Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Enhancement of Ginsenoside Rg1 and Rg5 Contents in an Extract of Wood-cultivated Ginseng by Lactobacillus plantarum

Lactobacillus plantarum을 이용한 산양삼 추출물의 진세노사이드 Rg1 및 Rg5의 함량 증대

  • Kwon, Hun-Joo (Department of Food Science and Biotechnology, Kangwon National University) ;
  • Cho, Yun-Ji (Department of Food Science and Biotechnology, Kangwon National University) ;
  • Kim, Myoung-Dong (Department of Food Science and Biotechnology, Kangwon National University)
  • 권훈주 (강원대학교식품생명공학과) ;
  • 조윤지 (강원대학교식품생명공학과) ;
  • 김명동 (강원대학교식품생명공학과)
  • Received : 2017.11.07
  • Accepted : 2017.11.30
  • Published : 2017.12.28
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Abstract

Twelve lactic acid bacteria harboring ${\alpha}$-rhamnosidase (EC 3.2.1.40) activity were isolated from traditional Korean foods. The 6 strains (Weissella confuse [n = 1], Lactobacillus pentosus [n = 1], and Lactobacillus plantarum [n = 4]) with the highest rhamnosidase activity were selected for bioconversion of an extract of wood-cultivated ginseng. The L. plantarum MBE/L2990 strain increased ginsenoside content (0.58 mg for Rg1 and 0.24 mg for Rg5) and showed higher bioconversion activity than the control strain L. plantarum KCTC21004 (56% and 42% increase for Rg1 and Rg5, respectively). L. plantarum MBE/L2990 was deposited at the Korean Collection for Type Cultures as Lactobacillus plantarum KCTC18529P.

발효식품으로부터 분리한 젖산균 중 ${\alpha}$-rhamnosidase 효소활성을 보유한 젖산균 12점을 선별하였다. 효소활성이 우수한 Weissella confuse 1점, L. pentosus 1점과 효소활성이 우수하였던 4점의 L. plantarum 균주를 사용하여 진세노사이드 Rb1과 Re를 Rg1, Rg5로 각각 생물전환하였다. L. plantarum MBE/L2990 균주를 사용한 생물전환반응에서 Rg1과 Rg5의 함량이 각각 0.58 mg, 0.24 mg 가량 증가하였으며, 대조구로 사용한 L. plantarum KCTC21004 균주에 비해 약 56% 및 42% 우수한 생물전환 효율이었다. L. plantarum MBE/L2990 균주는 한국미생물자원센터에 KCTC18529P로 기탁하였다.

Keywords

References

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