biosynthesis of the non-host-specific toxin in Cochliobolus lunatus that causes maize leaf spot. Phytopathology 104:332-339. https://doi.org/10.1094/PHYTO-07-13-0190-R
  8. Gao, J. X., Yu, C. J., Wang, M., Sun, J. N., Li, Y. Q. and Chen, J. 2017. Involvement of a velvet protein ClVelB in the regulation of vegetative differentiation, oxidative stress response, secondary metabolism, and virulence in Curvularia lunata. Sci. Rep. 7:46054. https://doi.org/10.1038/srep46054
  9. Gao, S. G., Li, Y. Q., Gao, J. X., Suo, Y. J., Fu, K. H., Li, Y. Y. and Chen, J. 2014b. Genome sequence and virulence variation-related transcriptome profiles of Curvularia lunata, an important maize pathogenic fungus. BMC Genomics 15:627. https://doi.org/10.1186/1471-2164-15-627
  10. Gao, S. G., Liu, T., Li, Y. Y., Wu, Q., Fu, K. H. and Chen, J. 2012. Understanding resistant germplasm-induced virulence variation through analysis of proteomics and suppression subtractive hybridization in a maize pathogen Curvularia lunata. Proteomics 12: 3524-3535. https://doi.org/10.1002/pmic.201200105
  11. Liu, T., Liu, L. X., Jiang, X., Huang, X. L. and Chen, J. 2009. A new furanoid toxin produced by Curvularia lunata, the causal agent of maize Curvularia leaf spot. Can. J. Plant Pathol. 31:22-27. https://doi.org/10.1080/07060660909507568
  12. Liu, T., Wang, Y. Y., Ma, B. C., Hou, J. M., Jin, Y. Z., Zhang, Y. L., Ke, X. W., Tai, L. M., Zuo, Y. H. and Dey, K. 2016. Clg2p interacts with Clf and ClUrase to regulate appressorium formation, pathogenicity and conidial morphology in Curvularia lunata. Sci. Rep. 6:24047. https://doi.org/10.1038/srep24047
  13. Liu, T., Xu, S. F., Liu, L. X., Zhou, F. H., Hou, J. M. and Chen, J. 2011. Cloning and characteristics of Brn1 gene in Curvularia lunata causing leaf spot in maize. Eur. J. Plant Pathol. 131:211-219. https://doi.org/10.1007/s10658-011-9800-8
  14. Yan, H., Chen, J., Gao, Z., Xia, S. and Zhang, R. 2005. The heredity and variation of the interaction between Curvularia Lunata and Plant. J. Maize Sci. 13:119-120.