DOI QR코드

DOI QR Code

Effects of Roasting and Peeling Process and Extraction Temperature on the Antioxidant Activity of Burdock Tea

우엉차의 껍질 제거와 로스팅에 따른 추출온도별 항산화 효과

  • Park, Mi Young (Department of Food and Nutrition Education, Graduate School of Education, Soonchunhyang University) ;
  • Yu, Chengguang (Department of Food Science and Nutrition, Soonchunhyang University) ;
  • Park, Young Hyun (Department of Food Science and Nutrition, Soonchunhyang University)
  • 박미영 (순천향대학교 교육대학원 영양교육전공) ;
  • 우등광 (순천향대학교 자연과학대학 식품영양학과) ;
  • 박영현 (순천향대학교 자연과학대학 식품영양학과)
  • Received : 2016.04.12
  • Accepted : 2016.08.02
  • Published : 2016.10.30

Abstract

Background: We investigated the optimal aqueous extraction conditions for recovery of high yields of total phenolic compounds from roots of Arctium lappa L. (burdock, Asteraceae), and we compared their antioxidant capacity. Methods and Results: The antioxidant activity of the extracts was tested using 2,2-diphenyl-1-picrylhydrazyl, 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)-diammonium salt, and oxygen radical absorbance capacity assays. In addition, the major phenolic compounds present in the extracts were determined by high performance liquid chromatography analysis. Our results suggest that the roasted burdock $100^{\circ}C$, 15 min extract exhibited the strongest radical scavenging activity and possessed the highest concentration of phenolic compounds. The polyphenol content of both dried burdock and roasted burdock significantly increased with increase in the extraction temperature and time. Conclusions: These results indicated a relationship between phenolic compound levels in burdock and their free radical scavenging activities. This suggests that phenolic compounds significantly increase the antioxidant potential of burdock extracts.

