한국약용작물학회지 (Korean Journal of Medicinal Crop Science)

  • 제25권6호
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  • Pages.389-396
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  • 2017
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  • 1225-9306(pISSN)
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  • 2288-0186(eISSN)
한국약용작물학회 (The Korean Society of Medicinal Crop Science)
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SSR 마커를 이용한 산양삼의 유전적 다양성 분석

Genetic Diversity Analysis of Wood-cultivated Ginseng using Simple Sequence Repeat Markers

  • 길진수 (충북대학교 특용식물학과) ;
  • 엄유리 (국립산림과학원 산림약용자원연구소) ;
  • 변재경 (한국임업진흥원) ;
  • 정종욱 (충북대학교 특용식물학과) ;
  • 이이 (충북대학교 특용식물학과) ;
  • 정찬문 (충북대학교 특용식물학과)
  • Gil, Jinsu (Department of Industrial Plant Science and Technology, Chungbuk National University) ;
  • Um, Yurry (Forest Medicinal Research Center, NIFS) ;
  • Byun, Jae Kyung (Korea Forest Promotion Institute) ;
  • Chung, Jong Wook (Department of Industrial Plant Science and Technology, Chungbuk National University) ;
  • Lee, Yi (Department of Industrial Plant Science and Technology, Chungbuk National University) ;
  • Chung, Chan Moon (Department of Industrial Plant Science and Technology, Chungbuk National University)
  • 투고 : 2017.07.14
  • 심사 : 2017.10.30
  • 발행 : 2017.12.30
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초록

Background: Panax ginseng C. A. Meyer is wood-cultivated ginseng (WCG) in Korea which depends on an artificial forest growth method. To produce this type of ginseng, various P. ginseng cultivars can be used. To obtain a WCG similar to wild ginseng (WG), this method is usually performed in a mountain using seeds or seedlings of cultivated ginseng (CG) and WG. Recently, the WCG industry is suffering a problem in that Panax notoginseng (Burk.) F. H. Chen or Panax quinquefolium L. are being sold as WCG Korean market; These morphological similarities have created confusion among customers. Methods and Results: WCG samples were collected from five areas in Korea. After polymerase chain reaction (PCR) amplification using the primer pair labeled with fluorescence dye (FAM, NED, PET, or VIC), fragment analysis were performed. PCR products were separated by capillary electrophoresis with an ABI 3730 DNA analyzer. From the results, WCG cultivated in Korea showed very diverse genetic background. Conclusions: In this study, we tried to develop a method to discriminate between WCG, P. notoginseng or P. quinquefolium using simple sequence repeat (SSR) markers. Furthermore, we analyzed the genetic diversity of WCG collected from five cultivation areas in Korea.

키워드

참고문헌

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