Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Effects of antioxidants on viability, plasma membrane integrity and apoptosis in porcine ovarian granulosa cells damaged by bromopropane

항산화제가 Bromopropane에 의해 손상된 돼지 과립막세포의 생존율, 원형질막 온전성 및 apoptosis에 미치는 영향

  • Lee, Seunghyung (College of Animal Life Sciences, Kangwon National University) ;
  • Park, Hee-Woo (College of Animal Life Sciences, Kangwon National University) ;
  • Lee, Sang-Hee (College of Animal Life Sciences, Kangwon National University) ;
  • Cheong, Hee-Tae (School of Veterinary Medicine, Kangwon National University) ;
  • Park, Choon-Keun (College of Animal Life Sciences, Kangwon National University) ;
  • Yang, Boo-Keun (College of Animal Life Sciences, Kangwon National University)
  • 이승형 (강원대학교 동물생명과학대학) ;
  • 박희우 (강원대학교 동물생명과학대학) ;
  • 이상희 (강원대학교 동물생명과학대학) ;
  • 정희태 (강원대학교 수의과대학) ;
  • 박춘근 (강원대학교 동물생명과학대학) ;
  • 양부근 (강원대학교 동물생명과학대학)
  • Received : 2016.03.21
  • Accepted : 2016.06.22
  • Published : 2016.09.30
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Abstract

The purpose of this study was to examine the effects of taurine and vitamin E on ovarian granulosa cells damaged by bromopropane (BP) in pigs. We evaluated cell viability, plasma membrane integrity (PMI) and apoptotic morphological change in porcine ovarian granulosa cells. The cells were treated with 1-BP (0, 5.0, 10, and $50{\mu}M$), 2-BP (0, 5.0, 10, and 50 mM), taurine (0, 5.0, 10, and 25 mM), and vitamin E (0, 100, 200, and $400{\mu}M$) for 24 h. $10{\mu}M$ 1-BP and $50{\mu}M$ 2-BP inhibited viability and PMI, and induced apoptosis in porcine ovarian granulosa cells (p < 0.05). Cell viability and PMI were increased by taurine (10 and 25 mM) and vitamin E (100 and $200{\mu}M$), and apoptosis decreased (p < 0.05). Finally, the porcine ovarian granulosa cells were co-treated with BPs ($10{\mu}M$), taurine (10 mM) and/or vitamin E ($200{\mu}M$). Cell viability and PMI in the co-treated cells were increased, and apoptosis was decreased. In conclusion, taurine and vitamin E can improve cell viability and inhibition of apoptosis in porcine ovarian granulosa cells damaged by bromopropane.

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References

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