Ocean and Polar Research

Korea Institute of Ocean Science & Technology (한국해양과학기술원)
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Antioxidant Capacity of Crude Extract and Its Solvent Fractions of Arctic Terrestrial Plant Ranunculus heperporeus

북극식물 Ranunculus hyperboreus의 추출물과 용매분획물의 항산화 활성

  • Lee, Jung Im (Division of Marine Bioscience, Korea Maritime and Ocean University) ;
  • Kim, Hojun (Division of Marine Bioscience, Korea Maritime and Ocean University) ;
  • Seo, Hyo-Won (National Institute of Highland Agriculture, RDA) ;
  • Kong, Chang-Suk (Department of Food and Nutrition, College of Medical and Life Sciences Silla University) ;
  • Seo, Youngwan (Division of Marine Bioscience, Korea Maritime and Ocean University)
  • 이정임 (한국해양대학교 해양생명과학부) ;
  • 김호준 (한국해양대학교 해양생명과학부) ;
  • 서효원 (농촌진흥청 고령지농업연구소) ;
  • 공창숙 (신라대학교 의생명과학대학 식품영양학과) ;
  • 서영완 (한국해양대학교 해양생명과학부)
  • Received : 2016.07.10
  • Accepted : 2016.09.09
  • Published : 2016.09.30
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Abstract

In this study, antioxidative potentials of the crude extract and its four solvent fractions from the Arctic terrestrial plant Ranunculus heperporeus were evaluated by using four different activity tests, including the inhibition of intracellular reactive oxygen species (ROS) and lipid peroxidation in Raw 264.7 cells as well as determining the extent of both the scavenging of peroxynitrite ($ONOO^-$) and the oxidative damage of genomic DNA purified from Raw 264.7 cells. Based on a comparative analysis, n-BuOH, and 85% aq.MeOH solvent fractions showed good scavenging effects on the production of intracellcular ROS and inhibited membrane lipid peroxidation and DNA oxidation. In addition, n-BuOH and 85% aq.MeOH fractions exhibited good scavenging effects on both authentic peroxynitrite and one generated from SIN-1. Among the samples tested, the n-BuOH fraction revealed the strongest antioxidant effect.

본 연구에서는 북극 육상식물인 R. hyperboreus를 유기용매로 추출하여 얻어진 추출물에 대하여 세포내 ROS와 peroxynitrite 소거활성을 측정하였다. 추출물의 유의적인 소거활성을 확인한 후에 구성성분의 극성에 따른 소거효과의 변화를 보기 위하여 용매분획을 실시하여 n-hexane, 85% aq.MeOH, n-BuOH, water 분획물을 얻었다. 각 용매분획의 폴리페놀 함량을 측정하였으며 그 결과 n-hexane, water, 85% aq.MeOH, n-BuOH 분획의 순서대로 폴리페놀 함량이 증가하였다. 각 용매분획에 대한 항산화 활성을 검색한 결과 n-hexane 분획의 경우에는 세포생존율에 기인한 어느 정도의 활성변화는 있었으나 대체적으로 소거 활성은 폴리페놀 함량에 비례하였다. 폴리페놀 함량이 가장 높은 n-BuOH 분획물이 가장 좋은 항산화활성을 나타내었으며 그 다음 번째로 폴리페놀 함량이 높은 85% aq.MeOH 분획물이 두 번째로 좋은 항산화활성을 나타내었다.

Keywords

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