Korean Journal of Poultry Science (한국가금학회지)

The Korean Society of Poultry Science (한국가금학회)
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Biochemical and Histopathological Study of Aflatoxicosis on Ross 308 Broiler Chicks

  • Received : 2017.11.03
  • Accepted : 2017.12.12
  • Published : 2017.12.31
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Abstract

Totally, one hundred and sixty 1-day-old Ross 308 broiler chicks were fed with a diet containing 0, 0.5, 1.0, and 2.0 mg of aflatoxin $B_1(AFB_1)/kg$ of feed for 21 days. Body weight was lower for the $AFB_1$-treated broilers than for the control group. At 14 and 21 DPF, the broilers fed with 2.0 mg of $AFB_1/kg$ of feed weighed significantly lower than those of the other groups (p<0.05). Relative liver weights increased significantly in a dose-dependent manner, and relative spleen weights were significantly high in the chicks fed with 2.0 mg of $AFB_1/kg$ of feed at 21 DPF (p<0.001). Biochemical analyses showed that total protein and albumin levels decreased significantly at 7 and 14 DPF for the chicks of the group fed with 2.0 of mg $AFB_1/kg$ of feed, compared with those fed with 0.5 and 1.0 mg of $AFB_1/kg$ of feed (p<0.05). AST and ALT levels increased significantly at 14 and 21 DPF (p<0.05), and the AST levels, particularly, increased dose-dependently (p<0.05). Histopathological analyses showed that the liver tissues of the $AFB_1$-treated chicks showed significant lesions, including hemorrhage, hepatocyte necrosis, inflammatory cell infiltration, and fatty degeneration. The severity of both hepatocyte necrosis and inflammatory cell infiltration appeared to increase dose- and time-dependently. Similarly, hepatic fibrosis increased dose-dependently (p<0.05). The results of this study could improve our understanding of parameters used for evaluating aflatoxicosis in poultry.

