Toxicological Research

  • 제32권4호
  • /
  • Pages.311-316
  • /
  • 2016
  • /
  • 1976-8257(pISSN)
  • /
  • 2234-2753(eISSN)
한국독성학회 (Korean Society of Toxicology & Korea Environmental Mutagen Society)
권호 표지
DOI QR코드

DOI QR Code

Skin Corrosion and Irritation Test of Nanoparticles Using Reconstructed Three-Dimensional Human Skin Model, EpiDermTM

  • Kim, Hyejin (College of Pharmacy, Dongduk Women's University) ;
  • Choi, Jonghye (College of Pharmacy, Dongduk Women's University) ;
  • Lee, Handule (College of Pharmacy, Dongduk Women's University) ;
  • Park, Juyoung (College of Pharmacy, Dongduk Women's University) ;
  • Yoon, Byung-Il (College of Veterinary Medicine, Kangwon National University) ;
  • Jin, Seon Mi (College of Medicine, Eulji University) ;
  • Park, Kwangsik (College of Pharmacy, Dongduk Women's University)
  • 투고 : 2016.05.24
  • 심사 : 2016.08.18
  • 발행 : 2016.10.15
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Effects of nanoparticles (NPs) on skin corrosion and irritation using three-dimensional human skin models were investigated based on the test guidelines of Organization for Economic Co-operation and Development (OECD TG431 and TG439). EpiDerm$^{TM}$ skin was incubated with NPs including those harboring iron (FeNPs), aluminum oxide (AlNPs), titanium oxide (TNPs), and silver (AgNPs) for a defined time according to the test guidelines. Cell viabilities of EpiDerm$^{TM}$ skins were measured by the 3-(4, 5-dimethylthiazol-2-yl)-2.5-diphenyltetrazolium bromide based method. FeNPs, AlNPs, TNPs, and AgNPs were non-corrosive because the viability was more than 50% after 3 min exposure and more than 15% after 60 min exposure, which are the non-corrosive criteria. All NPs were also non-irritants, based on viability exceeding 50% after 60 min exposure and 42 hr post-incubation. Release of interleukin 1-alpha and histopathological analysis supported the cell viability results. These findings suggest that FeNPs, AlNPs, TNPs, and AgNPs are 'non-corrosive' and 'non-irritant' to human skin by a globally harmonized classification system.

