한국초지조사료학회지 (Journal of The Korean Society of Grassland and Forage Science)

  • 제37권4호
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  • Pages.322-331
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  • 2017
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  • 2287-5824(pISSN)
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  • 2287-5832(eISSN)
한국초지조사료학회 (The Korean Society of Grassland and Forage Science)
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말 분변을 이용한 주스박의 in vitro 발효 특성

In vitro Fermentation Characteristics of Juice Pomaces Using Equine Fecal Inoculum

  • 황원욱 (건국대학교 동물자원과학과) ;
  • 김겸헌 (건국대학교 동물자원과학과) ;
  • 임정호 (건국대학교 동물자원과학과) ;
  • 우제훈 (농촌진흥청 국립축산과학원 난지축산시험장) ;
  • 박남건 (농촌진흥청 국립축산과학원 난지축산시험장) ;
  • 김수기 (건국대학교 동물자원과학과)
  • Hwang, Won-Uk (Department of Animal Science and Technology, Konkuk University) ;
  • Kim, Gyeom-Heon (Department of Animal Science and Technology, Konkuk University) ;
  • Lim, Joung-Ho (Department of Animal Science and Technology, Konkuk University) ;
  • Woo, Jae-Hoon (National Institute of Animal Science, RDA) ;
  • Park, Nam-Geon (National Institute of Animal Science, RDA) ;
  • Kim, Soo-Ki (Department of Animal Science and Technology, Konkuk University)
  • 투고 : 2017.08.26
  • 심사 : 2017.11.13
  • 발행 : 2017.12.31
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초록

본 연구는 바실러스, 효모 그리고 유산균으로 발효한 다양한 주스박들과 말 분변을 첨가한 in vitro 발효 시험을 통해 pH, 건물 분해율, 암모니아성 질소, 가스 발생량, 휘발성 지방산의 변화를 조사하였다. 주스박 첨가에 따른 발효액의 pH의 범위는 6.4~7.1로 미생물 발효에 의한 소화가 정상적으로 나타났다. 48시간 후 발효액에서 $10^9CFU/m{\ell}$ 정도 미생물이 증식함에 따라 사용한 주스박들이 장내 미생물에 의한 소화작용에 유용하게 이용될 것으로 판단되었다. 건물 분해율은 배양 12시간 이후부터 증가하여 48시간째에 사과박 39.19%, 포도박 38.22%, 당근박 37.02%, 감귤박 36.2% 그리고 혼합박 34.35%로 나타났다. 암모니아성 질소의 농도는 배양 12시간까지는 전 처리구에서 $1.5mg/100m{\ell}$의 수준이었으며 배양 24시간 이후부터 급격히 증가하였다. 가스 발생량은 혼합박이 가장 낮았으며, 배양 12시간 이후부터 급격히 증가하였다. 총 VFA는 배양 24시간부터 증가하여 48시간에 가장 높았다. 본 연구결과로부터 발효 주스박은 말 in vitro 소화과정에서 적정 pH를 유지하면서 건물 분해가 진행되었고 말의 맹장과 대장에서 섬유소 분해 미생물의 작용이 활발히 이루어질 수 있음을 제시하였다.

This study was conducted to evaluate the changes of pH, dry matter digestibility (DMD), $NH_3-N$ concentrate, gas production and volatile fatty acid (VFA) through in vitro fermentation by adding horse feces to various juice pomaces fermented with Bacillus, yeast and lactic acid bacteria. The pH range of fermented fluid with juice pomaces was 6.4-7.1, indicating that the digestion by microbial fermentation was normal. Juice pomaces adopted will be helpfully used to assist with digestion by microbes in intestines because approximately $10^9CFU/m{\ell}$ microbes were grown after 48 hours in fermented fluid. DMD rate gradually increased from 12 hours. It was 39.19% in pomaces of apple, 38.22% in grape, 37.02% in carrot, 36.2% in citrus and 34.35% in mixture respectively after 48 hours. $NH_3-N$ concentrate was not changed significantly as it was maintained at $1.5mg/100m{\ell}$ level in the entire treatment group from beginning of fermentation until 12 hours, but increased rapidly from 24 hours. Amount of gas produced was lowest in the mixture and increased rapidly after 12 hours. Total VFA increased from 24 hours and was highest at 48 hours. It was suggested that dry matter digestion was processed while fermented juice pomaces kept proper pH during in vitro digestion, and cellulose degrading microorganisms could act actively in the caecum and colon of horses.

키워드

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