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References

  1. Chen X, Horn N, Applegate TJ 2014 Efficiency of hydrated sodium calcium aluminosilicate to ameliorate the adverse effects of graded levels of aflatoxin $B_1$ in broiler chicks. Poult Sci 93:2037-2047. https://doi.org/10.3382/ps.2014-03984
  2. Diaz GJ, Calabrese E, Blain R 2008 Aflatoxicosis in chickens (Gallus gallus): An example of hormesis? Poult Sci 87:727-732. https://doi.org/10.3382/ps.2007-00403
  3. Fowler J, Li W, Bailey C 2015 Effects of a calcium bentonite clay in diets containing aflatoxin when measuring liver residues of aflatoxin $B_1$ in starter broiler chicks. Toxins (Basel) 7:3455-3464. https://doi.org/10.3390/toxins7093455
  4. Huff WE, Kubena LF, Harvey RB, Corrier DE, Mollenhauer HH 1986 Progression of aflatoxicosis in broiler chickens. Poult Sci 65:1891-1899. https://doi.org/10.3382/ps.0651891
  5. Hussain Z, Rehman HU, Manzoor S, Tahir S, Mukhtar M 2016 Determination of liver and muscle aflatoxin $B_1$ residues and select serum chemistry variables during chronic aflatoxicosis in broiler chickens. Vet Clin Pathol 45:330-334. https://doi.org/10.1111/vcp.12336
  6. IARC 2012 Aflatoxins. In A review of human carcinogen. Part. F: Chemical Agents and Related Occupations; International Agency for Research on Cancer Working Group on the Evaluation of Carcinogenic Risks to Human. Lyon, France.
  7. Kaoud HA 2013 Innovative methods for the amelioration of aflatoxin (afb1) effect in broiler chicks. Sci J Appl Res 1:15-19.
  8. Kececi T, Oguz H, Kurtoglu V, Demet O 1998 Effects of polyvinylpolyprolidone, synthetic zeolite and bentonite on serum biochemical and haematological characters of broiler chickens during aflatoxicosis. Br Poult Sci. 39:452-458. https://doi.org/10.1080/00071669889051
  9. Kensler TW, Roebuck BD, Wogan GN, Groopman JD 2011 Aflatoxin: A 50-year odyssey of mechanistic and translational toxicology. Toxicol Sci 120:S28-S48. https://doi.org/10.1093/toxsci/kfq283
  10. Magnoli AP, Monge MP, Miazzo RD, Cavaglieri LR, Magnoli CE, Merkis CI, Cristofolini AL, Dalcero AM, Chiacchiera SM 2011 Effect of low levels of aflatoxin $B_1$ on performance, biochemical parameters, and aflatoxin $B_1$ in broiler liver tissues in the presence of monensin and sodium bentonite. Poult Sci 90:48-58. https://doi.org/10.3382/ps.2010-00971
  11. Monson MS, Coulombe RA, Reed KM 2015 Aflatoxicosis: Lessons from toxicity and responses to aflatoxin $B_1$ in poultry. Agriculture 5:742-777. https://doi.org/10.3390/agriculture5030742
  12. Oguz H, Kececi T, Birdane YO, Onder F, Kurtoglu V 2000 Effect of clinoptilolite on serum biochemical and haematological characters of broiler chickens during aflatoxicosis. Res Vet Sci 69:89-93. https://doi.org/10.1053/rvsc.2000.0395
  13. Pandey I, Chauhan SS 2007 Studies on production performance and toxin residues in tissues and eggs of layer chickens fed on diets with various concentrations of aflatoxin $AFB_1$. Br Sci 48:713-723.
  14. Peng X, Zhang K, Bai S, Ding X, Zeng Q, Yang J, Fang J, Chen K 2014a Histological lesions, cell cyclear rest, apoptosis and T cell subsets changes of spleen in chicken fed aflatoxin-contaminated corn. Int J Environ Res Public Health 11:8567-8580. https://doi.org/10.3390/ijerph110808567
  15. Peng X, Zhang S, Fang J, Cui H, Zuo Z, Deng J 2014b Protective roles of sodium selenite against aflatoxin $B_1$-induced apoptosis of jejunumin broilers. Int J Environ Res Public Health 11:13130-13143. https://doi.org/10.3390/ijerph111213130
  16. Raju MV, Devegowda G 2000 Influence of esterified-glucomannan on performance and organ morphology, serum biochemistry and haematology in broilers exposed to individual and combined mycotoxicosis (aflatoxin, ochratoxin and T-2 toxin). Br Poult Sci 41:640-650. https://doi.org/10.1080/713654986
  17. Rawal S, Kim JE, Coulombe R 2010 Aflatoxin B1 in poultry: Toxicology, metabolism and prevention. Res Vet Sci 89:325-331. https://doi.org/10.1016/j.rvsc.2010.04.011
  18. Tedesco D, Steidler S, Galletti S, Tameni M, Sonzogni O, Ravarotto L 2004 Efficacy of silymarin-phospholipid complex in reducing the toxicity of aflatoxin $B_1$ in broiler chicks. Poult Sci 83:1839-1843. https://doi.org/10.1093/ps/83.11.1839
  19. Tessari EN, Kobashigawa E, Cardoso AL, Ledoux DR, Rottinghaus GE, Oliveira CA 2010 Effects of aflatoxin $B_1$ and fumonis in $B_1$ on blood biochemical parameters in broilers. Toxins (Basel) 2:453-460. https://doi.org/10.3390/toxins2040453
  20. Valdivia A, Martinez A, Damian F, Quezada T, Ortiz R, Martinez C, Llamas J, Rodriguez ML, Yamamoto L, Jaramillo F, Loarca-Pina MG, Reyes JL 2001 Efficacy of N-acetylcysteine to reduce the effects of aflatoxin $B_1$ in toxic ationin broiler chickens. Poult Sci 80:727-734. https://doi.org/10.1093/ps/80.6.727
  21. Wogan GN 1992 Aflatoxins as risk factors for hepatocellular carcinoma in humans. Cancer Res 52:S2114-S2118.
  22. Yunus AW, Razzazi-Fazeli E, Bohm J 2011 Aflatoxin $B_1$ in affecting broiler's performance, immunity, and gastrointestinal tract: A review of history and contemporary issues. Toxins (Basel) 3:566-590. https://doi.org/10.3390/toxins3060566
  23. Zhao J, Shirley RB, Dibner JD, Uraizee F, Officer M, Kitchell M, Vazquez-Anon M, Knight CD 2010 Comparison of hydrated sodium calcium aluminosilicate and yeast cell wall on counteracting aflatoxicosis in broiler chicks. Poult Sci 89:2147-2156. https://doi.org/10.3382/ps.2009-00608