Keywords

References

  1. Ahmed D, Fatima K and Saeed R. (2014). Analysis of phenolic and flavonoid contents, and the anti-oxidative potential and lipid peroxidation inhibitory activity of methanolic extract of Carissa opaca roots and its fractions in different solvents. Antioxidants. 3:671-683. https://doi.org/10.3390/antiox3040671
  2. Ainsworth EA and Gillespie KM. (2007). Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin-Ciocalteu reagent. Nature Protocols. 2:875-877. https://doi.org/10.1038/nprot.2007.102
  3. Asikin Y, Taira I, Inafuku S, Sumi H, Sawamura M, Takara K and Wada K. (2012). Volatile aroma components and antioxidant activities of the flavedo peel extract of unripe Shiikuwasha(Citrus depressa Hayata). Journal of Food Science. 77:C469-C475. https://doi.org/10.1111/j.1750-3841.2011.02604.x
  4. Braca A, de Tommasi N, di Bari L, Pizza C, Politi M and Morelli I. (2001). Antioxidant principles from Bauhinia tarapotensis. Journal of Natural Products. 64:892-895. https://doi.org/10.1021/np0100845
  5. Chukwumah Y, Walker LT and Verghese M. (2009). Peanut skin color: A biomarker for total polyphenolic content and antioxidative capacities of peanut cultivars. International Journal of Molecular Sciences. 10:4941-4952. https://doi.org/10.3390/ijms10114941
  6. da Silva LM, Allemand A, Mendes DAGB, dos Santos AC, Andre E, de Souza LM, Cipriani TR, Dartora N, Marques MCA, Baggio CH and Werner MF. (2013). Ethanolic extract of roots from Arctium lappa L. accelerates the healing of acetic acid-induced gastric ulcer in rats: Involvement of the antioxidant system. Food and Chemical Toxicology. 51:179-187. https://doi.org/10.1016/j.fct.2012.09.026
  7. dos Santos AC, Baggio CH, Freitas CS, Lepieszynski J, Mayer B, Twardowschy A, Missau FC, dos Santos EP, Pizzolatti MG and Marques MC. (2008). Gastroprotective activity of the chloroform extract of the roots from Arctium lappa L. Journal of Pharmacy and Pharmacology. 60:795-801. https://doi.org/10.1211/jpp.60.6.0016
  8. Gate L, Paul J, Ba GN, Tew KD and Tapiero H. (1999). Oxidative stress induced in pathologies: The role of antioxidants. Biomedicine and Pharmacotherapy. 53:169-180. https://doi.org/10.1016/S0753-3322(99)80086-9
  9. Gorinstein S, Zachwieja Z, Folta M, Barton H, Piotrowicz J, Zemser M, Weisz M, Trakhtenberg S and Martin-Belloso O. (2001). Comparative contents of dietary fiber, total phenolics, and minerals in persimmons and apples. Journal of Agricultural and Food Chemistry. 49:952-957. https://doi.org/10.1021/jf000947k
  10. Gursoy N, Sihoglu-Tepe A and Tepe B. (2009). Determination of in vitro antioxidative and antimicrobial properties and total phenolic contents of Ziziphora clinopodioides, Cyclotrichium niveum, and Mentha longifolia ssp. typhoides var. typhoides. Journal of Medicinal Food. 12:684-689. https://doi.org/10.1089/jmf.2008.0102
  11. Halliwell B. (1996). Antioxidants in human health and disease. Annual Review of Nutrition. 16:33-50. https://doi.org/10.1146/annurev.nu.16.070196.000341
  12. Hotta H, Nagano S, Ueda M, Tsujino Y, Koyama J and Osakai T. (2002). Higher radical scavenging activities of polyphenolic antioxidants can be ascribed to chemical reactions following their oxidation. Biochimica et Biophysica Acta. 1572:123-132. https://doi.org/10.1016/S0304-4165(02)00285-4
  13. Huang TC, Tsai SS, Liu LF, Liu YL, Liu HJ and Chuang KP. (2010). Effect of Arctium lappa L. in the dextran sulfate sodium colitis mouse model. World Journal of Gastroenterology. 16:4193-4199. https://doi.org/10.3748/wjg.v16.i33.4193
  14. Jaiswal R and Kuhnert N. (2011). Identification and characterization of five new classes of chlorogenic acids in burdock(Arctium lappa L.) roots by liquid chromatography/tandem mass spectrometry. Food and Function. 2:63-71. https://doi.org/10.1039/C0FO00125B
  15. JianFeng C, PengYing Z, ChengWei X, TaoTao H, YunGui B and KaoShan C. (2012). Effect of aqueous extract of Arctium lappa L.(burdock) roots on the sexual behavior of male rats. BMC Complementary and Alternative Medicine. 12:8-8.
  16. Lee JH, Park AR, Choi DW, Kim JD, Kim JC, Ahn JH, Lee HY, Choe M, Choi KP, Shin IC and Park HJ. (2011). Analysis of chemical compositions and electron-donating ability of 4 Korean wild Sannamuls. Korean Journal of Medicinal Crop Science. 19:111-116. https://doi.org/10.7783/KJMCS.2011.19.2.111
  17. Li D, Kim JM, Jin Z and Zhou J. (2008). Prebiotic effectiveness of inulin extracted from edible burdock. Anaerobe. 14:29-34. https://doi.org/10.1016/j.anaerobe.2007.10.002
  18. Lin LZ and Harnly JM. (2008). Identification of hydroxycinnam oylquinic acids of arnica flowers and burdock roots using a standardized LC-DAD-ESI/MS profiling method. Journal of Agricultural and Food Chemistry. 56:10105-10114. https://doi.org/10.1021/jf802412m
