Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Chemotactic Response Study of Cylindrocarpon destructans towards Ginseng Root Exudates

인삼 추출물에 의한 Cylindrocarpon destructans의 주화성 반응 연구

  • Xu, Yonghua (College of Chinese Medicinal Materials, Jilin Agricultural University) ;
  • Chi, Kun (College of Chinese Medicinal Materials, Jilin Agricultural University) ;
  • Zhang, Aihua (College of Chinese Medicinal Materials, Jilin Agricultural University) ;
  • Lei, Fengjie (College of Chinese Medicinal Materials, Jilin Agricultural University) ;
  • Yang, He (College of Chinese Medicinal Materials, Jilin Agricultural University) ;
  • Zhao, Yan (College of Chinese Medicinal Materials, Jilin Agricultural University) ;
  • Li, Kuo (College of Chinese Medicinal Materials, Jilin Agricultural University) ;
  • Wang, Erhuan (College of Chinese Medicinal Materials, Jilin Agricultural University) ;
  • Li, Qiong (College of Chinese Medicinal Materials, Jilin Agricultural University) ;
  • Kim, Jong Seog (Department of Rehabilitation, Woosuk University) ;
  • Lee, Seung Ho (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Kim, Young Chang (Department of Herbal Crop Research, NIHHS, RDA)
  • 허영화 (중국 길림농업대학교 중약재학원) ;
  • 시곤 (중국 길림농업대학교 중약재학원) ;
  • 장애화 (중국 길림농업대학교 중약재학원) ;
  • 뢰봉걸 (중국 길림농업대학교 중약재학원) ;
  • 양학 (중국 길림농업대학교 중약재학원) ;
  • 조암 (중국 길림농업대학교 중약재학원) ;
  • 이활 (중국 길림농업대학교 중약재학원) ;
  • 왕이환 (중국 길림농업대학교 중약재학원) ;
  • 이충 (중국 길림농업대학교 중약재학원) ;
  • 김종석 (우석대학교 재활학과) ;
  • 이승호 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김영창 (농촌진흥청 국립원예특작과학원 인삼특작부)
  • Received : 2016.06.06
  • Accepted : 2016.09.28
  • Published : 2016.10.30
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Abstract

Background: Cylindrocarpon destructans (Zins) Scholten is an important pathogenic fungus that causes ginseng root rot in many ginseng growing areas in China. Although C. destructans have been studied worldwide, research on its chemotaxis towards ginseng (Panax ginseng C. A. Meyer) root exudates in the rhizosphere remains limited. Methods and Results: In this study, we collected ginseng root exudates with three different polarities from three-year-old ginseng roots, and performed chemotaxis and spore germination assays to investigate the ability of these exudates to induce the response in C. destructans. The results showed that, compared with other conditions, when C. destructans cultivated at $20^{\circ}C$ and a pH of 6 exhibited a strong positive chemotactic response toward $2mg/{\ell}$ aqueous phase, $20mg/{\ell}$ butanol phase, and $0.2mg/{\ell}$ petroleum ether from ginseng root exudates, the chemotactic moving indexes were 0.1581, 0.1638 and 0.1441, respectively. In addition, the spore germination rate with optimal chemotactic parameters were 48%, 53%, and 41% in the aqueous phase, butanol phase and petroleum ether groups, respectiviely, which were significantly higher than that in the control group (23%) (p < 0.05). The mycelial growth rate with optimal chemotactic parameters increased with culture time, and the maximum growth rates in the aqueous phase, butanol phase and petroleum ether groups were 0.425, 0.406 and 0.364 respectively, on the 4th day. The optimal chemotactic parameters were $39.73mg/50mg/{\ell}$, $48.93mg/50mg/{\ell}$, and $31.43mg/50mg/{\ell}$, in aqueous phase, butanol phase and petroleum ether respectively, from ginseng root exudates, compared with $5.5mg/50mg/{\ell}$, in the control group. Conclusions: The present study revealed that certain ginseng root exudates containing chemical attractants act as nutritional sources or signals for C. destructans and support its colonization of ginseng roots.

Keywords

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