키워드

참고문헌

  1. OECD (2002) Test No. 404: Acute dermal irritation/corrosion, OECD guidelines for the testing of chemicals, section 4. Available from: http://dx.doi.org/10.1787/9789264070622-en.
  2. OECD (2015) Test No. 404: Acute dermal irritation/corrosion, OECD guidelines for the testing of chemicals, section 4. Available from: http://dx.doi.org/10.1787/9789264242678-en.
  3. Murthy, P.B., Kishore, A.S. and Surekha, P. (2012) Assessment of in vitro skin irritation potential of nanoparticles: RHE model. Methods Mol. Biol., 926, 219-234. https://doi.org/10.1007/978-1-62703-002-1_16
  4. Basketter, D., Jirova, D. and Kandarova, H. (2012) Review of skin irritation/corrosion hazards on the basis of human data: A regulatory perspective. Interdiscip. Toxicol., 5, 98-104.
  5. OECD (2015) Test No. 431: In vitro skin corrosion: reconstructed human epidermis (RHE) test method, OECD guidelines for the testing of chemicals, section 4. Available from: http://dx.doi.org/10.1787/9789264242753-en.
  6. OECD (2015) Test No. 439: In vitro skin irritation: reconstructed human epidermis test method, OECD guidelines for the testing of chemicals, section 4. Available from: http:// dx.doi.org/10.1787/9789264242845-en.
  7. Hofmann-Amtenbrink, M., Grainger, D.W. and Hofmann, H. (2015) Nanoparticles in medicine: current challenges facing inorganic nanoparticle toxicity assessments and standardizations. Nanomedicine, 11, 1689-1694. https://doi.org/10.1016/j.nano.2015.05.005
  8. Hussain, I., Singh, N.B., Singh, A., Singh, H. and Singh, S.C. (2016) Green synthesis of nanoparticles and its potential application. Biotechnol. Lett., 38, 545-560. https://doi.org/10.1007/s10529-015-2026-7
  9. Franken, A., Eloff, F.C., Du Plessis, J. and Du Plessis, J.L. (2015) In vitro permeation of metals through human skin: a review and recommendations. Chem. Res. Toxicol., 28, 2237-2249. https://doi.org/10.1021/acs.chemrestox.5b00421
  10. Kim, J.S., Song, K.S., Sung, J.H., Ryu, H.R., Choi, B.G., Cho, H.S., Lee, J.K. and Yu, I.J. (2013) Genotoxicity, acute oral and dermal toxicity, eye and dermalirritation and corrosion and skin sensitisation evaluation of silvernanoparticles. Nanotoxicology, 7, 953-960. https://doi.org/10.3109/17435390.2012.676099
  11. Paladini, F., Sannino, A. and Pollini, M. (2014) In vivo testing of silver treated fibers for the evaluation of skin irritation effect and hypoallergenicity. J. Biomed. Mater. Res. Part B Appl. Biomater., 102, 1031-1037. https://doi.org/10.1002/jbm.b.33085
  12. Samberg, M.E., Oldenburg, S.J. and Monteiro-Riviere, N.A. (2010) Evaluation of silver nanoparticle toxicity in skin in vivo and keratinocytes in vitro. Environ. Health Perspect., 118, 407-413.
  13. Park, Y.H., Jeong, S.H., Yi, S.M., Choi, B.H., Kim, Y.R., Kim, I.K., Kim, M.K. and Son, S.W. (2011) Analysis for the potential of polystyrene and TiO2 nanoparticles to induce skin irritation, phototoxicity, and sensitization. Toxicol. In Vitro, 25, 1863-1869. https://doi.org/10.1016/j.tiv.2011.05.022
  14. Jeong, S.H., Park, Y.H., Choi, B.H., Kim, J.H., Sohn, K.H., Park, K.L., Kim, M.K. and Son, S.W. (2010) Assessment of the skin irritation potential of quantum dot nanoparticles using a human skinequivalent model. J. Dermatol. Sci., 59, 147-148. https://doi.org/10.1016/j.jdermsci.2010.06.002
  15. Park, Y.H., Kim, J.N., Jeong, S.H., Choi, J.E., Lee, S.H., Choi, B.H., Lee, J.P., Sohn, K.H., Park, K.L., Kim, M.K. and Son, S.W. (2010) Assessment of dermal toxicity of nanosilica using cultured keratinocytes, a human skin equivalent model and an in vivo model. Toxicology, 267, 178-181. https://doi.org/10.1016/j.tox.2009.10.011
  16. Contado, C. (2015) Nanomaterials in consumer products: a challenging analytical problem. Front. Chem., 3, 48.
  17. Stark, W.J., Stoessel, P.R., Wohlleben, W. and Hafner, A. (2015) Industrial applications of nanoparticles. Chem. Soc. Rev., 44, 5793-5805. https://doi.org/10.1039/C4CS00362D
  18. Khalili Fard, J., Jafari, S. and Eghbal, M.A. (2015) A review of molecular mechanisms involved in toxicity of nanoparticles. Adv. Pharm. Bull., 5, 447-454. https://doi.org/10.15171/apb.2015.061
  19. Chen, G., Vijver, M.G. and Peijnenburg, W.J. (2015) Summary and analysis of the currently existing literature data on metal-based nanoparticles published for selected aquatic organisms: applicability for toxicity prediction by (Q)SARs. Altern. Lab. Anim., 43, 221-240.
  20. Yin, J.J., Liu, J., Ehrenshaft, M., Roberts, J.E., Fu, P.P., Mason, R.P. and Zhao, B. (2012) Phototoxicity of nano titanium dioxides in HaCaT keratinocytes--generation of reactive oxygen species and cell damage. Toxicol. Appl. Pharmacol., 263, 81-88. https://doi.org/10.1016/j.taap.2012.06.001
  21. Monteiro-Riviere, N.A., Wiench, K., Landsiedel, R., Schulte, S., Inman, A.O. and Riviere, J.E. (2011) Safety evaluation of sunscreen formulations containing titanium dioxide and zinc oxide nanoparticles in UVB sunburned skin: an in vitro and in vivo study. Toxicol. Sci., 123, 264-280. https://doi.org/10.1093/toxsci/kfr148
  22. Ahamed, M., Alhadlaq, H.A., Alam, J., Khan, M.A., Ali, D. and Alarafi, S. (2013) Iron oxide nanoparticle-induced oxidative stress and genotoxicity in human skin epithelial and lung epithelial cell lines. Curr. Pharm. Des., 19, 6681-6690. https://doi.org/10.2174/1381612811319370011
  23. Astanina, K., Simon, Y., Cavelius, C., Petry, S., Kraegeloh, A. and Kiemer A.K. (2014) Superparamagnetic iron oxide nanoparticles impair endothelial integrity and inhibit nitric oxide production. Acta Biomater., 10, 4896-4911. https://doi.org/10.1016/j.actbio.2014.07.027
  24. Mesarosova, M., Kozics, K., Babelova, A., Regendova, E., Pastorek, M., Vnukova, D., Buliakova, B., Razga, F. and Gabelova, A. (2014) The role of reactive oxygen species in the genotoxicity of surface-modified magnetite nanoparticles. Toxicol. Lett., 226, 303-313. https://doi.org/10.1016/j.toxlet.2014.02.025