  19. Liu J, Cai YZ, Wong RNS, Lee CKF, Tang SCW, Sze SCW, Tong Y and Zhang Y. (2012). Comparative analysis of caffeoylquinic acids and lignans in roots and seeds among various burdock(Arctium lappa) genotypes with high antioxidant activity. Journal of Agricultural and Food Chemistry. 60:4067-4075. https://doi.org/10.1021/jf2050697
  20. Liu W, Wang J, Zhang Z, Xu J, Xie Z, Slavin M and Gao X. (2014). In vitro and in vivo antioxidant activity of a fructan from the roots of Arctium lappa L. International Journal of Biological Macromolecules. 65:446-453. https://doi.org/10.1016/j.ijbiomac.2014.01.062
  21. Mustafa RA, Abdul Hamid A, Mohamed S and Bakar FA. (2010). Total phenolic compounds, flavonoids, and radical scavenging activity of 21 selected tropical plants. Journal of Food Science. 75:C28-C35. https://doi.org/10.1111/j.1750-3841.2009.01401.x
  22. Nordberg J and Arnr ES. (2001). Reactive oxygen species, antioxidants, and the mammalian thioredoxin system. Free Radical Biology and Medicine. 31:1287-1312. https://doi.org/10.1016/S0891-5849(01)00724-9
  23. Ou B, Hampsch-Woodill M and Prior RL. (2001). Development and validation of an improved oxygen radical absorbance capacity assay using fluorescein as the fluorescent probe. Journal of Agricultural and Food Chemistry. 49:4619-4626. https://doi.org/10.1021/jf010586o
  24. Pereira JV, Bergamo DCB, Pereira JO, Frana SDC, Pietro RCLR and Silva-Sousa YTC. (2005). Antimicrobial activity of Arctium lappa constituents against microorganisms commonly found in endodontic infections. Brazilian Dental Journal. 16:192-196. https://doi.org/10.1590/S0103-64402005000300004
  25. Prior RL, Wu X and Schaich K. (2005). Standardized methods for the determination of antioxidant capacity and phenolic in foods and dietary supplements. Journal of Agricultural and Food Chemistry. 53:4290-4302. https://doi.org/10.1021/jf0502698
  26. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M and Rice-Evans C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine. 26:1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3
  27. Saleem A, Walshe-Roussel B, Harris C, Asim M, Tamayo C, Sit S and Arnason JT. (2009). Characterisation of phenolics in Flor-$Essence^{(R)}$: A compound herbal product and its contributing herbs. Phytochemical Analysis. 20:395-401. https://doi.org/10.1002/pca.1139
  28. Sato Y, Itagaki S, Kurokawa T, Ogura J, Kobayashi M, Hirano T, Sugawara M and Iseki K. (2011). In vitro and in vivo antioxidant properties of chlorogenic acid and caffeic acid. International Journal of Pharmaceutics. 403:136-138. https://doi.org/10.1016/j.ijpharm.2010.09.035
  29. Seo JS, Lee TH, Lee SM, Lee SE, Seong NS and Kim J. (2009). Inhibitory effects of methanolic extracts of medicinal plants on nitric oxide production in activated macrophage RAW 264.7 cells. Korean Journal of Medicinal Crop Science. 17:173-178.
  30. Shahidi F, Janitha PK and Wanasundara PD. (1992). Phenolic antioxidants. Critical Reviews in Food Science and Nutrition. 32:67-103. https://doi.org/10.1080/10408399209527581
  31. Tezuka Y, Yamamoto K, Awale S, Lia F, Yomoda S and Kadota S. (2013). Anti-austeric activity of phenolic constituents of seeds of Arctium lappa. Natural Product Communications. 8:463-466.
  32. Tian X, Sui S, Huang J, Bai JP, Ren TS and Zhao QC. (2014). Neuroprotective effects of Arctium lappa L. roots against glutamate-induced oxidative stress by inhibiting phosphorylation of p38, JNK and ERK 1/2 MAPKs in PC12 cells. Environmental Toxicology and Pharmacology. 38:189-198. https://doi.org/10.1016/j.etap.2014.05.017
  33. Vuong QV, Goldsmith CD, Dang TT, Nguyen VT, Bhuyan DJ, Sadeqzadeh E, Scarlett CJ and Bowyer MC. (2014). Optimisation of ultrasound-assisted extraction conditions for phenolic content and antioxidant capacity from Euphorbia tirucalli using response surface methodology. Antioxidants. 3:604-617. https://doi.org/10.3390/antiox3030604
  34. Wang P, Wang B, Chung S, Wu Y, Henning SM and Vadgama JV. (2014). Increased chemopreventive effect by combining arctigenin, green tea polyphenol and curcumin in prostate and breast cancer cells. RSC Advances. 4:35242-35250. https://doi.org/10.1039/C4RA06616B
  35. Xu JG, Hu QP and Liu Y. (2012). Antioxidant and DNAprotective activities of chlorogenic acid isomers. Journal of Agricultural and Food Chemistry. 60:11625-11630. https://doi.org/10.1021/jf303771s
  36. Yeum KJ, Aldini G, Chung HY, Krinsky NI and Russell RM. (2003). The activities of antioxidant nutrients in human plasma depend on the localization of attacking radical species. The Journal of Nutrition. 133:2688-2691. https://doi.org/10.1093/jn/133.8.2688
  37. Zhao F, Wang L and Liu K. (2009). In vitro anti-inflammatory effects of arctigenin, a lignan from Arctium lappa L., through inhibition on iNOS pathway. Journal of Ethnopharmacology. 122:457-462. https://doi.org/10.1016/j.jep.2009.01.038