Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Anti-oxidative Effect of Epimedii Herba in Caenorhabditis elegans

음양곽의 예쁜꼬마선충 내의 항산화 효과

  • Received : 2017.11.29
  • Accepted : 2017.12.12
  • Published : 2017.12.29
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Abstract

To know the anti-oxidative effect of Epimedii Herba (Berberidaceae), the methanol extract of this plant was investigated by using a Caenorhabditis elegans model system. The methanol extract of this plant showed relatively significant DPPH radical scavenging and superoxide quenching activities. The ethyl acetate soluble fraction of Epimedii Herba (EHE), which showed the most potent DPPH radical scavenging and superoxide quenching activities, was tested on its effects on superoxide dismutase (SOD), catalase, intracellular ROS, and oxidative stress tolerance in Caenorhabditis elegans. Furthermore, in order to verify that regulation of stress-response genes is responsible for the increased stress tolerance of the EHE treated C. elegans, we checked SOD-3 expression using a transgenic strain. As a result, the EHE increased SOD and catalase activities of C. elegans, and reduced intracellular ROS accumulation in a dose-dependent manner. Besides, EHE-treated CF1553 worms showed higher SOD-3::GFP intensity than that of non-treated controls.

Keywords

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