Korean Journal of Clinical Laboratory Science (대한임상검사과학회지)

Korean Society for Clinical Laboratory Science (대한임상검사과학회)
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Comparison of the miR-23b and miR-203 Expressions in Endometrial Cancer

자궁내막암종에서 miR-23b와 miR-203 발현 비교

  • Lee, Kyung Eun (Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan)
  • 이경은 (부산가톨릭대학교 보건과학대학 임상병리학과)
  • Received : 2017.10.19
  • Accepted : 2017.10.30
  • Published : 2017.12.31
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Abstract

MicroRNAs (miRNAs/miRs) are a group of small noncoding RNAs that modulate gene expression. Many studies, demonstrating altered expressions of specific miRNAs in diverse types of human neoplasia, suggested that they may play a key role in tumorigenesis. Recently, miRNA genes were found to be abnormally expressed in several types of cancer, including endometrial cancer. However, miR-23b and miR-203 expression in endometrial cancer has yet to be studied in Korea. As such, the purpose of this study was to analyze miR-23b and miR-203 expressions in endometrial cancer and to evaluate the relationship between miR-23b and miR-203 expressions. A retrospective study was carried out on the formalin-fixed, paraffin-embedded tissues of 42 endometrial cancer tissues using quantitative real-time PCR. In endometrial cancer tissues, miR-23b expression levels ($2.70{\pm}4.45$) were higher than miR-203 expression levels ($-2.34{\pm}4.08$). Endometrial cancer tissues showed an overexpression of miR-23b in 30 (71.4%) of the 42 endometrial cancer cases, whereas miR-203 was underexpressed in 29 (69.0%) of the 42 cases. There was a significant association between miR-23b and miR-203 expressions in endometrial cancer tissues (p=0.0005). These findings suggest that miR-23b and miR-203 expressions may be involved in endometrial carcinogenesis. More studies are needed to further define the relationship between miR-23b and miR-203 expressions and tissue-specific protein expression.

MicroRNA는 작은 비암호화 RNA로서 유전자 발현을 조절한다. 다양한 인체 종양에서 특이 miRNA 발현의 변화가 보고되면서 종양 발생에 중요한 역할을 수행하는 것으로 알려졌다. 최근에는 자궁내막암종을 포함한 다양한 암종에서 여러 miRNA의 비정상적인 발현이 보고되었으나, miR-23b와 miR-203 발현에 대한 연구 결과는 아직 국내에 보고되지 않았다. 따라서 본 연구에서는 자궁내막암종에서 miR-23b와 miR-203의 발현을 비교하고 상호 연관성을 분석하고자 하였다. 인체 자궁내막암종으로 진단된 파라핀 블록 42건을 대상으로 quantitative real-time PCR을 이용하여 miRNA 발현 수치를 분석하였다. miR-23b 발현 수치는 $2.70{\pm}4.45$로 miR-203의 발현 수치 $-2.34{\pm}4.08$ 보다 높게 나타났다. 또한 miR-23b는 총 42건 중 30건(71.4%)에서 양의 발현이 나타났고, miR-203은 총 42건 중 29건(69.0%)에서 음의 발현이 나타났으며, 이는 통계학적으로 유의한 차이를 나타냈다(p=0.0005). 따라서 본 연구에서는 miR-23b와 miR-203 발현은 자궁내막암종 발생에 연관이 있을 것으로 추정되며, 향후 miR-23b 와 miR-203 발현과 조직특이 단백 발현과의 상호 연관성에 대한 연구가 더 필요할 것으로 사료된다.

Keywords

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